Rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under Foehn-induced dry wind
Foehn-like extreme hot and dry wind conditions (34 °C, >2.5 kPa vapor pressure deficit, and 7 m s-1) strongly affect grain quality in rice (Oryza sativa L.). This is a current concern because of the increasing frequency and intensity of combined heat and water-deficit stress under climate cha...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_19326203_v9_n10_p_Wada |
| Aporte de: |
| id |
todo:paper_19326203_v9_n10_p_Wada |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_19326203_v9_n10_p_Wada2023-10-03T16:35:39Z Rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under Foehn-induced dry wind Wada, H. Masumoto-Kubo, C. Gholipour, Y. Nonami, H. Tanaka, F. Erra-Balsells, R. Tsutsumi, K. Hiraoka, K. Morita, S. carbon 13 starch starch water Article biosynthesis carbohydrate synthesis cell metabolism controlled study degradation kinetics down regulation dry wind electrospray gene expression growth regulation mass spectrometer nonhuman photosynthesis physical chemistry plant osmotic adjustment plant water potential plant water relation polymerase chain reaction rice seed kernel temperature sensitivity wind anatomy and histology drug effects endosperm gene expression regulation genetics heat metabolism organ size Oryza osmosis time factor Endosperm Gene Expression Regulation, Plant Hot Temperature Organ Size Oryza Osmosis Photosynthesis Starch Time Factors Water Wind Foehn-like extreme hot and dry wind conditions (34 °C, >2.5 kPa vapor pressure deficit, and 7 m s-1) strongly affect grain quality in rice (Oryza sativa L.). This is a current concern because of the increasing frequency and intensity of combined heat and water-deficit stress under climate change. Foehn-induced dry wind conditions during the grain-filling stage increase ring-shaped chalkiness as a result of spatiotemporal reduction in starch accumulation in the endosperm, but kernel growth is sometimes maintained by osmotic adjustment. Here, we assess the effects of dry wind on chalky ring formation in environmentally controlled growth chambers. Our results showed that hot and dry wind conditions that lasted for > 24 h dramatically increased chalky ring formation. Hot and dry wind conditions temporarily reduced panicle water potential to - 0.65 MPa; however, kernel growth was maintained by osmotic adjustment at control levels with increased transport of assimilate to the growing kernels. Dynamic tracer analysis with a nano-electrospray-ionization Orbitrap mass spectrometer and quantitative polymerase chain reaction analysis revealed that starch degradation was negligible in the short-term treatment. Overall expression of starch synthesis-related genes was found to be down-regulated at moderately low water potential. Because the events observed at low water potential preceded the packing of starch granules in cells, we concluded that reduced rates of starch biosynthesis play a central role in the events of cellular metabolism that are altered at osmotic adjustment, which leads to chalky ring formation under short-term hot and dry wind conditions. Copyright: © 2014 Wada et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v9_n10_p_Wada |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carbon 13 starch starch water Article biosynthesis carbohydrate synthesis cell metabolism controlled study degradation kinetics down regulation dry wind electrospray gene expression growth regulation mass spectrometer nonhuman photosynthesis physical chemistry plant osmotic adjustment plant water potential plant water relation polymerase chain reaction rice seed kernel temperature sensitivity wind anatomy and histology drug effects endosperm gene expression regulation genetics heat metabolism organ size Oryza osmosis time factor Endosperm Gene Expression Regulation, Plant Hot Temperature Organ Size Oryza Osmosis Photosynthesis Starch Time Factors Water Wind |
| spellingShingle |
carbon 13 starch starch water Article biosynthesis carbohydrate synthesis cell metabolism controlled study degradation kinetics down regulation dry wind electrospray gene expression growth regulation mass spectrometer nonhuman photosynthesis physical chemistry plant osmotic adjustment plant water potential plant water relation polymerase chain reaction rice seed kernel temperature sensitivity wind anatomy and histology drug effects endosperm gene expression regulation genetics heat metabolism organ size Oryza osmosis time factor Endosperm Gene Expression Regulation, Plant Hot Temperature Organ Size Oryza Osmosis Photosynthesis Starch Time Factors Water Wind Wada, H. Masumoto-Kubo, C. Gholipour, Y. Nonami, H. Tanaka, F. Erra-Balsells, R. Tsutsumi, K. Hiraoka, K. Morita, S. Rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under Foehn-induced dry wind |
