Smaug variants in neural and non-neuronal cells

Mammalian Smaug1/Samd4a is an mRNA regulator involved in synapse plasticity and additional non-neuronal functions. Here we analyzed the expression of Smaug1/Samd4a variants and Smaug2/Samd4b in primary hippocampal neurons and non-neuronal cell lines. We found that multiple Smaug proteins are present...

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Autores principales: Fernández-Alvarez, A.J., Pascual, M.L., Boccaccio, G.L., Thomas, M.G.
Formato: JOUR
Materias:
mRNA silencing
Muscular dystrophy
Processing bodies
Stress granules
Synaptogenesis
Translation
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19420889_v9_n2_p1_FernandezAlvarez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_19420889_v9_n2_p1_FernandezAlvarez2023-10-03T16:36:56Z Smaug variants in neural and non-neuronal cells Fernández-Alvarez, A.J. Pascual, M.L. Boccaccio, G.L. Thomas, M.G. mRNA silencing Muscular dystrophy Processing bodies Stress granules Synaptogenesis Translation Mammalian Smaug1/Samd4a is an mRNA regulator involved in synapse plasticity and additional non-neuronal functions. Here we analyzed the expression of Smaug1/Samd4a variants and Smaug2/Samd4b in primary hippocampal neurons and non-neuronal cell lines. We found that multiple Smaug proteins are present in several mammalian cell lines, including a canonical full length Smaug1, a Smaug1 variant that lacks the third exon, termed DEIII, and Smaug2, the product of a highly homologous gene. These three major isoforms are expressed differentially along neuron development and form cytosolic bodies when transfected in cell lines. By using luciferase reporters, we found that the DEIII isoform, which lacks 10 amino acids in the sterile α motif involved in RNA binding, shows a RNA-binding capacity and repressor activity comparable to that of the full length Smaug1. These observations are an important groundwork for molecular studies of the Smaug posttranscriptional pathway, which is relevant to neuron development, mitochondrial function and muscle physiology in health and disease. © 2016 Ana Julia Fernández-Alvarez, Malena Lucía Pascual, Graciela Lidia Boccaccio, and María Gabriela Thomas. Fil:Boccaccio, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Thomas, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19420889_v9_n2_p1_FernandezAlvarez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic mRNA silencing
Muscular dystrophy
Processing bodies
Stress granules
Synaptogenesis
Translation
spellingShingle mRNA silencing
Muscular dystrophy
Processing bodies
Stress granules
Synaptogenesis
Translation
Fernández-Alvarez, A.J.
Pascual, M.L.
Boccaccio, G.L.
Thomas, M.G.
Smaug variants in neural and non-neuronal cells
topic_facet mRNA silencing
Muscular dystrophy
Processing bodies
Stress granules
Synaptogenesis
Translation
description Mammalian Smaug1/Samd4a is an mRNA regulator involved in synapse plasticity and additional non-neuronal functions. Here we analyzed the expression of Smaug1/Samd4a variants and Smaug2/Samd4b in primary hippocampal neurons and non-neuronal cell lines. We found that multiple Smaug proteins are present in several mammalian cell lines, including a canonical full length Smaug1, a Smaug1 variant that lacks the third exon, termed DEIII, and Smaug2, the product of a highly homologous gene. These three major isoforms are expressed differentially along neuron development and form cytosolic bodies when transfected in cell lines. By using luciferase reporters, we found that the DEIII isoform, which lacks 10 amino acids in the sterile α motif involved in RNA binding, shows a RNA-binding capacity and repressor activity comparable to that of the full length Smaug1. These observations are an important groundwork for molecular studies of the Smaug posttranscriptional pathway, which is relevant to neuron development, mitochondrial function and muscle physiology in health and disease. © 2016 Ana Julia Fernández-Alvarez, Malena Lucía Pascual, Graciela Lidia Boccaccio, and María Gabriela Thomas.
format JOUR
author Fernández-Alvarez, A.J.
Pascual, M.L.
Boccaccio, G.L.
Thomas, M.G.
author_facet Fernández-Alvarez, A.J.
Pascual, M.L.
Boccaccio, G.L.
Thomas, M.G.
author_sort Fernández-Alvarez, A.J.
title Smaug variants in neural and non-neuronal cells
title_short Smaug variants in neural and non-neuronal cells
title_full Smaug variants in neural and non-neuronal cells
title_fullStr Smaug variants in neural and non-neuronal cells
title_full_unstemmed Smaug variants in neural and non-neuronal cells
title_sort smaug variants in neural and non-neuronal cells
url http://hdl.handle.net/20.500.12110/paper_19420889_v9_n2_p1_FernandezAlvarez
work_keys_str_mv AT fernandezalvarezaj smaugvariantsinneuralandnonneuronalcells
AT pascualml smaugvariantsinneuralandnonneuronalcells
AT boccacciogl smaugvariantsinneuralandnonneuronalcells
AT thomasmg smaugvariantsinneuralandnonneuronalcells
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