CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Inda |
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todo:paper_20452322_v7_n1_p_Inda2023-10-03T16:38:19Z CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells Inda, C. Bonfiglio, J.J. Dos Santos Claro, P.A. Senin, S.A. Armando, N.G. Deussing, J.M. Silberstein, S. adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR1 in neuronal and neuroendocrine contexts. Here, we show that activated CRHR1 promotes growth arrest and neurite elongation in neuronal hippocampal cells (HT22-CRHR1 cells). By characterising CRHR1 signalling mechanisms involved in the neuritogenic effect, we demonstrate that neurite outgrowth in HT22-CRHR1 cells takes place by a sAC-dependent, ERK1/2-independent signalling cascade. Both tmACs and sAC are involved in corticotropin-releasing hormone (CRH)-mediated CREB phosphorylation and c-fos induction, but only sAC-generated cAMP pools are critical for the neuritogenic effect of CRH, further highlighting the engagement of two sources of cAMP downstream of the activation of a GPCR, and reinforcing the notion that restricted cAMP microdomains may regulate independent cellular processes. © 2017 The Author(s). Fil:Bonfiglio, J.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silberstein, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Inda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone |
| spellingShingle |
adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone Inda, C. Bonfiglio, J.J. Dos Santos Claro, P.A. Senin, S.A. Armando, N.G. Deussing, J.M. Silberstein, S. CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells |
| topic_facet |
adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone |
| description |
Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR1 in neuronal and neuroendocrine contexts. Here, we show that activated CRHR1 promotes growth arrest and neurite elongation in neuronal hippocampal cells (HT22-CRHR1 cells). By characterising CRHR1 signalling mechanisms involved in the neuritogenic effect, we demonstrate that neurite outgrowth in HT22-CRHR1 cells takes place by a sAC-dependent, ERK1/2-independent signalling cascade. Both tmACs and sAC are involved in corticotropin-releasing hormone (CRH)-mediated CREB phosphorylation and c-fos induction, but only sAC-generated cAMP pools are critical for the neuritogenic effect of CRH, further highlighting the engagement of two sources of cAMP downstream of the activation of a GPCR, and reinforcing the notion that restricted cAMP microdomains may regulate independent cellular processes. © 2017 The Author(s). |
| format |
JOUR |
| author |
Inda, C. Bonfiglio, J.J. Dos Santos Claro, P.A. Senin, S.A. Armando, N.G. Deussing, J.M. Silberstein, S. |
| author_facet |
Inda, C. Bonfiglio, J.J. Dos Santos Claro, P.A. Senin, S.A. Armando, N.G. Deussing, J.M. Silberstein, S. |
| author_sort |
Inda, C. |
| title |
CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells |
| title_short |
CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells |
| title_full |
CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells |
| title_fullStr |
CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells |
| title_full_unstemmed |
CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells |
| title_sort |
camp-dependent cell differentiation triggered by activated crhr1 in hippocampal neuronal cells |
| url |
http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Inda |
| work_keys_str_mv |
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| _version_ |
1807315361231536128 |