Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami
We study the distance-dependent quenching of fluorescence due to a metallic nanoparticle in proximity of a fluorophore. In our single-molecule measurements, we achieve excellent control over structure and stoichiometry by using self-assembled DNA structures (DNA origami) as a breadboard where both t...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_19360851_v6_n4_p3189_Acuna |
| Aporte de: |
| id |
todo:paper_19360851_v6_n4_p3189_Acuna |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_19360851_v6_n4_p3189_Acuna2023-10-03T16:36:40Z Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami Acuna, G.P. Bucher, M. Stein, I.H. Steinhauer, C. Kuzyk, A. Holzmeister, P. Schreiber, R. Moroz, A. Stefani, F.D. Liedl, T. Simmel, F.C. Tinnefeld, P. DNA origami DNA self-assembly fluorescence quenching gold nanoparticles single-molecule fluorescence Dna origamis DNA self-assembly Fluorescence quenching Gold Nanoparticles Single-molecule DNA Energy transfer Fluorescence Metal nanoparticles Molecules Quenching Spectroscopic analysis Stoichiometry Fluorophores DNA fluorescent dye gold metal nanoparticle article chemistry methodology nanotechnology optics spectrofluorometry DNA Fluorescent Dyes Gold Metal Nanoparticles Nanotechnology Optics and Photonics Spectrometry, Fluorescence We study the distance-dependent quenching of fluorescence due to a metallic nanoparticle in proximity of a fluorophore. In our single-molecule measurements, we achieve excellent control over structure and stoichiometry by using self-assembled DNA structures (DNA origami) as a breadboard where both the fluorophore and the 10 nm metallic nanoparticle are positioned with nanometer precision. The single-molecule spectroscopy method employed here reports on the co-localization of particle and dye, while fluorescence lifetime imaging is used to directly obtain the correlation of intensity and fluorescence lifetime for varying particle to dye distances. Our data can be well explained by exact calculations that include dipole-dipole orientation and distances. Fitting with a more practical model for nanosurface energy transfer yields 10.4 nm as the characteristic distance of 50% energy transfer. The use of DNA nanotechnology together with minimal sample usage by attaching the particles to the DNA origami directly on the microscope coverslip paves the way for more complex experiments exploiting dye-nanoparticle interactions. © 2012 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19360851_v6_n4_p3189_Acuna |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
DNA origami DNA self-assembly fluorescence quenching gold nanoparticles single-molecule fluorescence Dna origamis DNA self-assembly Fluorescence quenching Gold Nanoparticles Single-molecule DNA Energy transfer Fluorescence Metal nanoparticles Molecules Quenching Spectroscopic analysis Stoichiometry Fluorophores DNA fluorescent dye gold metal nanoparticle article chemistry methodology nanotechnology optics spectrofluorometry DNA Fluorescent Dyes Gold Metal Nanoparticles Nanotechnology Optics and Photonics Spectrometry, Fluorescence |
| spellingShingle |
DNA origami DNA self-assembly fluorescence quenching gold nanoparticles single-molecule fluorescence Dna origamis DNA self-assembly Fluorescence quenching Gold Nanoparticles Single-molecule DNA Energy transfer Fluorescence Metal nanoparticles Molecules Quenching Spectroscopic analysis Stoichiometry Fluorophores DNA fluorescent dye gold metal nanoparticle article chemistry methodology nanotechnology optics spectrofluorometry DNA Fluorescent Dyes Gold Metal Nanoparticles Nanotechnology Optics and Photonics Spectrometry, Fluorescence Acuna, G.P. Bucher, M. Stein, I.H. Steinhauer, C. Kuzyk, A. Holzmeister, P. Schreiber, R. Moroz, A. Stefani, F.D. Liedl, T. Simmel, F.C. Tinnefeld, P. Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami |
| topic_facet |
DNA origami DNA self-assembly fluorescence quenching gold nanoparticles single-molecule fluorescence Dna origamis DNA self-assembly Fluorescence quenching Gold Nanoparticles Single-molecule DNA Energy transfer Fluorescence Metal nanoparticles Molecules Quenching Spectroscopic analysis Stoichiometry Fluorophores DNA fluorescent dye gold metal nanoparticle article chemistry methodology nanotechnology optics spectrofluorometry DNA Fluorescent Dyes Gold Metal Nanoparticles Nanotechnology Optics and Photonics Spectrometry, Fluorescence |
| description |
We study the distance-dependent quenching of fluorescence due to a metallic nanoparticle in proximity of a fluorophore. In our single-molecule measurements, we achieve excellent control over structure and stoichiometry by using self-assembled DNA structures (DNA origami) as a breadboard where both the fluorophore and the 10 nm metallic nanoparticle are positioned with nanometer precision. The single-molecule spectroscopy method employed here reports on the co-localization of particle and dye, while fluorescence lifetime imaging is used to directly obtain the correlation of intensity and fluorescence lifetime for varying particle to dye distances. Our data can be well explained by exact calculations that include dipole-dipole orientation and distances. Fitting with a more practical model for nanosurface energy transfer yields 10.4 nm as the characteristic distance of 50% energy transfer. The use of DNA nanotechnology together with minimal sample usage by attaching the particles to the DNA origami directly on the microscope coverslip paves the way for more complex experiments exploiting dye-nanoparticle interactions. © 2012 American Chemical Society. |
| format |
JOUR |
| author |
Acuna, G.P. Bucher, M. Stein, I.H. Steinhauer, C. Kuzyk, A. Holzmeister, P. Schreiber, R. Moroz, A. Stefani, F.D. Liedl, T. Simmel, F.C. Tinnefeld, P. |
| author_facet |
Acuna, G.P. Bucher, M. Stein, I.H. Steinhauer, C. Kuzyk, A. Holzmeister, P. Schreiber, R. Moroz, A. Stefani, F.D. Liedl, T. Simmel, F.C. Tinnefeld, P. |
| author_sort |
Acuna, G.P. |
| title |
Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami |
| title_short |
Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami |
| title_full |
Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami |
| title_fullStr |
Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami |
| title_full_unstemmed |
Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami |
| title_sort |
distance dependence of single-fluorophore quenching by gold nanoparticles studied on dna origami |
| url |
http://hdl.handle.net/20.500.12110/paper_19360851_v6_n4_p3189_Acuna |
| work_keys_str_mv |
AT acunagp distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT bucherm distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT steinih distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT steinhauerc distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT kuzyka distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT holzmeisterp distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT schreiberr distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT moroza distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT stefanifd distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT liedlt distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT simmelfc distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami AT tinnefeldp distancedependenceofsinglefluorophorequenchingbygoldnanoparticlesstudiedondnaorigami |
| _version_ |
1807324555243421696 |