Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA

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The maintenance of genomic integrity is of main importance to the survival and health of organisms which are continuously exposed to genotoxic stress. Cells respond to DNA damage by activating survival pathways consisting of cell cycle checkpoints and repair mechanisms. However, the signal that trig...

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Autores principales:	Ogara, M.F., Sirkin, P.F., Carcagno, A.L., Marazita, M.C., Sonzogni, S.V., Ceruti, J.M., Cánepa, E.T.
Formato:	JOUR
Materias:	ATM protein ATR protein checkpoint kinase 1 checkpoint kinase 2 cyclin dependent kinase inhibitor 2D transcription factor E2F article cell cycle regulation cell function chromatin chromatin relaxation chromatin structure controlled study DNA repair enzyme activity genotoxicity human human cell protein induction signal transduction Cell Cycle Proteins Cell Line Chloroquine Chromatin Cyclin-Dependent Kinase Inhibitor p19 DNA Damage DNA Repair DNA-Binding Proteins E2F1 Transcription Factor Humans Models, Biological Mutagens Protein Kinases Protein-Serine-Threonine Kinases Signal Transduction Tumor Suppressor Proteins Ultraviolet Rays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v8_n4_p_Ogara
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_19326203_v8_n4_p_Ogara
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spelling	todo:paper_19326203_v8_n4_p_Ogara2023-10-03T16:35:33Z Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA Ogara, M.F. Sirkin, P.F. Carcagno, A.L. Marazita, M.C. Sonzogni, S.V. Ceruti, J.M. Cánepa, E.T. ATM protein ATR protein checkpoint kinase 1 checkpoint kinase 2 cyclin dependent kinase inhibitor 2D transcription factor E2F article cell cycle regulation cell function chromatin chromatin relaxation chromatin structure controlled study DNA repair enzyme activity genotoxicity human human cell protein induction signal transduction Cell Cycle Proteins Cell Line Chloroquine Chromatin Cyclin-Dependent Kinase Inhibitor p19 DNA Damage DNA Repair DNA-Binding Proteins E2F1 Transcription Factor Humans Models, Biological Mutagens Protein Kinases Protein-Serine-Threonine Kinases Signal Transduction Tumor Suppressor Proteins Ultraviolet Rays The maintenance of genomic integrity is of main importance to the survival and health of organisms which are continuously exposed to genotoxic stress. Cells respond to DNA damage by activating survival pathways consisting of cell cycle checkpoints and repair mechanisms. However, the signal that triggers the DNA damage response is not necessarily a direct detection of the primary DNA lesion. In fact, chromatin defects may serve as initiating signals to activate those mechanisms. If the modulation of chromatin structure could initiate a checkpoint response in a direct manner, this supposes the existence of specific chromatin sensors. p19INK4d, a member of the INK4 cell cycle inhibitors, plays a crucial role in regulating genomic stability and cell viability by enhancing DNA repair. Its expression is induced in cells injured by one of several genotoxic treatments like cis-platin, UV light or neocarzinostatin. Nevertheless, when exogenous DNA damaged molecules are introduced into the cell, this induction is not observed. Here, we show that p19INK4d is enhanced after chromatin relaxation even in the absence of DNA damage. This induction was shown to depend upon ATM/ATR, Chk1/Chk2 and E2F activity, as is the case of p19INK4d induction by endogenous DNA damage. Interestingly, p19INK4d improves DNA repair when the genotoxic damage is caused in a relaxed-chromatin context. These results suggest that changes in chromatin structure, and not DNA damage itself, is the actual trigger of p19INK4d induction. We propose that, in addition to its role as a cell cycle inhibitor, p19INK4d could participate in a signaling network directed to detecting and eventually responding to chromatin anomalies. © 2013 Ogara et al. Fil:Ogara, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carcagno, A.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marazita, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sonzogni, S.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceruti, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cánepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v8_n4_p_Ogara
