Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro
Polyfunctionalized stigmasterol derivatives, (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5,22,23-trihydroxystigmastan-6-one (compound 2), inhibit herpes simplex virus type 1 (HSV-1) replication and spreading in human epithelial cells derived from ocular tissues....
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Petrera |
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todo:paper_23146133_v2014_n_p_Petrera2023-10-03T16:40:56Z Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro Petrera, E. Níttolo, A.G. Alché, L.E. 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative gamma interferon interleukin 6 stigmasterol animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell Herpes simplex virus 1 inflammation metabolism mouse Vero cell line virus replication Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication Polyfunctionalized stigmasterol derivatives, (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5,22,23-trihydroxystigmastan-6-one (compound 2), inhibit herpes simplex virus type 1 (HSV-1) replication and spreading in human epithelial cells derived from ocular tissues. Both compounds reduce the incidence and severity of lesions in a murine model of herpetic stromal keratitis when administered in different treatment modalities. Since encephalitis caused by HSV-1 is another immunopathology of viral origin, we evaluate here the antiviral effect of both compounds on HSV-1 infected nervous cell lines as well as their anti-inflammatory action. We found that both stigmasterol derivatives presented low cytotoxicity in the three nervous cell lines assayed. Regarding the antiviral activity, in all cases both compounds prevented HSV-1 multiplication when added after infection, as well as virus propagation. Additionally, both compounds were able to hinder interleukin-6 and Interferon-gamma secretion induced by HSV-1 infection in Neuro-2a cells. We conclude that compounds 1 and 2 have exerted a dual antiviral and anti-inflammatory effect in HSV-1 infected nervous cell lines, which makes them interesting molecules to be further studied. © 2014 Erina Petrera et al. Fil:Petrera, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Níttolo, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alché, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Petrera |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative gamma interferon interleukin 6 stigmasterol animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell Herpes simplex virus 1 inflammation metabolism mouse Vero cell line virus replication Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication |
| spellingShingle |
22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative gamma interferon interleukin 6 stigmasterol animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell Herpes simplex virus 1 inflammation metabolism mouse Vero cell line virus replication Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication Petrera, E. Níttolo, A.G. Alché, L.E. Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro |
| topic_facet |
22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative gamma interferon interleukin 6 stigmasterol animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell Herpes simplex virus 1 inflammation metabolism mouse Vero cell line virus replication Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication |
| description |
Polyfunctionalized stigmasterol derivatives, (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5,22,23-trihydroxystigmastan-6-one (compound 2), inhibit herpes simplex virus type 1 (HSV-1) replication and spreading in human epithelial cells derived from ocular tissues. Both compounds reduce the incidence and severity of lesions in a murine model of herpetic stromal keratitis when administered in different treatment modalities. Since encephalitis caused by HSV-1 is another immunopathology of viral origin, we evaluate here the antiviral effect of both compounds on HSV-1 infected nervous cell lines as well as their anti-inflammatory action. We found that both stigmasterol derivatives presented low cytotoxicity in the three nervous cell lines assayed. Regarding the antiviral activity, in all cases both compounds prevented HSV-1 multiplication when added after infection, as well as virus propagation. Additionally, both compounds were able to hinder interleukin-6 and Interferon-gamma secretion induced by HSV-1 infection in Neuro-2a cells. We conclude that compounds 1 and 2 have exerted a dual antiviral and anti-inflammatory effect in HSV-1 infected nervous cell lines, which makes them interesting molecules to be further studied. © 2014 Erina Petrera et al. |
| format |
JOUR |
| author |
Petrera, E. Níttolo, A.G. Alché, L.E. |
| author_facet |
Petrera, E. Níttolo, A.G. Alché, L.E. |
| author_sort |
Petrera, E. |
| title |
Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro |
| title_short |
Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro |
| title_full |
Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro |
| title_fullStr |
Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro |
| title_full_unstemmed |
Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro |
| title_sort |
antiviral action of synthetic stigmasterol derivatives on herpes simplex virus replication in nervous cells in vitro |
| url |
http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Petrera |
| work_keys_str_mv |
AT petrerae antiviralactionofsyntheticstigmasterolderivativesonherpessimplexvirusreplicationinnervouscellsinvitro AT nittoloag antiviralactionofsyntheticstigmasterolderivativesonherpessimplexvirusreplicationinnervouscellsinvitro AT alchele antiviralactionofsyntheticstigmasterolderivativesonherpessimplexvirusreplicationinnervouscellsinvitro |
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