A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity
Fasciolosis is a trematode zoonosis of interest in public health and cattle production. We report here the immunostimulatory effect of a 66 mer mucin-like peptide from Fasciola hepatica (Fhmuc), which synergizes with lipopolysaccharide (LPS) to promote dendritic cell (DC) maturation, endowing these...
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todo:paper_20452322_v7_n_p_Noya2023-10-03T16:38:21Z A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity Noya, V. Brossard, N. Rodríguez, E. Dergan-Dylon, L.S. Carmona, C. Rabinovich, G.A. Freire, T. antibody autacoid cytokine gamma interferon glycoprotein p 15095 helminth antibody immunoglobulin enhancer binding protein immunoglobulin G lipopolysaccharide mucin 1 peptide toll like receptor 4 animal biological model C57BL mouse cell polarity cytology dendritic cell drug effect Fasciola hepatica female immunology lymphocyte activation metabolism parasite pathology peritoneal cavity signal transduction species difference spleen Th1 cell vaccination Animals Antibodies Antibodies, Helminth CD11c Antigen Cell Polarity Cytokines Dendritic Cells Fasciola hepatica Female Immunoglobulin G Inflammation Mediators Interferon-gamma Lipopolysaccharides Lymphocyte Activation Mice, Inbred C57BL Models, Biological Mucin-1 NF-kappa B Parasites Peptides Peritoneal Cavity Signal Transduction Species Specificity Spleen Th1 Cells Toll-Like Receptor 4 Vaccination Fasciolosis is a trematode zoonosis of interest in public health and cattle production. We report here the immunostimulatory effect of a 66 mer mucin-like peptide from Fasciola hepatica (Fhmuc), which synergizes with lipopolysaccharide (LPS) to promote dendritic cell (DC) maturation, endowing these cells with Th1-polarizing capacity. Exposure of DCs to Fhmuc in presence of LPS induced enhanced secretion of pro-inflammatory cytokines and expression of co-stimulatory molecules by DCs, promoting their T cell stimulatory capacity and selectively augmenting IFN-3 secretion by allogeneic T cells. Furthermore, exposure of DCs to Fhmuc augmented LPS-induced Toll-like receptor (TLR) 4 expression on the cell surface. Finally, Fhmuc-conditioned DCs induced parasite specific-adaptive immunity with increased levels of IFN-3 secreted by splenocytes from vaccinated animals, and higher parasite-specific IgG antibodies. However, Fhmuc-treated DC conferred modest protection against F. hepatica infection highlighting the potent immuno-regulatory capacity of the parasite. In summary, this work highlights the capacity of a mucin-derived peptide from F. hepatica to enhance LPS-maturation of DCs and induce parasite-specific immune responses with potential implications in vaccination and therapeutic strategies. © The Author(s) 2017. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20452322_v7_n_p_Noya |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
antibody autacoid cytokine gamma interferon glycoprotein p 15095 helminth antibody immunoglobulin enhancer binding protein immunoglobulin G lipopolysaccharide mucin 1 peptide toll like receptor 4 animal biological model C57BL mouse cell polarity cytology dendritic cell drug effect Fasciola hepatica female immunology lymphocyte activation metabolism parasite pathology peritoneal cavity signal transduction species difference spleen Th1 cell vaccination Animals Antibodies Antibodies, Helminth CD11c Antigen Cell Polarity Cytokines Dendritic Cells Fasciola hepatica Female Immunoglobulin G Inflammation Mediators Interferon-gamma Lipopolysaccharides Lymphocyte Activation Mice, Inbred C57BL Models, Biological Mucin-1 NF-kappa B Parasites Peptides Peritoneal Cavity Signal Transduction Species Specificity Spleen Th1 Cells Toll-Like Receptor 4 Vaccination |
| spellingShingle |
