Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli
Growth and polymer synthesis were studied in a recombinant E. coli strain carrying phaBAC and phaP of Azotobacter sp. strain FA8 using different carbon sources and oxygen availability conditions. The results obtained with glucose or glycerol were completely different, demonstrating that the metaboli...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_19491018_v1_n4_p291_Nikel |
| Aporte de: |
| id |
todo:paper_19491018_v1_n4_p291_Nikel |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_19491018_v1_n4_p291_Nikel2023-10-03T16:37:14Z Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli Nikel, P.I. de Almeida, A. Giordano, A.M. Pettinari, M.J. Aeration Carbon partitioning Escherichia coli Glucose Glycerol Oxygen availability Poly(3-hydroxybutyrate) glycerol poly(3 hydroxybutyric acid) aeration article bacterial growth bacterial metabolism bacterium culture carbon source Escherichia coli nonhuman oxidation reduction reaction synthesis Carbon Escherichia coli Glucose Glycerol Hydroxybutyrates Oxidation-Reduction Oxygen Polyesters Azotobacter Escherichia coli Growth and polymer synthesis were studied in a recombinant E. coli strain carrying phaBAC and phaP of Azotobacter sp. strain FA8 using different carbon sources and oxygen availability conditions. The results obtained with glucose or glycerol were completely different, demonstrating that the metabolic routes leading to the synthesis of the polymer when using glycerol do not respond to environmental conditions such as oxygen availability in the same way as they do when other substrates, such as glucose, are used. When cells were grown in a bioreactor using glucose the amount of polymer accumulated at low aeration was reduced by half when compared to high aeration, while glycerol cultures produced at low aeration almost twice the amount of polymer synthesized at the higher aeration condition. The synthesis of other metabolic products, such as ethanol, lactate, formate and acetate, were also affected by both the carbon source used and aeration conditions. In glucose cultures, lactate and formate production increased in low agitation compared to high agitation, while poly(3-hydroxybutyrate) synthesis decreased. In glycerol cultures, the amount of acids produced also increased when agitation was lowered, but carbon flow was mostly redirected towards ethanol and poly(3-hydroxybutyrate). These results indicated that carbon partitioning differed depending on both carbon source and oxygen availability, and that aeration conditions had different effects on the synthesis of the polymer and other metabolic products when glucose or glycerol were used. © 2010 Landes Bioscience. Fil:de Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19491018_v1_n4_p291_Nikel |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aeration Carbon partitioning Escherichia coli Glucose Glycerol Oxygen availability Poly(3-hydroxybutyrate) glycerol poly(3 hydroxybutyric acid) aeration article bacterial growth bacterial metabolism bacterium culture carbon source Escherichia coli nonhuman oxidation reduction reaction synthesis Carbon Escherichia coli Glucose Glycerol Hydroxybutyrates Oxidation-Reduction Oxygen Polyesters Azotobacter Escherichia coli |
| spellingShingle |
Aeration Carbon partitioning Escherichia coli Glucose Glycerol Oxygen availability Poly(3-hydroxybutyrate) glycerol poly(3 hydroxybutyric acid) aeration article bacterial growth bacterial metabolism bacterium culture carbon source Escherichia coli nonhuman oxidation reduction reaction synthesis Carbon Escherichia coli Glucose Glycerol Hydroxybutyrates Oxidation-Reduction Oxygen Polyesters Azotobacter Escherichia coli Nikel, P.I. de Almeida, A. Giordano, A.M. Pettinari, M.J. Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli |
| topic_facet |
Aeration Carbon partitioning Escherichia coli Glucose Glycerol Oxygen availability Poly(3-hydroxybutyrate) glycerol poly(3 hydroxybutyric acid) aeration article bacterial growth bacterial metabolism bacterium culture carbon source Escherichia coli nonhuman oxidation reduction reaction synthesis Carbon Escherichia coli Glucose Glycerol Hydroxybutyrates Oxidation-Reduction Oxygen Polyesters Azotobacter Escherichia coli |
| description |
Growth and polymer synthesis were studied in a recombinant E. coli strain carrying phaBAC and phaP of Azotobacter sp. strain FA8 using different carbon sources and oxygen availability conditions. The results obtained with glucose or glycerol were completely different, demonstrating that the metabolic routes leading to the synthesis of the polymer when using glycerol do not respond to environmental conditions such as oxygen availability in the same way as they do when other substrates, such as glucose, are used. When cells were grown in a bioreactor using glucose the amount of polymer accumulated at low aeration was reduced by half when compared to high aeration, while glycerol cultures produced at low aeration almost twice the amount of polymer synthesized at the higher aeration condition. The synthesis of other metabolic products, such as ethanol, lactate, formate and acetate, were also affected by both the carbon source used and aeration conditions. In glucose cultures, lactate and formate production increased in low agitation compared to high agitation, while poly(3-hydroxybutyrate) synthesis decreased. In glycerol cultures, the amount of acids produced also increased when agitation was lowered, but carbon flow was mostly redirected towards ethanol and poly(3-hydroxybutyrate). These results indicated that carbon partitioning differed depending on both carbon source and oxygen availability, and that aeration conditions had different effects on the synthesis of the polymer and other metabolic products when glucose or glycerol were used. © 2010 Landes Bioscience. |
| format |
JOUR |
| author |
Nikel, P.I. de Almeida, A. Giordano, A.M. Pettinari, M.J. |
| author_facet |
Nikel, P.I. de Almeida, A. Giordano, A.M. Pettinari, M.J. |
| author_sort |
Nikel, P.I. |
| title |
Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli |
| title_short |
Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli |
| title_full |
Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli |
| title_fullStr |
Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli |
| title_full_unstemmed |
Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli |
| title_sort |
redox driven metabolic tuning: carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in escherichia coli |
| url |
http://hdl.handle.net/20.500.12110/paper_19491018_v1_n4_p291_Nikel |
| work_keys_str_mv |
AT nikelpi redoxdrivenmetabolictuningcarbonsourceandaerationaffectsynthesisofpoly3hydroxybutyrateinescherichiacoli AT dealmeidaa redoxdrivenmetabolictuningcarbonsourceandaerationaffectsynthesisofpoly3hydroxybutyrateinescherichiacoli AT giordanoam redoxdrivenmetabolictuningcarbonsourceandaerationaffectsynthesisofpoly3hydroxybutyrateinescherichiacoli AT pettinarimj redoxdrivenmetabolictuningcarbonsourceandaerationaffectsynthesisofpoly3hydroxybutyrateinescherichiacoli |
| _version_ |
1782025042773671936 |