Co-imaging extrinsic, intrinsic and effector caspase activity by fluorescence anisotropy microscopy

In order to overcome intercellular variability and thereby effectively assess signal propagation in biological networks it is imperative to simultaneously quantify multiple biological observables in single living cells. While fluorescent biosensors have been the tool of choice to monitor the dynamic...

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Detalles Bibliográficos
Autores principales: Corbat, A.A., Schuermann, K.C., Liguzinski, P., Radon, Y., Bastiaens, P.I.H., Verveer, P.J., Grecco, H.E.
Formato: JOUR
Materias:
Anisotropy FRET biosensor
Apoptotic network
Caspase activity
Co-monitoring
Imaging
Polarization microscopy
caspase 3
caspase 8
caspase 9
effector caspase
anisotropy
anisotropy forster resonant energy transfer based biosensor
apoptosis
Article
controlled study
correlation analysis
enzyme activation
enzyme activity
fluorescence anisotropy microscopy
human
human cell
microscopy
molecular model
priority journal
simulation
fluorescence microscopy
fluorescence polarization
fluorescence resonance energy transfer
genetic procedures
HeLa cell line
metabolism
procedures
signal transduction
Apoptosis
Biosensing Techniques
Caspases, Effector
Enzyme Activation
Fluorescence Polarization
Fluorescence Resonance Energy Transfer
HeLa Cells
Humans
Microscopy, Fluorescence
Signal Transduction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_22132317_v19_n_p210_Corbat
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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