Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance
The internal transcribed spacer region (ITS1 and ITS2) of the 18S-25S nuclear ribosomal DNA sequence and the intervening 5.8S region from five species of the genus Oxalis was amplified by polymerase chain reaction and subjected to direct DN A sequencing. On the basis of cytogenetic studies some spec...
Guardado en:
| Autores principales: | , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_19401736_v6_n6_p361_Tosto |
| Aporte de: |
| id |
todo:paper_19401736_v6_n6_p361_Tosto |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_19401736_v6_n6_p361_Tosto2023-10-03T16:36:53Z Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance Tosto, D.S. Hopp, H.E. Andean crops ITS Oxalis rDNA plant DNA ribosome DNA article genetic transcription genetics molecular genetics nucleotide sequence plant sequence homology Base Sequence DNA, Plant DNA, Ribosomal Molecular Sequence Data Plants Sequence Homology, Nucleic Acid Transcription, Genetic Oxalis Oxalis tuberosa Tuberosa The internal transcribed spacer region (ITS1 and ITS2) of the 18S-25S nuclear ribosomal DNA sequence and the intervening 5.8S region from five species of the genus Oxalis was amplified by polymerase chain reaction and subjected to direct DN A sequencing. On the basis of cytogenetic studies some species of this genus were postulated to be related by the number of chromosomes. Sequence homologies in the ITS1, 5.8S and ITS2 among species are in good agreement with previous relationships established on the basis of chromosome numbers. We also indemnified a highly conserved sequence of six bp in the ITS1, reported to be present in a wide range of flowering plants, but not in the Oxalidaceae family to which the genus Oxalis belongs to. © 1996 Informa UK Ltd All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19401736_v6_n6_p361_Tosto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Andean crops ITS Oxalis rDNA plant DNA ribosome DNA article genetic transcription genetics molecular genetics nucleotide sequence plant sequence homology Base Sequence DNA, Plant DNA, Ribosomal Molecular Sequence Data Plants Sequence Homology, Nucleic Acid Transcription, Genetic Oxalis Oxalis tuberosa Tuberosa |
| spellingShingle |
Andean crops ITS Oxalis rDNA plant DNA ribosome DNA article genetic transcription genetics molecular genetics nucleotide sequence plant sequence homology Base Sequence DNA, Plant DNA, Ribosomal Molecular Sequence Data Plants Sequence Homology, Nucleic Acid Transcription, Genetic Oxalis Oxalis tuberosa Tuberosa Tosto, D.S. Hopp, H.E. Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance |
| topic_facet |
Andean crops ITS Oxalis rDNA plant DNA ribosome DNA article genetic transcription genetics molecular genetics nucleotide sequence plant sequence homology Base Sequence DNA, Plant DNA, Ribosomal Molecular Sequence Data Plants Sequence Homology, Nucleic Acid Transcription, Genetic Oxalis Oxalis tuberosa Tuberosa |
| description |
The internal transcribed spacer region (ITS1 and ITS2) of the 18S-25S nuclear ribosomal DNA sequence and the intervening 5.8S region from five species of the genus Oxalis was amplified by polymerase chain reaction and subjected to direct DN A sequencing. On the basis of cytogenetic studies some species of this genus were postulated to be related by the number of chromosomes. Sequence homologies in the ITS1, 5.8S and ITS2 among species are in good agreement with previous relationships established on the basis of chromosome numbers. We also indemnified a highly conserved sequence of six bp in the ITS1, reported to be present in a wide range of flowering plants, but not in the Oxalidaceae family to which the genus Oxalis belongs to. © 1996 Informa UK Ltd All rights reserved. |
| format |
JOUR |
| author |
Tosto, D.S. Hopp, H.E. |
| author_facet |
Tosto, D.S. Hopp, H.E. |
| author_sort |
Tosto, D.S. |
| title |
Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance |
| title_short |
Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance |
| title_full |
Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance |
| title_fullStr |
Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance |
| title_full_unstemmed |
Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance |
| title_sort |
sequence analysis of the 5.8s ribosomal dna and internal transcribed spacers (its1 and its2) from five species of the oxalis tuberosa alliance |
| url |
http://hdl.handle.net/20.500.12110/paper_19401736_v6_n6_p361_Tosto |
| work_keys_str_mv |
AT tostods sequenceanalysisofthe58sribosomaldnaandinternaltranscribedspacersits1andits2fromfivespeciesoftheoxalistuberosaalliance AT hopphe sequenceanalysisofthe58sribosomaldnaandinternaltranscribedspacersits1andits2fromfivespeciesoftheoxalistuberosaalliance |
| _version_ |
1807322782004936704 |