Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum

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S-type lectins from Bufo arenarum ovary and blastula embryos (8-16 blastomeres) were purified. These proteins showed p-galactoside-binding specificity, Caw-independency and the requirement of thiol-reducing agents to maintain their hemagglutinating activity. Lectin activity showed to be developmenta...

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Detalles Bibliográficos
Autor principal: Elola, María Teresa
Formato: Tesis Doctoral
Lenguaje:Español
Publicado: 1995
Acceso en línea:https://hdl.handle.net/20.500.12110/tesis_n2816_Elola
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:tesis_n2816_Elola2023-10-03T12:33:27Z Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum Detection, quantification and characterization of ß-Galactoside-binding lectins from Bufo arenarum Elola, María Teresa S-type lectins from Bufo arenarum ovary and blastula embryos (8-16 blastomeres) were purified. These proteins showed p-galactoside-binding specificity, Caw-independency and the requirement of thiol-reducing agents to maintain their hemagglutinating activity. Lectin activity showed to be developmentally regulated in Bufo arenarum embryos. Subunit molecular weights proved to be of 15 kDa for both lectins. Native molecular masses were of 30 and 32 kDa, for the ovary and the blastula lectin, respectively. Both lectins showed to be homodimers with non-covalently bound subunits. Isoelectrical points were 4,O and 4,7, for the ovary and the blastula lectin, respectively. Both lectins showed very strong immunological cross-reactivity, which suggests primary sequence homologies. Immunohistochemical studies demonstrated the localization of the embryonic lectin in the fertilization envelope, yolk platelets and celullar surfaces. Biological activities such as the block of the polispenny or adhesive-antiadhesive effects were suggested for these S-type lectins. Peptide or cDNA-sequencing information would be needed to classified them as galectins, and to determine the degree of homology between both. Fil: Elola, María Teresa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 Tesis Doctoral PDF Español info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar https://hdl.handle.net/20.500.12110/tesis_n2816_Elola
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Español
orig_language_str_mv Español
description S-type lectins from Bufo arenarum ovary and blastula embryos (8-16 blastomeres) were purified. These proteins showed p-galactoside-binding specificity, Caw-independency and the requirement of thiol-reducing agents to maintain their hemagglutinating activity. Lectin activity showed to be developmentally regulated in Bufo arenarum embryos. Subunit molecular weights proved to be of 15 kDa for both lectins. Native molecular masses were of 30 and 32 kDa, for the ovary and the blastula lectin, respectively. Both lectins showed to be homodimers with non-covalently bound subunits. Isoelectrical points were 4,O and 4,7, for the ovary and the blastula lectin, respectively. Both lectins showed very strong immunological cross-reactivity, which suggests primary sequence homologies. Immunohistochemical studies demonstrated the localization of the embryonic lectin in the fertilization envelope, yolk platelets and celullar surfaces. Biological activities such as the block of the polispenny or adhesive-antiadhesive effects were suggested for these S-type lectins. Peptide or cDNA-sequencing information would be needed to classified them as galectins, and to determine the degree of homology between both.
format Tesis Doctoral
author Elola, María Teresa
spellingShingle Elola, María Teresa
Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum
author_facet Elola, María Teresa
author_sort Elola, María Teresa
title Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum
title_short Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum
title_full Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum
title_fullStr Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum
title_full_unstemmed Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum
title_sort detección, cuantificación y caracterización de lectinas ß-galactosídicas de bufo arenarum
publishDate 1995
url https://hdl.handle.net/20.500.12110/tesis_n2816_Elola
work_keys_str_mv AT elolamariateresa deteccioncuantificacionycaracterizaciondelectinasßgalactosidicasdebufoarenarum
AT elolamariateresa detectionquantificationandcharacterizationofßgalactosidebindinglectinsfrombufoarenarum
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