Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast

Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer e...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Fletcher, S.J., Hapon, M.B., Callegari, E.A., Crosbie, M.L., Santiso, N., Ursino, A., Amato, A.R., Gutiérrez, A., Sacca, P.A., Dreszman, R., Pérez, A., Carón, R.W., Calvo, J.C., Pistone-Creydt, V.
Formato: JOUR
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19492553_v9_n57_p31007_Fletcher
Aporte de:
id todo:paper_19492553_v9_n57_p31007_Fletcher
record_format dspace
spelling todo:paper_19492553_v9_n57_p31007_Fletcher2023-10-03T16:37:20Z Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast Fletcher, S.J. Hapon, M.B. Callegari, E.A. Crosbie, M.L. Santiso, N. Ursino, A. Amato, A.R. Gutiérrez, A. Sacca, P.A. Dreszman, R. Pérez, A. Carón, R.W. Calvo, J.C. Pistone-Creydt, V. Adipose tissue Epithelial-stromal interaction Human breast cancer Proteomics analysis Tumor microenvironment angiogenin apolipoprotein A1 apolipoprotein A2 complement component C3 CXCL1 chemokine CXCL2 chemokine CXCL3 chemokine desmin interleukin 6 interleukin 8 monocyte chemotactic protein 1 monocyte chemotactic protein 2 RANTES vimentin adipose tissue Article body mass breast cancer cancer grading cancer staging cell communication complement activation controlled study correlation analysis cytokine release explant histopathology human human tissue immune response liquid chromatography-mass spectrometry metabolism molecular biology polyacrylamide gel electrophoresis protein analysis protein function proteomics signal transduction Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer epithelial cells when compared to human adipose tissue from normal breasts (hATN). In this work, we aim to identify specific proteins and molecular/biological pathways associated with the secretion profile of hATT and hATN explants. hATT-CMs and hATN-CMs were separated by SDS-PAGE and analyzed by means of two-dimensional nano-liquid chromatography-mass spectrometry. The data was analyzed using ProteoIQ and FunRich software. In addition, 42 cytokines from hATTCMs and hATN-CMs were assayed by a protein antibody assay. Compared to hATNCMs, hATT-CMs showed greater protein diversity. We found that hATT-CMs presented a greater amount of proteins related to complement system activity, metabolism and immune system, as well as proteins involved in a variety of biological processes such as signal transduction and cell communication. Specifically, apolipoprotein AI and AII, complement component 3, and vimentin and desmin were significantly increased in hATT-CMs versus hATN-CMs. Moreover, a multivariate discriminant analysis of the cytokines detected by the array showed that IL-6, MCP-2 and GRO cytokines were sufficient and necessary to differentiate hATT-CMs from hATN-CMs. This analysis also showed that the levels of these three cytokines, taken together, correlated with stage and histological grade of the tumor in the hATT-CMs group, and with body mass index in the hATN-CMs group. © Fletcher et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19492553_v9_n57_p31007_Fletcher
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Adipose tissue
Epithelial-stromal interaction
Human breast cancer
Proteomics analysis
Tumor microenvironment
angiogenin
apolipoprotein A1
apolipoprotein A2
complement component C3
CXCL1 chemokine
CXCL2 chemokine
CXCL3 chemokine
desmin
interleukin 6
interleukin 8
monocyte chemotactic protein 1
monocyte chemotactic protein 2
RANTES
vimentin
adipose tissue
Article
body mass
breast cancer
cancer grading
cancer staging
cell communication
complement activation
controlled study
correlation analysis
cytokine release
explant
histopathology
human
human tissue
immune response
liquid chromatography-mass spectrometry
metabolism
molecular biology
polyacrylamide gel electrophoresis
protein analysis
protein function
proteomics
signal transduction
spellingShingle Adipose tissue
Epithelial-stromal interaction
Human breast cancer
Proteomics analysis
Tumor microenvironment
angiogenin
apolipoprotein A1
apolipoprotein A2
complement component C3
CXCL1 chemokine
CXCL2 chemokine
CXCL3 chemokine
desmin
interleukin 6
interleukin 8
monocyte chemotactic protein 1
monocyte chemotactic protein 2
RANTES
vimentin
adipose tissue
Article
body mass
breast cancer
cancer grading
cancer staging
cell communication
complement activation
controlled study
correlation analysis
cytokine release
explant
histopathology
human
human tissue
immune response
liquid chromatography-mass spectrometry
metabolism
molecular biology
polyacrylamide gel electrophoresis
protein analysis
protein function
proteomics
signal transduction
Fletcher, S.J.
