Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions

Focal adhesions anchor contractile actin fibers with the extracellular matrix, sense the generated tension and respond to it by changing their morphology and composition. Here we ask how this mechanosensing is enabled at the protein-network level, given the modular assembly and multitasking of focal...

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Autores principales: Malik-Sheriff, R.S., Imtiaz, S., Grecco, H.E., Zamir, E.
Formato: JOUR
Materias:
Rho kinase
actin filament
animal
cell culture
cytology
extracellular matrix
fibroblast
focal adhesion
image processing
mechanotransduction
metabolism
physiology
rat
Actin Cytoskeleton
Animals
Cells, Cultured
Extracellular Matrix
Fibroblasts
Focal Adhesions
Image Processing, Computer-Assisted
Mechanotransduction, Cellular
Rats
rho-Associated Kinases
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_MalikSheriff
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_20452322_v8_n1_p_MalikSheriff
record_format dspace
spelling todo:paper_20452322_v8_n1_p_MalikSheriff2023-10-03T16:38:26Z Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions Malik-Sheriff, R.S. Imtiaz, S. Grecco, H.E. Zamir, E. Rho kinase actin filament animal cell culture cytology extracellular matrix fibroblast focal adhesion image processing mechanotransduction metabolism physiology rat Actin Cytoskeleton Animals Cells, Cultured Extracellular Matrix Fibroblasts Focal Adhesions Image Processing, Computer-Assisted Mechanotransduction, Cellular Rats rho-Associated Kinases Focal adhesions anchor contractile actin fibers with the extracellular matrix, sense the generated tension and respond to it by changing their morphology and composition. Here we ask how this mechanosensing is enabled at the protein-network level, given the modular assembly and multitasking of focal adhesions. To address this, we applied a sensitive 4-color live cell imaging approach, enabling monitoring patterns of molecular changes in single focal adhesions. Co-imaging zyxin, FAK, vinculin and paxillin revealed heterogeneities in their responses to Rho-associated kinase (ROCK)-mediated perturbations of actomyosin contractility. These responses were rather weakly correlated between the proteins, reflecting diverse compositional changes in different focal adhesions. This diversity is partially attributable to the location of focal adhesions, their area, molecular content and previous contractility perturbations, suggesting that integration of multiple local cues shapes differentially focal adhesion mechano-responsiveness. Importantly, the compositional changes upon ROCK perturbations exhibited distinct paths in different focal adhesions. Moreover, the protein exhibiting the strongest response to ROCK perturbations varied among different focal adhesions. The diversity in response patterns is plausibly enabled by the modular mode of focal adhesions assembly and can provide them the needed flexibility to perform multiple tasks by combining optimally a common set of multifunctional components. © 2018 The Author(s). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_MalikSheriff
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Rho kinase
actin filament
animal
cell culture
cytology
extracellular matrix
fibroblast
focal adhesion
image processing
mechanotransduction
metabolism
physiology
rat
Actin Cytoskeleton
Animals
Cells, Cultured
Extracellular Matrix
Fibroblasts
Focal Adhesions
Image Processing, Computer-Assisted
Mechanotransduction, Cellular
Rats
rho-Associated Kinases
spellingShingle Rho kinase
actin filament
animal
cell culture
cytology
extracellular matrix
fibroblast
focal adhesion
image processing
mechanotransduction
metabolism
physiology
rat
Actin Cytoskeleton
Animals
Cells, Cultured
Extracellular Matrix
Fibroblasts
Focal Adhesions
Image Processing, Computer-Assisted
Mechanotransduction, Cellular
Rats
rho-Associated Kinases
Malik-Sheriff, R.S.
Imtiaz, S.
Grecco, H.E.
Zamir, E.
Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions
topic_facet Rho kinase
actin filament
animal
cell culture
cytology
extracellular matrix
fibroblast
focal adhesion
image processing
mechanotransduction
metabolism
physiology
rat
Actin Cytoskeleton
Animals
Cells, Cultured
Extracellular Matrix
Fibroblasts
Focal Adhesions
Image Processing, Computer-Assisted
Mechanotransduction, Cellular
Rats
rho-Associated Kinases
description Focal adhesions anchor contractile actin fibers with the extracellular matrix, sense the generated tension and respond to it by changing their morphology and composition. Here we ask how this mechanosensing is enabled at the protein-network level, given the modular assembly and multitasking of focal adhesions. To address this, we applied a sensitive 4-color live cell imaging approach, enabling monitoring patterns of molecular changes in single focal adhesions. Co-imaging zyxin, FAK, vinculin and paxillin revealed heterogeneities in their responses to Rho-associated kinase (ROCK)-mediated perturbations of actomyosin contractility. These responses were rather weakly correlated between the proteins, reflecting diverse compositional changes in different focal adhesions. This diversity is partially attributable to the location of focal adhesions, their area, molecular content and previous contractility perturbations, suggesting that integration of multiple local cues shapes differentially focal adhesion mechano-responsiveness. Importantly, the compositional changes upon ROCK perturbations exhibited distinct paths in different focal adhesions. Moreover, the protein exhibiting the strongest response to ROCK perturbations varied among different focal adhesions. The diversity in response patterns is plausibly enabled by the modular mode of focal adhesions assembly and can provide them the needed flexibility to perform multiple tasks by combining optimally a common set of multifunctional components. © 2018 The Author(s).
format JOUR
author Malik-Sheriff, R.S.
Imtiaz, S.
Grecco, H.E.
Zamir, E.
author_facet Malik-Sheriff, R.S.
Imtiaz, S.
Grecco, H.E.
Zamir, E.
author_sort Malik-Sheriff, R.S.
title Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions
title_short Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions
title_full Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions
title_fullStr Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions
title_full_unstemmed Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions
title_sort diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions
url http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_MalikSheriff
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AT imtiazs diversepatternsofmolecularchangesinthemechanoresponsivenessoffocaladhesions
AT greccohe diversepatternsofmolecularchangesinthemechanoresponsivenessoffocaladhesions
AT zamire diversepatternsofmolecularchangesinthemechanoresponsivenessoffocaladhesions
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