Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nong...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_87567938_v33_n1_p171_Baieli |
| Aporte de: |
| id |
todo:paper_87567938_v33_n1_p171_Baieli |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_87567938_v33_n1_p171_Baieli2023-10-03T16:42:35Z Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey Baieli, M.F. Urtasun, N. Martinez, M.J. Hirsch, D.B. Pilosof, A.M.R. Miranda, M.V. Cascone, O. Wolman, F.J. bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide chitosan n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Adsorption Amino Acids Animals Caseins Cattle Chitosan Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nonglycosylated isoforms (aCMP A and aCMP B) and its different glycosylated forms (gCMP A and gCMP B). The most predominant carbohydrate of gCMP is N-acetylneuraminic acid (sialic acid). Here, we developed a CMP purification process based on the affinity of sialic acid for wheat germ agglutinin (WGA). After formation of chitosan beads and adsorption of WGA, the agglutinin was covalently attached with glutaraldehyde. Two matrices with different WGA density were assayed for CMP adsorption. Maximum adsorption capacities were calculated according to the Langmuir model from adsorption isotherms developed at pH 7.0, being 137.0 mg/g for the matrix with the best performance. In CMP reduction from whey, maximum removal percentage was 79% (specifically 33.7% of gCMP A and B, 75.8% of aCMP A, and 93.9% of aCMP B). The CMP was recovered as an aggregate with an overall yield of 64%. Therefore, the matrices developed are promising for CMP purification from cheese whey. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:171–180, 2017. © 2016 American Institute of Chemical Engineers Fil:Martinez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_87567938_v33_n1_p171_Baieli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide chitosan n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Adsorption Amino Acids Animals Caseins Cattle Chitosan Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins |
| spellingShingle |
bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide chitosan n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Adsorption Amino Acids Animals Caseins Cattle Chitosan Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins Baieli, M.F. Urtasun, N. Martinez, M.J. Hirsch, D.B. Pilosof, A.M.R. Miranda, M.V. Cascone, O. Wolman, F.J. Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey |
| topic_facet |
bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide chitosan n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Adsorption Amino Acids Animals Caseins Cattle Chitosan Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins |
| description |
Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nonglycosylated isoforms (aCMP A and aCMP B) and its different glycosylated forms (gCMP A and gCMP B). The most predominant carbohydrate of gCMP is N-acetylneuraminic acid (sialic acid). Here, we developed a CMP purification process based on the affinity of sialic acid for wheat germ agglutinin (WGA). After formation of chitosan beads and adsorption of WGA, the agglutinin was covalently attached with glutaraldehyde. Two matrices with different WGA density were assayed for CMP adsorption. Maximum adsorption capacities were calculated according to the Langmuir model from adsorption isotherms developed at pH 7.0, being 137.0 mg/g for the matrix with the best performance. In CMP reduction from whey, maximum removal percentage was 79% (specifically 33.7% of gCMP A and B, 75.8% of aCMP A, and 93.9% of aCMP B). The CMP was recovered as an aggregate with an overall yield of 64%. Therefore, the matrices developed are promising for CMP purification from cheese whey. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:171–180, 2017. © 2016 American Institute of Chemical Engineers |
| format |
JOUR |
| author |
Baieli, M.F. Urtasun, N. Martinez, M.J. Hirsch, D.B. Pilosof, A.M.R. Miranda, M.V. Cascone, O. Wolman, F.J. |
| author_facet |
Baieli, M.F. Urtasun, N. Martinez, M.J. Hirsch, D.B. Pilosof, A.M.R. Miranda, M.V. Cascone, O. Wolman, F.J. |
| author_sort |
Baieli, M.F. |
| title |
Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey |
| title_short |
Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey |
| title_full |
Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey |
| title_fullStr |
Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey |
| title_full_unstemmed |
Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey |
| title_sort |
affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey |
| url |
http://hdl.handle.net/20.500.12110/paper_87567938_v33_n1_p171_Baieli |
| work_keys_str_mv |
AT baielimf affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey AT urtasunn affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey AT martinezmj affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey AT hirschdb affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey AT pilosofamr affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey AT mirandamv affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey AT casconeo affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey AT wolmanfj affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey |
| _version_ |
1782024461671727104 |