Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

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Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tube...

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Autores principales: Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M.
Formato: CHAP
Materias:
Arabidopsis pollen
Cell expansion
Pollen tube growth
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p41_Borassi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_97814939_v_n_p41_Borassi
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spelling todo:paper_97814939_v_n_p41_Borassi2023-10-03T16:43:44Z Optimized method for growing in vitro Arabidopsis thaliana pollen tubes Borassi, C. Di Giorgio, J.P. Scarpin, M.R. Muschietti, J. Estevez, J.M. Arabidopsis pollen Cell expansion Pollen tube growth Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed. © Springer Science+Business Media New York 2015. All rights are reserved. Fil:Muschietti, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. CHAP info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p41_Borassi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Arabidopsis pollen
Cell expansion
Pollen tube growth
spellingShingle Arabidopsis pollen
Cell expansion
Pollen tube growth
Borassi, C.
Di Giorgio, J.P.
Scarpin, M.R.
Muschietti, J.
Estevez, J.M.
Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
topic_facet Arabidopsis pollen
Cell expansion
Pollen tube growth
description Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed. © Springer Science+Business Media New York 2015. All rights are reserved.
format CHAP
author Borassi, C.
Di Giorgio, J.P.
Scarpin, M.R.
Muschietti, J.
Estevez, J.M.
author_facet Borassi, C.
Di Giorgio, J.P.
Scarpin, M.R.
Muschietti, J.
Estevez, J.M.
author_sort Borassi, C.
title Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
title_short Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
title_full Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
title_fullStr Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
title_full_unstemmed Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
title_sort optimized method for growing in vitro arabidopsis thaliana pollen tubes
url http://hdl.handle.net/20.500.12110/paper_97814939_v_n_p41_Borassi
work_keys_str_mv AT borassic optimizedmethodforgrowinginvitroarabidopsisthalianapollentubes
AT digiorgiojp optimizedmethodforgrowinginvitroarabidopsisthalianapollentubes
AT scarpinmr optimizedmethodforgrowinginvitroarabidopsisthalianapollentubes
AT muschiettij optimizedmethodforgrowinginvitroarabidopsisthalianapollentubes
AT estevezjm optimizedmethodforgrowinginvitroarabidopsisthalianapollentubes
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