The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection
Dengue is the most prevalent arthropod-borne viral disease worldwide and is caused by the four dengue virus serotypes (DENV-1-4). Sequential heterologous DENV infections can be associated with severe disease manifestations. Here, we present an immunocompetent mouse model of secondary DENV infection...
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todo:paper_23523964_v20_n_p202_Talarico2023-10-03T16:41:07Z The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection Talarico, L.B. Batalle, J.P. Byrne, A.B. Brahamian, J.M. Ferretti, A. García, A.G. Mauri, A. Simonetto, C. Hijano, D.R. Lawrence, A. Acosta, P.L. Caballero, M.T. Paredes Rojas, Y. Ibañez, L.I. Melendi, G.A. Rey, F.A. Damonte, E.B. Harris, E. Polack, F.P. CD8+ T lymphocytes Dengue virus Immune response Immunocompetent mouse model Secondary infection enrofloxacin granzyme B ketamine tramadol xylazine immunoglobulin G neutralizing antibody T lymphocyte receptor virus antibody adoptive transfer Aedes albopictus alanine aminotransferase blood level alkaline phosphatase blood level animal cell animal model animal tissue antibody response antisepsis Article aspartate aminotransferase blood level bleeding time blood vessel permeability CD8+ T lymphocyte controlled study creatine kinase blood level dengue Dengue virus 1 Dengue virus 2 depilation female flow cytometry histopathology Human respiratory syncytial virus humoral immune deficiency immunoassay lactate dehydrogenase blood level male mouse nonhuman priority journal real time polymerase chain reaction RNA extraction T cell depletion thrombocyte count ultraviolet irradiation virus neutralization virus titration wound healing animal CD8+ T lymphocyte cell line classification cross reaction dengue Dengue virus disease model immunology knockout mouse lymphocyte depletion metabolism serotype severity of illness index T lymphocyte subpopulation virology virus load Animals Antibodies, Neutralizing Antibodies, Viral CD8-Positive T-Lymphocytes Cell Line Cross Reactions Dengue Dengue Virus Disease Models, Animal Immunoglobulin G Lymphocyte Depletion Mice Mice, Knockout Serogroup Severity of Illness Index T-Cell Antigen Receptor Specificity T-Lymphocyte Subsets Viral Load Dengue is the most prevalent arthropod-borne viral disease worldwide and is caused by the four dengue virus serotypes (DENV-1-4). Sequential heterologous DENV infections can be associated with severe disease manifestations. Here, we present an immunocompetent mouse model of secondary DENV infection using non mouse-adapted DENV strains to investigate the pathogenesis of severe dengue disease. C57BL/6 mice infected sequentially with DENV-1 (strain Puerto Rico/94) and DENV-2 (strain Tonga/74) developed low platelet counts, internal hemorrhages, and increase of liver enzymes. Cross-reactive CD8+ T lymphocytes were found to be necessary and sufficient for signs of severe disease by adoptively transferring of DENV-1-immune CD8+ T lymphocytes before DENV-2 challenge. Disease signs were associated with production of tumor necrosis factor (TNF)-α and elevated cytotoxicity displayed by heterotypic anti-DENV-1 CD8+ T lymphocytes. These findings highlight the critical role of heterotypic anti-DENV CD8+ T lymphocytes in manifestations of severe dengue disease. © 2017 The Authors Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ibañez, L.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_23523964_v20_n_p202_Talarico |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CD8+ T lymphocytes Dengue virus Immune response Immunocompetent mouse model Secondary infection enrofloxacin granzyme B ketamine tramadol xylazine immunoglobulin G neutralizing antibody T lymphocyte receptor virus antibody adoptive transfer Aedes albopictus alanine aminotransferase blood level alkaline phosphatase blood level animal cell animal model animal tissue antibody response antisepsis Article aspartate aminotransferase blood level bleeding time blood vessel permeability CD8+ T lymphocyte controlled study creatine kinase blood level dengue Dengue virus 1 Dengue virus 2 depilation female flow cytometry histopathology Human respiratory syncytial virus humoral immune deficiency immunoassay lactate dehydrogenase blood level male mouse nonhuman priority journal real time polymerase chain reaction RNA extraction T cell depletion thrombocyte count ultraviolet irradiation virus neutralization virus titration wound healing animal CD8+ T lymphocyte cell line classification cross reaction dengue Dengue virus disease model immunology knockout mouse lymphocyte depletion metabolism serotype severity of illness index T lymphocyte subpopulation virology virus load Animals Antibodies, Neutralizing Antibodies, Viral CD8-Positive T-Lymphocytes Cell Line Cross Reactions Dengue Dengue Virus Disease Models, Animal Immunoglobulin G Lymphocyte Depletion Mice Mice, Knockout Serogroup Severity of Illness Index T-Cell Antigen Receptor Specificity T-Lymphocyte Subsets Viral Load |
| spellingShingle |
