Novel red fluorophores with superior performance in STED microscopy
In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine ba...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_21922853_v1_n1_p1_Wurm |
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todo:paper_21922853_v1_n1_p1_Wurm2023-10-03T16:40:20Z Novel red fluorophores with superior performance in STED microscopy Wurm, C.A. Kolmakov, K. Göttfert, F. Ta, H. Bossi, M. Schill, H. Berning, S. Jakobs, S. Donnert, G. Belov, V.N. Hell, S.W. Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Confocal Nanoscopy Rhodamines STED Stimulated emission depletion Super resolution Fluorescence Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_21922853_v1_n1_p1_Wurm |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Confocal Nanoscopy Rhodamines STED Stimulated emission depletion Super resolution Fluorescence Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission |
| spellingShingle |
Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Confocal Nanoscopy Rhodamines STED Stimulated emission depletion Super resolution Fluorescence Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission Wurm, C.A. Kolmakov, K. Göttfert, F. Ta, H. Bossi, M. Schill, H. Berning, S. Jakobs, S. Donnert, G. Belov, V.N. Hell, S.W. Novel red fluorophores with superior performance in STED microscopy |
| topic_facet |
Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Confocal Nanoscopy Rhodamines STED Stimulated emission depletion Super resolution Fluorescence Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission |
| description |
In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al. |
| format |
JOUR |
| author |
Wurm, C.A. Kolmakov, K. Göttfert, F. Ta, H. Bossi, M. Schill, H. Berning, S. Jakobs, S. Donnert, G. Belov, V.N. Hell, S.W. |
| author_facet |
Wurm, C.A. Kolmakov, K. Göttfert, F. Ta, H. Bossi, M. Schill, H. Berning, S. Jakobs, S. Donnert, G. Belov, V.N. Hell, S.W. |
| author_sort |
Wurm, C.A. |
| title |
Novel red fluorophores with superior performance in STED microscopy |
| title_short |
Novel red fluorophores with superior performance in STED microscopy |
| title_full |
Novel red fluorophores with superior performance in STED microscopy |
| title_fullStr |
Novel red fluorophores with superior performance in STED microscopy |
| title_full_unstemmed |
Novel red fluorophores with superior performance in STED microscopy |
| title_sort |
novel red fluorophores with superior performance in sted microscopy |
| url |
http://hdl.handle.net/20.500.12110/paper_21922853_v1_n1_p1_Wurm |
| work_keys_str_mv |
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