Hapon, M.B.
Callegari, E.A.
Crosbie, M.L.
Santiso, N.
Ursino, A.
Amato, A.R.
Gutiérrez, A.
Sacca, P.A.
Dreszman, R.
Pérez, A.
Carón, R.W.
Calvo, J.C.
Pistone-Creydt, V.
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast
topic_facet Adipose tissue
Epithelial-stromal interaction
Human breast cancer
Proteomics analysis
Tumor microenvironment
angiogenin
apolipoprotein A1
apolipoprotein A2
complement component C3
CXCL1 chemokine
CXCL2 chemokine
CXCL3 chemokine
desmin
interleukin 6
interleukin 8
monocyte chemotactic protein 1
monocyte chemotactic protein 2
RANTES
vimentin
adipose tissue
Article
body mass
breast cancer
cancer grading
cancer staging
cell communication
complement activation
controlled study
correlation analysis
cytokine release
explant
histopathology
human
human tissue
immune response
liquid chromatography-mass spectrometry
metabolism
molecular biology
polyacrylamide gel electrophoresis
protein analysis
protein function
proteomics
signal transduction
description Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer epithelial cells when compared to human adipose tissue from normal breasts (hATN). In this work, we aim to identify specific proteins and molecular/biological pathways associated with the secretion profile of hATT and hATN explants. hATT-CMs and hATN-CMs were separated by SDS-PAGE and analyzed by means of two-dimensional nano-liquid chromatography-mass spectrometry. The data was analyzed using ProteoIQ and FunRich software. In addition, 42 cytokines from hATTCMs and hATN-CMs were assayed by a protein antibody assay. Compared to hATNCMs, hATT-CMs showed greater protein diversity. We found that hATT-CMs presented a greater amount of proteins related to complement system activity, metabolism and immune system, as well as proteins involved in a variety of biological processes such as signal transduction and cell communication. Specifically, apolipoprotein AI and AII, complement component 3, and vimentin and desmin were significantly increased in hATT-CMs versus hATN-CMs. Moreover, a multivariate discriminant analysis of the cytokines detected by the array showed that IL-6, MCP-2 and GRO cytokines were sufficient and necessary to differentiate hATT-CMs from hATN-CMs. This analysis also showed that the levels of these three cytokines, taken together, correlated with stage and histological grade of the tumor in the hATT-CMs group, and with body mass index in the hATN-CMs group. © Fletcher et al.
format JOUR
author Fletcher, S.J.
Hapon, M.B.
Callegari, E.A.
Crosbie, M.L.
Santiso, N.
Ursino, A.
Amato, A.R.
Gutiérrez, A.
Sacca, P.A.
Dreszman, R.
Pérez, A.
Carón, R.W.
Calvo, J.C.
Pistone-Creydt, V.
author_facet Fletcher, S.J.
Hapon, M.B.
Callegari, E.A.
Crosbie, M.L.
Santiso, N.
Ursino, A.
Amato, A.R.
Gutiérrez, A.
Sacca, P.A.
Dreszman, R.
Pérez, A.
Carón, R.W.
Calvo, J.C.
Pistone-Creydt, V.
author_sort Fletcher, S.J.
title Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast
title_short Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast
title_full Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast
title_fullStr Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast
title_full_unstemmed Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast
title_sort comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast
url http://hdl.handle.net/20.500.12110/paper_19492553_v9_n57_p31007_Fletcher
work_keys_str_mv AT fletchersj comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT haponmb comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT callegariea comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT crosbieml comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT santison comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT ursinoa comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT amatoar comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT gutierreza comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT saccapa comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT dreszmanr comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT pereza comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT caronrw comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT calvojc comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
AT pistonecreydtv comparativeproteomicsofsolublefactorssecretedbyhumanbreastadiposetissuefromtumorandnormalbreast
_version_ 1782023797980790784