Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology
Polyamines are essential for Trypanosoma cruzi, the causative agent of Chagas disease. As T. cruzi behaves as a natural auxotrophic organism, it relies on host polyamines biosynthesis. In this paper we obtained a double-transfected T. cruzi parasite that expresses the green fluorescent protein (GFP)...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_20900406_v2011_n1_p_Barclay |
| Aporte de: |
| id |
todo:paper_20900406_v2011_n1_p_Barclay |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_20900406_v2011_n1_p_Barclay2023-10-03T16:39:19Z Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology Barclay, J.J. Morosi, L.G. Vanrell, M.C. Trejo, E.C. Romano, P.S. Carrillo, C. green fluorescent protein ornithine ornithine decarboxylase polyamine animal cell article auxotrophy Chagas disease controlled study enzyme activity enzyme kinetics fluorescence analysis genetic transfection nonhuman parasite survival parasite transmission pathogenesis priority journal protein expression Trypanosoma cruzi Vero cell Trypanosoma cruzi Polyamines are essential for Trypanosoma cruzi, the causative agent of Chagas disease. As T. cruzi behaves as a natural auxotrophic organism, it relies on host polyamines biosynthesis. In this paper we obtained a double-transfected T. cruzi parasite that expresses the green fluorescent protein (GFP) and a heterologous ornithine decarboxylase (ODC), used itself as a novel selectable marker. These autotrophic and fluorescent parasites were characterized; the ODC presented an apparent Km for ornithine of 0.51 0.16 mM and an estimated Vvalue of 476.2 nmoles/h/mg of protein. These expressing ODC parasites showed higher metacyclogenesis capacity than the auxotrophic counterpart, supporting the idea that polyamines are engaged in this process. This double-transfected T. cruzi parasite results in a powerful tool - easy to follow by its fluorescence - to study the role of polyamines in Chagas disease pathology and in related processes such as parasite survival, invasion, proliferation, metacyclogenesis, and tissue spreading. © 2011 Jeremías José Barclay et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20900406_v2011_n1_p_Barclay |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
green fluorescent protein ornithine ornithine decarboxylase polyamine animal cell article auxotrophy Chagas disease controlled study enzyme activity enzyme kinetics fluorescence analysis genetic transfection nonhuman parasite survival parasite transmission pathogenesis priority journal protein expression Trypanosoma cruzi Vero cell Trypanosoma cruzi |
| spellingShingle |
green fluorescent protein ornithine ornithine decarboxylase polyamine animal cell article auxotrophy Chagas disease controlled study enzyme activity enzyme kinetics fluorescence analysis genetic transfection nonhuman parasite survival parasite transmission pathogenesis priority journal protein expression Trypanosoma cruzi Vero cell Trypanosoma cruzi Barclay, J.J. Morosi, L.G. Vanrell, M.C. Trejo, E.C. Romano, P.S. Carrillo, C. Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology |
| topic_facet |
green fluorescent protein ornithine ornithine decarboxylase polyamine animal cell article auxotrophy Chagas disease controlled study enzyme activity enzyme kinetics fluorescence analysis genetic transfection nonhuman parasite survival parasite transmission pathogenesis priority journal protein expression Trypanosoma cruzi Vero cell Trypanosoma cruzi |
| description |
Polyamines are essential for Trypanosoma cruzi, the causative agent of Chagas disease. As T. cruzi behaves as a natural auxotrophic organism, it relies on host polyamines biosynthesis. In this paper we obtained a double-transfected T. cruzi parasite that expresses the green fluorescent protein (GFP) and a heterologous ornithine decarboxylase (ODC), used itself as a novel selectable marker. These autotrophic and fluorescent parasites were characterized; the ODC presented an apparent Km for ornithine of 0.51 0.16 mM and an estimated Vvalue of 476.2 nmoles/h/mg of protein. These expressing ODC parasites showed higher metacyclogenesis capacity than the auxotrophic counterpart, supporting the idea that polyamines are engaged in this process. This double-transfected T. cruzi parasite results in a powerful tool - easy to follow by its fluorescence - to study the role of polyamines in Chagas disease pathology and in related processes such as parasite survival, invasion, proliferation, metacyclogenesis, and tissue spreading. © 2011 Jeremías José Barclay et al. |
| format |
JOUR |
| author |
Barclay, J.J. Morosi, L.G. Vanrell, M.C. Trejo, E.C. Romano, P.S. Carrillo, C. |
| author_facet |
Barclay, J.J. Morosi, L.G. Vanrell, M.C. Trejo, E.C. Romano, P.S. Carrillo, C. |
| author_sort |
Barclay, J.J. |
| title |
Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology |
| title_short |
Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology |
| title_full |
Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology |
| title_fullStr |
Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology |
| title_full_unstemmed |
Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology |
| title_sort |
trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology |
| url |
http://hdl.handle.net/20.500.12110/paper_20900406_v2011_n1_p_Barclay |
| work_keys_str_mv |
AT barclayjj trypanosomacruzicoexpressingornithinedecarboxylaseandgreenfluorescenceproteinsasatooltostudytheroleofpolyaminesinchagasdiseasepathology AT morosilg trypanosomacruzicoexpressingornithinedecarboxylaseandgreenfluorescenceproteinsasatooltostudytheroleofpolyaminesinchagasdiseasepathology AT vanrellmc trypanosomacruzicoexpressingornithinedecarboxylaseandgreenfluorescenceproteinsasatooltostudytheroleofpolyaminesinchagasdiseasepathology AT trejoec trypanosomacruzicoexpressingornithinedecarboxylaseandgreenfluorescenceproteinsasatooltostudytheroleofpolyaminesinchagasdiseasepathology AT romanops trypanosomacruzicoexpressingornithinedecarboxylaseandgreenfluorescenceproteinsasatooltostudytheroleofpolyaminesinchagasdiseasepathology AT carrilloc trypanosomacruzicoexpressingornithinedecarboxylaseandgreenfluorescenceproteinsasatooltostudytheroleofpolyaminesinchagasdiseasepathology |
| _version_ |
1807315362009579520 |