Human renal adipose tissue induces the invasion and progression of renal cell carcinoma
We evaluated the effects of conditioned media (CMs) of human adipose tissue from renal cell carcinoma located near the tumor (hRATnT) or farther away from the tumor (hRATfT), on proliferation, adhesion and migration of tumor (786-O and ACHN) and non-tumor (HK-2) human renal epithelial cell lines. Hu...
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todo:paper_19492553_v8_n55_p94223_CampoVerdeArbocco2023-10-03T16:37:20Z Human renal adipose tissue induces the invasion and progression of renal cell carcinoma Campo-Verde-Arbocco, F. López-Laur, J.D. Romeo, L.R. Giorlando, N. Bruna, F.A. Contador, D.E. López-Fontana, G. Santiano, F.E. Sasso, C.V. Zyla, L.E. López-Fontana, C.M. Calvo, J.C. Carón, R.W. Creydt, V.P. Cancer Epithelial-stromal interactions Human adipose tissue Migration Renal epithelial cells adiponectin AdipoR1 protein AdipoR2 protein leptin mitogen activated protein kinase ObR protein phosphatidylinositol 3 kinase phosphoprotein protein kinase B unclassified drug versican 786-O cell line ACHN cell line adipose tissue Article cancer growth cancer patient cell adhesion cell migration cell proliferation controlled study epithelial cell line HK-2 cell line human human cell human tissue kidney kidney epithelium renal cell carcinoma tumor invasion We evaluated the effects of conditioned media (CMs) of human adipose tissue from renal cell carcinoma located near the tumor (hRATnT) or farther away from the tumor (hRATfT), on proliferation, adhesion and migration of tumor (786-O and ACHN) and non-tumor (HK-2) human renal epithelial cell lines. Human adipose tissues were obtained from patients with renal cell carcinoma (RCC) and CMs from hRATnT and hRATfT incubation. Proliferation, adhesion and migration were quantified in 786-O, ACHN and HK-2 cell lines incubated with hRATnT-, hRATfT- or control-CMs. We evaluated versican, adiponectin and leptin expression in CMs from hRATnT and hRATfT. We evaluated AdipoR1/2, ObR, pERK, pAkt y pPI3K expression on cell lines incubated with CMs. No differences in proliferation of cell lines was found after 24 h of treatment with CMs. All cell lines showed a significant decrease in cell adhesion and increase in cell migration after incubation with hRATnT-CMs vs. hRATfT- or control-CMs. hRATnT-CMs showed increased levels of versican and leptin, compared to hRATfT-CMs. AdipoR2 in 786-O and ACHN cells decreased significantly after incubation with hRATfT- and hRATnT-CMs vs. control-CMs. We observed a decrease in the expression of pAkt in HK-2, 786-O and ACHN incubated with hRATnT-CMs. This result could partially explain the observed changes in migration and cell adhesion. We conclude that hRATnT released factors, such as leptin and versican, could enhance the invasive potential of renal epithelial cell lines and could modulate the progression of the disease. © Campo-Verde-Arbocco et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19492553_v8_n55_p94223_CampoVerdeArbocco |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cancer Epithelial-stromal interactions Human adipose tissue Migration Renal epithelial cells adiponectin AdipoR1 protein AdipoR2 protein leptin mitogen activated protein kinase ObR protein phosphatidylinositol 3 kinase phosphoprotein protein kinase B unclassified drug versican 786-O cell line ACHN cell line adipose tissue Article cancer growth cancer patient cell adhesion cell migration cell proliferation controlled study epithelial cell line HK-2 cell line human human cell human tissue kidney kidney epithelium renal cell carcinoma tumor invasion |
| spellingShingle |
