High affinity binding of melatonin in bovine medial basal hypothalamus

Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding s...

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Autores principales: Cardinali, D.P., Vacas, M.I., Estevez Boyer, E.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_NIS18622_v6_n9_p357_Cardinali
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