CD8+ T lymphocytes Dengue virus Immune response Immunocompetent mouse model Secondary infection enrofloxacin granzyme B ketamine tramadol xylazine immunoglobulin G neutralizing antibody T lymphocyte receptor virus antibody adoptive transfer Aedes albopictus alanine aminotransferase blood level alkaline phosphatase blood level animal cell animal model animal tissue antibody response antisepsis Article aspartate aminotransferase blood level bleeding time blood vessel permeability CD8+ T lymphocyte controlled study creatine kinase blood level dengue Dengue virus 1 Dengue virus 2 depilation female flow cytometry histopathology Human respiratory syncytial virus humoral immune deficiency immunoassay lactate dehydrogenase blood level male mouse nonhuman priority journal real time polymerase chain reaction RNA extraction T cell depletion thrombocyte count ultraviolet irradiation virus neutralization virus titration wound healing animal CD8+ T lymphocyte cell line classification cross reaction dengue Dengue virus disease model immunology knockout mouse lymphocyte depletion metabolism serotype severity of illness index T lymphocyte subpopulation virology virus load Animals Antibodies, Neutralizing Antibodies, Viral CD8-Positive T-Lymphocytes Cell Line Cross Reactions Dengue Dengue Virus Disease Models, Animal Immunoglobulin G Lymphocyte Depletion Mice Mice, Knockout Serogroup Severity of Illness Index T-Cell Antigen Receptor Specificity T-Lymphocyte Subsets Viral Load Talarico, L.B. Batalle, J.P. Byrne, A.B. Brahamian, J.M. Ferretti, A. García, A.G. Mauri, A. Simonetto, C. Hijano, D.R. Lawrence, A. Acosta, P.L. Caballero, M.T. Paredes Rojas, Y. Ibañez, L.I. Melendi, G.A. Rey, F.A. Damonte, E.B. Harris, E. Polack, F.P. The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection |
| topic_facet |
CD8+ T lymphocytes Dengue virus Immune response Immunocompetent mouse model Secondary infection enrofloxacin granzyme B ketamine tramadol xylazine immunoglobulin G neutralizing antibody T lymphocyte receptor virus antibody adoptive transfer Aedes albopictus alanine aminotransferase blood level alkaline phosphatase blood level animal cell animal model animal tissue antibody response antisepsis Article aspartate aminotransferase blood level bleeding time blood vessel permeability CD8+ T lymphocyte controlled study creatine kinase blood level dengue Dengue virus 1 Dengue virus 2 depilation female flow cytometry histopathology Human respiratory syncytial virus humoral immune deficiency immunoassay lactate dehydrogenase blood level male mouse nonhuman priority journal real time polymerase chain reaction RNA extraction T cell depletion thrombocyte count ultraviolet irradiation virus neutralization virus titration wound healing animal CD8+ T lymphocyte cell line classification cross reaction dengue Dengue virus disease model immunology knockout mouse lymphocyte depletion metabolism serotype severity of illness index T lymphocyte subpopulation virology virus load Animals Antibodies, Neutralizing Antibodies, Viral CD8-Positive T-Lymphocytes Cell Line Cross Reactions Dengue Dengue Virus Disease Models, Animal Immunoglobulin G Lymphocyte Depletion Mice Mice, Knockout Serogroup Severity of Illness Index T-Cell Antigen Receptor Specificity T-Lymphocyte Subsets Viral Load |
| description |
Dengue is the most prevalent arthropod-borne viral disease worldwide and is caused by the four dengue virus serotypes (DENV-1-4). Sequential heterologous DENV infections can be associated with severe disease manifestations. Here, we present an immunocompetent mouse model of secondary DENV infection using non mouse-adapted DENV strains to investigate the pathogenesis of severe dengue disease. C57BL/6 mice infected sequentially with DENV-1 (strain Puerto Rico/94) and DENV-2 (strain Tonga/74) developed low platelet counts, internal hemorrhages, and increase of liver enzymes. Cross-reactive CD8+ T lymphocytes were found to be necessary and sufficient for signs of severe disease by adoptively transferring of DENV-1-immune CD8+ T lymphocytes before DENV-2 challenge. Disease signs were associated with production of tumor necrosis factor (TNF)-α and elevated cytotoxicity displayed by heterotypic anti-DENV-1 CD8+ T lymphocytes. These findings highlight the critical role of heterotypic anti-DENV CD8+ T lymphocytes in manifestations of severe dengue disease. © 2017 The Authors |
| format |
JOUR |
| author |
Talarico, L.B. Batalle, J.P. Byrne, A.B. Brahamian, J.M. Ferretti, A. García, A.G. Mauri, A. Simonetto, C. Hijano, D.R. Lawrence, A. Acosta, P.L. Caballero, M.T. Paredes Rojas, Y. Ibañez, L.I. Melendi, G.A. Rey, F.A. Damonte, E.B. Harris, E. Polack, F.P. |
| author_facet |
Talarico, L.B. Batalle, J.P. Byrne, A.B. Brahamian, J.M. Ferretti, A. García, A.G. Mauri, A. Simonetto, C. Hijano, D.R. Lawrence, A. Acosta, P.L. Caballero, M.T. Paredes Rojas, Y. Ibañez, L.I. Melendi, G.A. Rey, F.A. Damonte, E.B. Harris, E. Polack, F.P. |
| author_sort |
Talarico, L.B. |
| title |
The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection |
| title_short |
The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection |
| title_full |
The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection |
| title_fullStr |
The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection |
| title_full_unstemmed |
The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection |
| title_sort |
role of heterotypic denv-specific cd8+ t lymphocytes in an immunocompetent mouse model of secondary dengue virus infection |
| url |
http://hdl.handle.net/20.500.12110/paper_23523964_v20_n_p202_Talarico |
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