Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus
The pedunculopontine nucleus (PPN) is a component of the reticular activating system (RAS), and is involved in the activated states of waking and rapid eye movement (REM) sleep. Gamma oscillations (approximately 30-80 Hz) are evident in all PPN neurons and are mediated by high-threshold voltage-depe...
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todo:paper_87507587_v115_n9_p1402_Hyde2023-10-03T16:42:29Z Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus Hyde, J. Kezunovic, N. Urbano, F.J. Garcia-Rill, E. Arousal Calcium imaging P/Q channels calcium channel calcium channel blocking agent animal arousal article calcium imaging calcium signaling drug effect metabolism P/Q channels pedunculopontine tegmental nucleus physiology rat Sprague Dawley rat arousal calcium imaging P/Q channels Animals Calcium Channel Blockers Calcium Channels Calcium Signaling Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley The pedunculopontine nucleus (PPN) is a component of the reticular activating system (RAS), and is involved in the activated states of waking and rapid eye movement (REM) sleep. Gamma oscillations (approximately 30-80 Hz) are evident in all PPN neurons and are mediated by high-threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in dendritic compartments in synchrony with patch-clamp recorded oscillations during depolarizing current ramps. Patch-clamped 8- to 16-day-old rat PPN neurons (n = 67 out of 121) were filled with Fura 2, Bis Fura, or OGB1/CHR. This study also characterized a novel ratiometric technique using Oregon Green BAPTA-1 (OGB1) with coinjections of a new long-stokes-shift dye, Chromeo 494 (CHR). Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker cadmium, or by the combination of ω-agatoxin-IVA, a specific P/Q-type calcium channel blocker, and ω-conotoxin-GVIA, a specific N-type calcium channel blocker. The calcium transients were evident in different dendrites (suggesting channels are present throughout the dendritic tree) along the sampled length without interruption (suggesting channels are evenly distributed), and appeared to represent a summation of oscillations present in the soma. We confirm that PPN calcium channel-mediated oscillations are due to P/Q- and N-type channels, and reveal that these channels are distributed along the dendrites of PPN cells. Copyright © 2013 the American Physiological Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_87507587_v115_n9_p1402_Hyde |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arousal Calcium imaging P/Q channels calcium channel calcium channel blocking agent animal arousal article calcium imaging calcium signaling drug effect metabolism P/Q channels pedunculopontine tegmental nucleus physiology rat Sprague Dawley rat arousal calcium imaging P/Q channels Animals Calcium Channel Blockers Calcium Channels Calcium Signaling Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley |
| spellingShingle |
Arousal Calcium imaging P/Q channels calcium channel calcium channel blocking agent animal arousal article calcium imaging calcium signaling drug effect metabolism P/Q channels pedunculopontine tegmental nucleus physiology rat Sprague Dawley rat arousal calcium imaging P/Q channels Animals Calcium Channel Blockers Calcium Channels Calcium Signaling Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley Hyde, J. Kezunovic, N. Urbano, F.J. Garcia-Rill, E. Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus |
| topic_facet |
Arousal Calcium imaging P/Q channels calcium channel calcium channel blocking agent animal arousal article calcium imaging calcium signaling drug effect metabolism P/Q channels pedunculopontine tegmental nucleus physiology rat Sprague Dawley rat arousal calcium imaging P/Q channels Animals Calcium Channel Blockers Calcium Channels Calcium Signaling Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley |
| description |
The pedunculopontine nucleus (PPN) is a component of the reticular activating system (RAS), and is involved in the activated states of waking and rapid eye movement (REM) sleep. Gamma oscillations (approximately 30-80 Hz) are evident in all PPN neurons and are mediated by high-threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in dendritic compartments in synchrony with patch-clamp recorded oscillations during depolarizing current ramps. Patch-clamped 8- to 16-day-old rat PPN neurons (n = 67 out of 121) were filled with Fura 2, Bis Fura, or OGB1/CHR. This study also characterized a novel ratiometric technique using Oregon Green BAPTA-1 (OGB1) with coinjections of a new long-stokes-shift dye, Chromeo 494 (CHR). Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker cadmium, or by the combination of ω-agatoxin-IVA, a specific P/Q-type calcium channel blocker, and ω-conotoxin-GVIA, a specific N-type calcium channel blocker. The calcium transients were evident in different dendrites (suggesting channels are present throughout the dendritic tree) along the sampled length without interruption (suggesting channels are evenly distributed), and appeared to represent a summation of oscillations present in the soma. We confirm that PPN calcium channel-mediated oscillations are due to P/Q- and N-type channels, and reveal that these channels are distributed along the dendrites of PPN cells. Copyright © 2013 the American Physiological Society. |
| format |
JOUR |
| author |
Hyde, J. Kezunovic, N. Urbano, F.J. Garcia-Rill, E. |
| author_facet |
Hyde, J. Kezunovic, N. Urbano, F.J. Garcia-Rill, E. |
| author_sort |
Hyde, J. |
| title |
Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus |
| title_short |
Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus |
| title_full |
Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus |
| title_fullStr |
Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus |
| title_full_unstemmed |
Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus |
| title_sort |
spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus |
| url |
http://hdl.handle.net/20.500.12110/paper_87507587_v115_n9_p1402_Hyde |
| work_keys_str_mv |
AT hydej spatiotemporalpropertiesofhighspeedcalciumoscillationsinthepedunculopontinenucleus AT kezunovicn spatiotemporalpropertiesofhighspeedcalciumoscillationsinthepedunculopontinenucleus AT urbanofj spatiotemporalpropertiesofhighspeedcalciumoscillationsinthepedunculopontinenucleus AT garciarille spatiotemporalpropertiesofhighspeedcalciumoscillationsinthepedunculopontinenucleus |
| _version_ |
1782030397130932224 |