High affinity binding of melatonin in bovine medial basal hypothalamus

Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding s...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Cardinali, D.P., Vacas, M.I., Estevez Boyer, E.
Formato: JOUR
Materias:
melatonin
animal experiment
cattle
central nervous system
endocrine system
hypothalamus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_NIS18622_v6_n9_p357_Cardinali
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_NIS18622_v6_n9_p357_Cardinali
record_format dspace
spelling todo:paper_NIS18622_v6_n9_p357_Cardinali2023-10-03T16:46:06Z High affinity binding of melatonin in bovine medial basal hypothalamus Cardinali, D.P. Vacas, M.I. Estevez Boyer, E. melatonin animal experiment cattle central nervous system endocrine system hypothalamus Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding sites with a Kd=(1.2±0.4)x10-8 M in 3 successive experiments. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_NIS18622_v6_n9_p357_Cardinali
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic melatonin
animal experiment
cattle
central nervous system
endocrine system
hypothalamus
spellingShingle melatonin
animal experiment
cattle
central nervous system
endocrine system
hypothalamus
Cardinali, D.P.
Vacas, M.I.
Estevez Boyer, E.
High affinity binding of melatonin in bovine medial basal hypothalamus
topic_facet melatonin
animal experiment
cattle
central nervous system
endocrine system
hypothalamus
description Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding sites with a Kd=(1.2±0.4)x10-8 M in 3 successive experiments.
format JOUR
author Cardinali, D.P.
Vacas, M.I.
Estevez Boyer, E.
author_facet Cardinali, D.P.
Vacas, M.I.
Estevez Boyer, E.
author_sort Cardinali, D.P.
title High affinity binding of melatonin in bovine medial basal hypothalamus
title_short High affinity binding of melatonin in bovine medial basal hypothalamus
title_full High affinity binding of melatonin in bovine medial basal hypothalamus
title_fullStr High affinity binding of melatonin in bovine medial basal hypothalamus
title_full_unstemmed High affinity binding of melatonin in bovine medial basal hypothalamus
title_sort high affinity binding of melatonin in bovine medial basal hypothalamus
url http://hdl.handle.net/20.500.12110/paper_NIS18622_v6_n9_p357_Cardinali
work_keys_str_mv AT cardinalidp highaffinitybindingofmelatonininbovinemedialbasalhypothalamus
AT vacasmi highaffinitybindingofmelatonininbovinemedialbasalhypothalamus
AT estevezboyere highaffinitybindingofmelatonininbovinemedialbasalhypothalamus
_version_ 1807323611596324864

Ejemplares similares