Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution

The karyotypes of Lucilia cluvia (Walker, 1849) and L. sericata (Meigen, 1826) from Argentina were characterized using conventional staining and the C- and G-like banding techniques. Besides, nucleolus organizer regions (NORs) were detected by fluorescent in situ hybridization (FISH) and silver stai...

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Autores principales: Chirino, M.G., Rossi, L.F., Bressa, M.J., Luaces, J.P., Merani, M.S.
Formato: JOUR
Materias:
Blowflies
G-like banding pattern
Heterochromatin
Karyotype evolution
rDNA-FISH
Sex chromosomes
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19930771_v9_n1_p103_Chirino
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_19930771_v9_n1_p103_Chirino2023-10-03T16:37:33Z Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution Chirino, M.G. Rossi, L.F. Bressa, M.J. Luaces, J.P. Merani, M.S. Blowflies G-like banding pattern Heterochromatin Karyotype evolution rDNA-FISH Sex chromosomes The karyotypes of Lucilia cluvia (Walker, 1849) and L. sericata (Meigen, 1826) from Argentina were characterized using conventional staining and the C- and G-like banding techniques. Besides, nucleolus organizer regions (NORs) were detected by fluorescent in situ hybridization (FISH) and silver staining technique. The chromosome complement of these species comprises five pairs of autosomes and a pair of sex chromosomes (XX/XY, female/male). The autosomes of both species have the same size and morphology, as well as C- and G-like banding patterns. The X and Y chromosomes of L. cluvia are subtelocentric and easily identified due to their very small size. In L. sericata, the X chromosome is metacentric and the largest of the complement, showing a secondary constriction in its short arm, whereas the Y is submetacentric and smaller than the X. The C-banding patterns reflect differences in chromatin structure and composition between the subtelocentric X and Y chromosomes of L. cluvia and the biarmed sex chromosomes of L. sericata. These differences in the sex chromosomes may be due to distinct amounts of constitutive heterochromatin. In L. cluvia, the NORs are placed at one end of the long-X and of the long-Y chromosome arms, whereas one of the NORs is disposed in the secondary constriction of the short-X chromosome arm and the other on the long-Y chromosome arm in L. sericata. Although the G-like banding technique does not yield G-bands like those in mammalian chromosomes, it shows a high degree chromosomal homology in both species because each pair of autosomes was correctly paired. This chromosome similarity suggests the absence of autosomal rearrangements during karyotype evolution in the two species studied. Fil:Rossi, L.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bressa, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luaces, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19930771_v9_n1_p103_Chirino
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Blowflies
G-like banding pattern
Heterochromatin
Karyotype evolution
rDNA-FISH
Sex chromosomes
spellingShingle Blowflies
G-like banding pattern
Heterochromatin
Karyotype evolution
rDNA-FISH
Sex chromosomes
Chirino, M.G.
Rossi, L.F.
Bressa, M.J.
Luaces, J.P.
Merani, M.S.
Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution
topic_facet Blowflies
G-like banding pattern
Heterochromatin
Karyotype evolution
rDNA-FISH
Sex chromosomes
description The karyotypes of Lucilia cluvia (Walker, 1849) and L. sericata (Meigen, 1826) from Argentina were characterized using conventional staining and the C- and G-like banding techniques. Besides, nucleolus organizer regions (NORs) were detected by fluorescent in situ hybridization (FISH) and silver staining technique. The chromosome complement of these species comprises five pairs of autosomes and a pair of sex chromosomes (XX/XY, female/male). The autosomes of both species have the same size and morphology, as well as C- and G-like banding patterns. The X and Y chromosomes of L. cluvia are subtelocentric and easily identified due to their very small size. In L. sericata, the X chromosome is metacentric and the largest of the complement, showing a secondary constriction in its short arm, whereas the Y is submetacentric and smaller than the X. The C-banding patterns reflect differences in chromatin structure and composition between the subtelocentric X and Y chromosomes of L. cluvia and the biarmed sex chromosomes of L. sericata. These differences in the sex chromosomes may be due to distinct amounts of constitutive heterochromatin. In L. cluvia, the NORs are placed at one end of the long-X and of the long-Y chromosome arms, whereas one of the NORs is disposed in the secondary constriction of the short-X chromosome arm and the other on the long-Y chromosome arm in L. sericata. Although the G-like banding technique does not yield G-bands like those in mammalian chromosomes, it shows a high degree chromosomal homology in both species because each pair of autosomes was correctly paired. This chromosome similarity suggests the absence of autosomal rearrangements during karyotype evolution in the two species studied.
format JOUR
author Chirino, M.G.
Rossi, L.F.
Bressa, M.J.
Luaces, J.P.
Merani, M.S.
author_facet Chirino, M.G.
Rossi, L.F.
Bressa, M.J.
Luaces, J.P.
Merani, M.S.
author_sort Chirino, M.G.
title Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution
title_short Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution
title_full Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution
title_fullStr Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution
title_full_unstemmed Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution
title_sort comparative study of mitotic chromosomes in two blowflies, lucilia sericata and l. cluvia (diptera, calliphoridae), by c- and g-like banding patterns and rrna loci, and implications for karyotype evolution
url http://hdl.handle.net/20.500.12110/paper_19930771_v9_n1_p103_Chirino
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