Intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (PPN)
The pedunculopontine nucleus is a part of the reticular activating system, and is active during waking and REM sleep. Previous results showed that all PPN cells tested fired maximally at gamma frequencies when depolarized. This intrinsic membrane property was shown to be mediated by high-threshold N...
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todo:paper_2051817X_v4_n12_p_Luster2023-10-03T16:38:53Z Intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (PPN) Luster, B.R. Urbano, F.J. Garcia-Rill, E. Arousal Ca2+ channels CaMKII cAMP-PKA gamma oscillations N-Type P/Q-type calcium calmodulin dependent protein kinase II calcium channel N type cyclic AMP cyclic AMP dependent protein kinase animal cytology gamma rhythm metabolism nerve cell pedunculopontine tegmental nucleus physiology rat second messenger Sprague Dawley rat Animals Calcium Channels, N-Type Calcium-Calmodulin-Dependent Protein Kinase Type 2 Cyclic AMP Cyclic AMP-Dependent Protein Kinases Gamma Rhythm Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley Second Messenger Systems The pedunculopontine nucleus is a part of the reticular activating system, and is active during waking and REM sleep. Previous results showed that all PPN cells tested fired maximally at gamma frequencies when depolarized. This intrinsic membrane property was shown to be mediated by high-threshold N- and P/Q-type Ca2+ channels. Recent studies show that the PPN contains three independent populations of neurons which can generate gamma band oscillations through only N-type channels, only P/Q-type channels, or both N- and P/Q-type channels. This study investigated the intracellular mechanisms modulating gamma band activity in each population of neurons. We performed in vitro patch-clamp recordings of PPN neurons from Sprague–Dawley rat pups, and applied 1-sec ramps to induce intrinsic membrane oscillations. Our results show that there are two pathways modulating gamma band activity in PPN neurons. We describe populations of neurons mediating gamma band activity through only N-type channels and the cAMP/PKA pathway (presumed “REM-on” neurons), through only P/Q-type channels and the CaMKII pathway (presumed “Wake-on” neurons), and a third population which can mediate gamma activity through both N-type channels and cAMP/PK and P/Q-type channels and CaMKII (presumed “Wake/REM-on” neurons). These novel results suggest that PPN gamma oscillations are modulated by two independent pathways related to different Ca2+ channel types. © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_2051817X_v4_n12_p_Luster |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arousal Ca2+ channels CaMKII cAMP-PKA gamma oscillations N-Type P/Q-type calcium calmodulin dependent protein kinase II calcium channel N type cyclic AMP cyclic AMP dependent protein kinase animal cytology gamma rhythm metabolism nerve cell pedunculopontine tegmental nucleus physiology rat second messenger Sprague Dawley rat Animals Calcium Channels, N-Type Calcium-Calmodulin-Dependent Protein Kinase Type 2 Cyclic AMP Cyclic AMP-Dependent Protein Kinases Gamma Rhythm Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley Second Messenger Systems |
| spellingShingle |
Arousal Ca2+ channels CaMKII cAMP-PKA gamma oscillations N-Type P/Q-type calcium calmodulin dependent protein kinase II calcium channel N type cyclic AMP cyclic AMP dependent protein kinase animal cytology gamma rhythm metabolism nerve cell pedunculopontine tegmental nucleus physiology rat second messenger Sprague Dawley rat Animals Calcium Channels, N-Type Calcium-Calmodulin-Dependent Protein Kinase Type 2 Cyclic AMP Cyclic AMP-Dependent Protein Kinases Gamma Rhythm Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley Second Messenger Systems Luster, B.R. Urbano, F.J. Garcia-Rill, E. Intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (PPN) |
| topic_facet |
Arousal Ca2+ channels CaMKII cAMP-PKA gamma oscillations N-Type P/Q-type calcium calmodulin dependent protein kinase II calcium channel N type cyclic AMP cyclic AMP dependent protein kinase animal cytology gamma rhythm metabolism nerve cell pedunculopontine tegmental nucleus physiology rat second messenger Sprague Dawley rat Animals Calcium Channels, N-Type Calcium-Calmodulin-Dependent Protein Kinase Type 2 Cyclic AMP Cyclic AMP-Dependent Protein Kinases Gamma Rhythm Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley Second Messenger Systems |
| description |
The pedunculopontine nucleus is a part of the reticular activating system, and is active during waking and REM sleep. Previous results showed that all PPN cells tested fired maximally at gamma frequencies when depolarized. This intrinsic membrane property was shown to be mediated by high-threshold N- and P/Q-type Ca2+ channels. Recent studies show that the PPN contains three independent populations of neurons which can generate gamma band oscillations through only N-type channels, only P/Q-type channels, or both N- and P/Q-type channels. This study investigated the intracellular mechanisms modulating gamma band activity in each population of neurons. We performed in vitro patch-clamp recordings of PPN neurons from Sprague–Dawley rat pups, and applied 1-sec ramps to induce intrinsic membrane oscillations. Our results show that there are two pathways modulating gamma band activity in PPN neurons. We describe populations of neurons mediating gamma band activity through only N-type channels and the cAMP/PKA pathway (presumed “REM-on” neurons), through only P/Q-type channels and the CaMKII pathway (presumed “Wake-on” neurons), and a third population which can mediate gamma activity through both N-type channels and cAMP/PK and P/Q-type channels and CaMKII (presumed “Wake/REM-on” neurons). These novel results suggest that PPN gamma oscillations are modulated by two independent pathways related to different Ca2+ channel types. © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. |
| format |
JOUR |
| author |
Luster, B.R. Urbano, F.J. Garcia-Rill, E. |
| author_facet |
Luster, B.R. Urbano, F.J. Garcia-Rill, E. |
| author_sort |
Luster, B.R. |
| title |
Intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (PPN) |
| title_short |
Intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (PPN) |
| title_full |
Intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (PPN) |
| title_fullStr |
Intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (PPN) |
| title_full_unstemmed |
Intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (PPN) |
| title_sort |
intracellular mechanisms modulating gamma band activity in the pedunculopontine nucleus (ppn) |
| url |
http://hdl.handle.net/20.500.12110/paper_2051817X_v4_n12_p_Luster |
| work_keys_str_mv |
AT lusterbr intracellularmechanismsmodulatinggammabandactivityinthepedunculopontinenucleusppn AT urbanofj intracellularmechanismsmodulatinggammabandactivityinthepedunculopontinenucleusppn AT garciarille intracellularmechanismsmodulatinggammabandactivityinthepedunculopontinenucleusppn |
| _version_ |
1807319119619424256 |