antibody autacoid cytokine gamma interferon glycoprotein p 15095 helminth antibody immunoglobulin enhancer binding protein immunoglobulin G lipopolysaccharide mucin 1 peptide toll like receptor 4 animal biological model C57BL mouse cell polarity cytology dendritic cell drug effect Fasciola hepatica female immunology lymphocyte activation metabolism parasite pathology peritoneal cavity signal transduction species difference spleen Th1 cell vaccination Animals Antibodies Antibodies, Helminth CD11c Antigen Cell Polarity Cytokines Dendritic Cells Fasciola hepatica Female Immunoglobulin G Inflammation Mediators Interferon-gamma Lipopolysaccharides Lymphocyte Activation Mice, Inbred C57BL Models, Biological Mucin-1 NF-kappa B Parasites Peptides Peritoneal Cavity Signal Transduction Species Specificity Spleen Th1 Cells Toll-Like Receptor 4 Vaccination Noya, V. Brossard, N. Rodríguez, E. Dergan-Dylon, L.S. Carmona, C. Rabinovich, G.A. Freire, T. A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| topic_facet |
antibody autacoid cytokine gamma interferon glycoprotein p 15095 helminth antibody immunoglobulin enhancer binding protein immunoglobulin G lipopolysaccharide mucin 1 peptide toll like receptor 4 animal biological model C57BL mouse cell polarity cytology dendritic cell drug effect Fasciola hepatica female immunology lymphocyte activation metabolism parasite pathology peritoneal cavity signal transduction species difference spleen Th1 cell vaccination Animals Antibodies Antibodies, Helminth CD11c Antigen Cell Polarity Cytokines Dendritic Cells Fasciola hepatica Female Immunoglobulin G Inflammation Mediators Interferon-gamma Lipopolysaccharides Lymphocyte Activation Mice, Inbred C57BL Models, Biological Mucin-1 NF-kappa B Parasites Peptides Peritoneal Cavity Signal Transduction Species Specificity Spleen Th1 Cells Toll-Like Receptor 4 Vaccination |
| description |
Fasciolosis is a trematode zoonosis of interest in public health and cattle production. We report here the immunostimulatory effect of a 66 mer mucin-like peptide from Fasciola hepatica (Fhmuc), which synergizes with lipopolysaccharide (LPS) to promote dendritic cell (DC) maturation, endowing these cells with Th1-polarizing capacity. Exposure of DCs to Fhmuc in presence of LPS induced enhanced secretion of pro-inflammatory cytokines and expression of co-stimulatory molecules by DCs, promoting their T cell stimulatory capacity and selectively augmenting IFN-3 secretion by allogeneic T cells. Furthermore, exposure of DCs to Fhmuc augmented LPS-induced Toll-like receptor (TLR) 4 expression on the cell surface. Finally, Fhmuc-conditioned DCs induced parasite specific-adaptive immunity with increased levels of IFN-3 secreted by splenocytes from vaccinated animals, and higher parasite-specific IgG antibodies. However, Fhmuc-treated DC conferred modest protection against F. hepatica infection highlighting the potent immuno-regulatory capacity of the parasite. In summary, this work highlights the capacity of a mucin-derived peptide from F. hepatica to enhance LPS-maturation of DCs and induce parasite-specific immune responses with potential implications in vaccination and therapeutic strategies. © The Author(s) 2017. |
| format |
JOUR |
| author |
Noya, V. Brossard, N. Rodríguez, E. Dergan-Dylon, L.S. Carmona, C. Rabinovich, G.A. Freire, T. |
| author_facet |
Noya, V. Brossard, N. Rodríguez, E. Dergan-Dylon, L.S. Carmona, C. Rabinovich, G.A. Freire, T. |
| author_sort |
Noya, V. |
| title |
A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_short |
A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_full |
A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_fullStr |
A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_full_unstemmed |
A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity |
| title_sort |
mucin-like peptide from fasciola hepatica instructs dendritic cells with parasite specific th1-polarizing activity |
| url |
http://hdl.handle.net/20.500.12110/paper_20452322_v7_n_p_Noya |
| work_keys_str_mv |
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