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ATM protein ATR protein checkpoint kinase 1 checkpoint kinase 2 cyclin dependent kinase inhibitor 2D transcription factor E2F article cell cycle regulation cell function chromatin chromatin relaxation chromatin structure controlled study DNA repair enzyme activity genotoxicity human human cell protein induction signal transduction Cell Cycle Proteins Cell Line Chloroquine Chromatin Cyclin-Dependent Kinase Inhibitor p19 DNA Damage DNA Repair DNA-Binding Proteins E2F1 Transcription Factor Humans Models, Biological Mutagens Protein Kinases Protein-Serine-Threonine Kinases Signal Transduction Tumor Suppressor Proteins Ultraviolet Rays
spellingShingle	ATM protein ATR protein checkpoint kinase 1 checkpoint kinase 2 cyclin dependent kinase inhibitor 2D transcription factor E2F article cell cycle regulation cell function chromatin chromatin relaxation chromatin structure controlled study DNA repair enzyme activity genotoxicity human human cell protein induction signal transduction Cell Cycle Proteins Cell Line Chloroquine Chromatin Cyclin-Dependent Kinase Inhibitor p19 DNA Damage DNA Repair DNA-Binding Proteins E2F1 Transcription Factor Humans Models, Biological Mutagens Protein Kinases Protein-Serine-Threonine Kinases Signal Transduction Tumor Suppressor Proteins Ultraviolet Rays Ogara, M.F. Sirkin, P.F. Carcagno, A.L. Marazita, M.C. Sonzogni, S.V. Ceruti, J.M. Cánepa, E.T. Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
topic_facet	ATM protein ATR protein checkpoint kinase 1 checkpoint kinase 2 cyclin dependent kinase inhibitor 2D transcription factor E2F article cell cycle regulation cell function chromatin chromatin relaxation chromatin structure controlled study DNA repair enzyme activity genotoxicity human human cell protein induction signal transduction Cell Cycle Proteins Cell Line Chloroquine Chromatin Cyclin-Dependent Kinase Inhibitor p19 DNA Damage DNA Repair DNA-Binding Proteins E2F1 Transcription Factor Humans Models, Biological Mutagens Protein Kinases Protein-Serine-Threonine Kinases Signal Transduction Tumor Suppressor Proteins Ultraviolet Rays
description	The maintenance of genomic integrity is of main importance to the survival and health of organisms which are continuously exposed to genotoxic stress. Cells respond to DNA damage by activating survival pathways consisting of cell cycle checkpoints and repair mechanisms. However, the signal that triggers the DNA damage response is not necessarily a direct detection of the primary DNA lesion. In fact, chromatin defects may serve as initiating signals to activate those mechanisms. If the modulation of chromatin structure could initiate a checkpoint response in a direct manner, this supposes the existence of specific chromatin sensors. p19INK4d, a member of the INK4 cell cycle inhibitors, plays a crucial role in regulating genomic stability and cell viability by enhancing DNA repair. Its expression is induced in cells injured by one of several genotoxic treatments like cis-platin, UV light or neocarzinostatin. Nevertheless, when exogenous DNA damaged molecules are introduced into the cell, this induction is not observed. Here, we show that p19INK4d is enhanced after chromatin relaxation even in the absence of DNA damage. This induction was shown to depend upon ATM/ATR, Chk1/Chk2 and E2F activity, as is the case of p19INK4d induction by endogenous DNA damage. Interestingly, p19INK4d improves DNA repair when the genotoxic damage is caused in a relaxed-chromatin context. These results suggest that changes in chromatin structure, and not DNA damage itself, is the actual trigger of p19INK4d induction. We propose that, in addition to its role as a cell cycle inhibitor, p19INK4d could participate in a signaling network directed to detecting and eventually responding to chromatin anomalies. © 2013 Ogara et al.
format	JOUR
author	Ogara, M.F. Sirkin, P.F. Carcagno, A.L. Marazita, M.C. Sonzogni, S.V. Ceruti, J.M. Cánepa, E.T.
author_facet	Ogara, M.F. Sirkin, P.F. Carcagno, A.L. Marazita, M.C. Sonzogni, S.V. Ceruti, J.M. Cánepa, E.T.
author_sort	Ogara, M.F.
title	Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_short	Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_full	Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_fullStr	Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_full_unstemmed	Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA
title_sort	chromatin relaxation-mediated induction of p19ink4d increases the ability of cells to repair damaged dna
url	http://hdl.handle.net/20.500.12110/paper_19326203_v8_n4_p_Ogara
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_version_	1782027921771200512

Chromatin Relaxation-Mediated Induction of p19INK4d Increases the Ability of Cells to Repair Damaged DNA

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