Lipid electropore stabilization

The stabilization of pores can be studied by different approaches such as simulations in silico or experimental procedures in vivo or in vitro. The energy to open a pore in a lipid membrane can be delivered by different external stimuli. To disrupt the membrane and initiate the pore opening, this en...

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Detalles Bibliográficos
Autores principales:	Fernández, M.L., Risk, M.R.
Formato:	CHAP
Materias:	Electropermeabilization Electroporation Molecular dynamics Pore stabilization
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_97833193_v1_n_p77_Fernandez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_97833193_v1_n_p77_Fernandez
record_format	dspace
spelling	todo:paper_97833193_v1_n_p77_Fernandez2023-10-03T16:44:58Z Lipid electropore stabilization Fernández, M.L. Risk, M.R. Electropermeabilization Electroporation Molecular dynamics Pore stabilization The stabilization of pores can be studied by different approaches such as simulations in silico or experimental procedures in vivo or in vitro. The energy to open a pore in a lipid membrane can be delivered by different external stimuli. To disrupt the membrane and initiate the pore opening, this energy has to reach a threshold. Then, once the pore is open, the external stimulus can be modulated to maintain the pore stable in time. This chapter first describes the basics of electropermeabilization, a process also called electroporation, and the basics of molecular dynamics in electropermeabilization. The chapter then describes in detail the molecular changes that lead to the pore opening and evolution by molecular dynamics. The chapter focuses on molecular dynamics because this technique allows the study of pore stabilization at molecular level, the interpretation of the lipid and water molecule rearrangements that are behind this phenomenon, and the visualization of the pore at the scale of size and time, in the order of nanometers and nanoseconds, respectively. Finally, the chapter also describes other approaches where pores remain open or the permeabilized state remains stable for a period of time, such as continuum modeling, experiments in planar membranes, and experiments in cells. The objective of this selection is to relate the results obtained by molecular dynamics with those obtained experimentally, or by other types of modeling, aiming to connect the mechanisms of pore stabilization by molecular dynamics at different scales. © Springer International Publishing AG 2017. All rights are reserved. CHAP info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_97833193_v1_n_p77_Fernandez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Electropermeabilization Electroporation Molecular dynamics Pore stabilization
spellingShingle	Electropermeabilization Electroporation Molecular dynamics Pore stabilization Fernández, M.L. Risk, M.R. Lipid electropore stabilization
topic_facet	Electropermeabilization Electroporation Molecular dynamics Pore stabilization
description	The stabilization of pores can be studied by different approaches such as simulations in silico or experimental procedures in vivo or in vitro. The energy to open a pore in a lipid membrane can be delivered by different external stimuli. To disrupt the membrane and initiate the pore opening, this energy has to reach a threshold. Then, once the pore is open, the external stimulus can be modulated to maintain the pore stable in time. This chapter first describes the basics of electropermeabilization, a process also called electroporation, and the basics of molecular dynamics in electropermeabilization. The chapter then describes in detail the molecular changes that lead to the pore opening and evolution by molecular dynamics. The chapter focuses on molecular dynamics because this technique allows the study of pore stabilization at molecular level, the interpretation of the lipid and water molecule rearrangements that are behind this phenomenon, and the visualization of the pore at the scale of size and time, in the order of nanometers and nanoseconds, respectively. Finally, the chapter also describes other approaches where pores remain open or the permeabilized state remains stable for a period of time, such as continuum modeling, experiments in planar membranes, and experiments in cells. The objective of this selection is to relate the results obtained by molecular dynamics with those obtained experimentally, or by other types of modeling, aiming to connect the mechanisms of pore stabilization by molecular dynamics at different scales. © Springer International Publishing AG 2017. All rights are reserved.
format	CHAP
author	Fernández, M.L. Risk, M.R.
author_facet	Fernández, M.L. Risk, M.R.
author_sort	Fernández, M.L.
title	Lipid electropore stabilization
title_short	Lipid electropore stabilization
title_full	Lipid electropore stabilization
title_fullStr	Lipid electropore stabilization
title_full_unstemmed	Lipid electropore stabilization
title_sort	lipid electropore stabilization
url	http://hdl.handle.net/20.500.12110/paper_97833193_v1_n_p77_Fernandez
work_keys_str_mv	AT fernandezml lipidelectroporestabilization AT riskmr lipidelectroporestabilization
_version_	1782028589673218048

Lipid electropore stabilization

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