Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique

Mostrar todas las versiones(2)

A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repre...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cortón, E., Piuri, M., Battaglini, F., Ruzal, S.M.
Formato:	JOUR
Materias:	Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism Reduction Fermentation Biotechnology Carbohydrate Metabolism Cells, Immobilized Hydrogen-Ion Concentration Lactobacillus casei Species Specificity Bacteria (microorganisms)
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_87567938_v16_n1_p59_Corton
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_87567938_v16_n1_p59_Corton
record_format	dspace
spelling	todo:paper_87567938_v16_n1_p59_Corton2023-10-03T16:42:33Z Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique Cortón, E. Piuri, M. Battaglini, F. Ruzal, S.M. Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism pH Reduction Fermentation Biomass Bioreactors Biotechnology Carbohydrate Metabolism Cells, Immobilized Fermentation Hydrogen-Ion Concentration Lactobacillus Lactobacillus casei Species Specificity Bacteria (microorganisms) Lactobacillus A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants. A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piuri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_87567938_v16_n1_p59_Corton
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism pH Reduction Fermentation Biomass Bioreactors Biotechnology Carbohydrate Metabolism Cells, Immobilized Fermentation Hydrogen-Ion Concentration Lactobacillus Lactobacillus casei Species Specificity Bacteria (microorganisms) Lactobacillus
spellingShingle	Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism pH Reduction Fermentation Biomass Bioreactors Biotechnology Carbohydrate Metabolism Cells, Immobilized Fermentation Hydrogen-Ion Concentration Lactobacillus Lactobacillus casei Species Specificity Bacteria (microorganisms) Lactobacillus Cortón, E. Piuri, M. Battaglini, F. Ruzal, S.M. Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique
topic_facet	Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism pH Reduction Fermentation Biomass Bioreactors Biotechnology Carbohydrate Metabolism Cells, Immobilized Fermentation Hydrogen-Ion Concentration Lactobacillus Lactobacillus casei Species Specificity Bacteria (microorganisms) Lactobacillus
description	A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants. A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants.
format	JOUR
author	Cortón, E. Piuri, M. Battaglini, F. Ruzal, S.M.
author_facet	Cortón, E. Piuri, M. Battaglini, F. Ruzal, S.M.
author_sort	Cortón, E.
title	Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique
title_short	Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique
title_full	Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique
title_fullStr	Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique
title_full_unstemmed	Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique
title_sort	characterization of lactobacillus carbohydrate fermentation activity using immobilized cell technique
url	http://hdl.handle.net/20.500.12110/paper_87567938_v16_n1_p59_Corton
work_keys_str_mv	AT cortone characterizationoflactobacilluscarbohydratefermentationactivityusingimmobilizedcelltechnique AT piurim characterizationoflactobacilluscarbohydratefermentationactivityusingimmobilizedcelltechnique AT battaglinif characterizationoflactobacilluscarbohydratefermentationactivityusingimmobilizedcelltechnique AT ruzalsm characterizationoflactobacilluscarbohydratefermentationactivityusingimmobilizedcelltechnique
_version_	1807320483425681408

Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique

Ejemplares similares