| topic_facet |
carbon 13 starch starch water Article biosynthesis carbohydrate synthesis cell metabolism controlled study degradation kinetics down regulation dry wind electrospray gene expression growth regulation mass spectrometer nonhuman photosynthesis physical chemistry plant osmotic adjustment plant water potential plant water relation polymerase chain reaction rice seed kernel temperature sensitivity wind anatomy and histology drug effects endosperm gene expression regulation genetics heat metabolism organ size Oryza osmosis time factor Endosperm Gene Expression Regulation, Plant Hot Temperature Organ Size Oryza Osmosis Photosynthesis Starch Time Factors Water Wind |
| description |
Foehn-like extreme hot and dry wind conditions (34 °C, >2.5 kPa vapor pressure deficit, and 7 m s-1) strongly affect grain quality in rice (Oryza sativa L.). This is a current concern because of the increasing frequency and intensity of combined heat and water-deficit stress under climate change. Foehn-induced dry wind conditions during the grain-filling stage increase ring-shaped chalkiness as a result of spatiotemporal reduction in starch accumulation in the endosperm, but kernel growth is sometimes maintained by osmotic adjustment. Here, we assess the effects of dry wind on chalky ring formation in environmentally controlled growth chambers. Our results showed that hot and dry wind conditions that lasted for > 24 h dramatically increased chalky ring formation. Hot and dry wind conditions temporarily reduced panicle water potential to - 0.65 MPa; however, kernel growth was maintained by osmotic adjustment at control levels with increased transport of assimilate to the growing kernels. Dynamic tracer analysis with a nano-electrospray-ionization Orbitrap mass spectrometer and quantitative polymerase chain reaction analysis revealed that starch degradation was negligible in the short-term treatment. Overall expression of starch synthesis-related genes was found to be down-regulated at moderately low water potential. Because the events observed at low water potential preceded the packing of starch granules in cells, we concluded that reduced rates of starch biosynthesis play a central role in the events of cellular metabolism that are altered at osmotic adjustment, which leads to chalky ring formation under short-term hot and dry wind conditions. Copyright: © 2014 Wada et al. |
| format |
JOUR |
| author |
Wada, H. Masumoto-Kubo, C. Gholipour, Y. Nonami, H. Tanaka, F. Erra-Balsells, R. Tsutsumi, K. Hiraoka, K. Morita, S. |
| author_facet |
Wada, H. Masumoto-Kubo, C. Gholipour, Y. Nonami, H. Tanaka, F. Erra-Balsells, R. Tsutsumi, K. Hiraoka, K. Morita, S. |
| author_sort |
Wada, H. |
| title |
Rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under Foehn-induced dry wind |
| title_short |
Rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under Foehn-induced dry wind |
| title_full |
Rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under Foehn-induced dry wind |
| title_fullStr |
Rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under Foehn-induced dry wind |
| title_full_unstemmed |
Rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under Foehn-induced dry wind |
| title_sort |
rice chalky ring formation caused by temporal reduction in starch biosynthesis during osmotic adjustment under foehn-induced dry wind |
| url |
http://hdl.handle.net/20.500.12110/paper_19326203_v9_n10_p_Wada |
| work_keys_str_mv |
AT wadah ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind AT masumotokuboc ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind AT gholipoury ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind AT nonamih ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind AT tanakaf ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind AT errabalsellsr ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind AT tsutsumik ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind AT hiraokak ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind AT moritas ricechalkyringformationcausedbytemporalreductioninstarchbiosynthesisduringosmoticadjustmentunderfoehninduceddrywind |
| _version_ |
1807322006136291328 |