Cancer Epithelial-stromal interactions Human adipose tissue Migration Renal epithelial cells adiponectin AdipoR1 protein AdipoR2 protein leptin mitogen activated protein kinase ObR protein phosphatidylinositol 3 kinase phosphoprotein protein kinase B unclassified drug versican 786-O cell line ACHN cell line adipose tissue Article cancer growth cancer patient cell adhesion cell migration cell proliferation controlled study epithelial cell line HK-2 cell line human human cell human tissue kidney kidney epithelium renal cell carcinoma tumor invasion Campo-Verde-Arbocco, F. López-Laur, J.D. Romeo, L.R. Giorlando, N. Bruna, F.A. Contador, D.E. López-Fontana, G. Santiano, F.E. Sasso, C.V. Zyla, L.E. López-Fontana, C.M. Calvo, J.C. Carón, R.W. Creydt, V.P. Human renal adipose tissue induces the invasion and progression of renal cell carcinoma |
| topic_facet |
Cancer Epithelial-stromal interactions Human adipose tissue Migration Renal epithelial cells adiponectin AdipoR1 protein AdipoR2 protein leptin mitogen activated protein kinase ObR protein phosphatidylinositol 3 kinase phosphoprotein protein kinase B unclassified drug versican 786-O cell line ACHN cell line adipose tissue Article cancer growth cancer patient cell adhesion cell migration cell proliferation controlled study epithelial cell line HK-2 cell line human human cell human tissue kidney kidney epithelium renal cell carcinoma tumor invasion |
| description |
We evaluated the effects of conditioned media (CMs) of human adipose tissue from renal cell carcinoma located near the tumor (hRATnT) or farther away from the tumor (hRATfT), on proliferation, adhesion and migration of tumor (786-O and ACHN) and non-tumor (HK-2) human renal epithelial cell lines. Human adipose tissues were obtained from patients with renal cell carcinoma (RCC) and CMs from hRATnT and hRATfT incubation. Proliferation, adhesion and migration were quantified in 786-O, ACHN and HK-2 cell lines incubated with hRATnT-, hRATfT- or control-CMs. We evaluated versican, adiponectin and leptin expression in CMs from hRATnT and hRATfT. We evaluated AdipoR1/2, ObR, pERK, pAkt y pPI3K expression on cell lines incubated with CMs. No differences in proliferation of cell lines was found after 24 h of treatment with CMs. All cell lines showed a significant decrease in cell adhesion and increase in cell migration after incubation with hRATnT-CMs vs. hRATfT- or control-CMs. hRATnT-CMs showed increased levels of versican and leptin, compared to hRATfT-CMs. AdipoR2 in 786-O and ACHN cells decreased significantly after incubation with hRATfT- and hRATnT-CMs vs. control-CMs. We observed a decrease in the expression of pAkt in HK-2, 786-O and ACHN incubated with hRATnT-CMs. This result could partially explain the observed changes in migration and cell adhesion. We conclude that hRATnT released factors, such as leptin and versican, could enhance the invasive potential of renal epithelial cell lines and could modulate the progression of the disease. © Campo-Verde-Arbocco et al. |
| format |
JOUR |
| author |
Campo-Verde-Arbocco, F. López-Laur, J.D. Romeo, L.R. Giorlando, N. Bruna, F.A. Contador, D.E. López-Fontana, G. Santiano, F.E. Sasso, C.V. Zyla, L.E. López-Fontana, C.M. Calvo, J.C. Carón, R.W. Creydt, V.P. |
| author_facet |
Campo-Verde-Arbocco, F. López-Laur, J.D. Romeo, L.R. Giorlando, N. Bruna, F.A. Contador, D.E. López-Fontana, G. Santiano, F.E. Sasso, C.V. Zyla, L.E. López-Fontana, C.M. Calvo, J.C. Carón, R.W. Creydt, V.P. |
| author_sort |
Campo-Verde-Arbocco, F. |
| title |
Human renal adipose tissue induces the invasion and progression of renal cell carcinoma |
| title_short |
Human renal adipose tissue induces the invasion and progression of renal cell carcinoma |
| title_full |
Human renal adipose tissue induces the invasion and progression of renal cell carcinoma |
| title_fullStr |
Human renal adipose tissue induces the invasion and progression of renal cell carcinoma |
| title_full_unstemmed |
Human renal adipose tissue induces the invasion and progression of renal cell carcinoma |
| title_sort |
human renal adipose tissue induces the invasion and progression of renal cell carcinoma |
| url |
http://hdl.handle.net/20.500.12110/paper_19492553_v8_n55_p94223_CampoVerdeArbocco |
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