Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafis...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_2050084X_v7_n_p_LlerasForero |
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todo:paper_2050084X_v7_n_p_LlerasForero2023-10-03T16:38:45Z Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock Lleras Forero, L. Narayanan, R. Huitema, L.F.A. Vanbergen, M. Apschner, A. Peterson-Maduro, J. Logister, I. Valentin, G. Morelli, L.G. Oates, A.C. Schulte-Merker, S. amniote Article axial patterning coccygeal vertebra controlled study DNA binding embryo embryo segmentation gene expression in situ hybridization mesoderm muscle cell myotome nonhuman notochord ossification osteoblast promoter region sclerotome stop codon teleost vertebra body zebra fish Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. © Lleras Forero et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_2050084X_v7_n_p_LlerasForero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
amniote Article axial patterning coccygeal vertebra controlled study DNA binding embryo embryo segmentation gene expression in situ hybridization mesoderm muscle cell myotome nonhuman notochord ossification osteoblast promoter region sclerotome stop codon teleost vertebra body zebra fish |
| spellingShingle |
amniote Article axial patterning coccygeal vertebra controlled study DNA binding embryo embryo segmentation gene expression in situ hybridization mesoderm muscle cell myotome nonhuman notochord ossification osteoblast promoter region sclerotome stop codon teleost vertebra body zebra fish Lleras Forero, L. Narayanan, R. Huitema, L.F.A. Vanbergen, M. Apschner, A. Peterson-Maduro, J. Logister, I. Valentin, G. Morelli, L.G. Oates, A.C. Schulte-Merker, S. Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
| topic_facet |
amniote Article axial patterning coccygeal vertebra controlled study DNA binding embryo embryo segmentation gene expression in situ hybridization mesoderm muscle cell myotome nonhuman notochord ossification osteoblast promoter region sclerotome stop codon teleost vertebra body zebra fish |
| description |
Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. © Lleras Forero et al. |
| format |
JOUR |
| author |
Lleras Forero, L. Narayanan, R. Huitema, L.F.A. Vanbergen, M. Apschner, A. Peterson-Maduro, J. Logister, I. Valentin, G. Morelli, L.G. Oates, A.C. Schulte-Merker, S. |
| author_facet |
Lleras Forero, L. Narayanan, R. Huitema, L.F.A. Vanbergen, M. Apschner, A. Peterson-Maduro, J. Logister, I. Valentin, G. Morelli, L.G. Oates, A.C. Schulte-Merker, S. |
| author_sort |
Lleras Forero, L. |
| title |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
| title_short |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
| title_full |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
| title_fullStr |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
| title_full_unstemmed |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
| title_sort |
segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
| url |
http://hdl.handle.net/20.500.12110/paper_2050084X_v7_n_p_LlerasForero |
| work_keys_str_mv |
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| _version_ |
1782026616575098880 |