EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation

Background: Retinotopic projection onto the tectum/colliculus constitutes the most studied model of topographic mapping and Eph receptors and their ligands, the ephrins, are the best characterized molecular system involved in this process. Ephrin-As, expressed in an increasing rostro-caudal gradient...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ortalli, A.L., Fiore, L., Di Napoli, J., Rapacioli, M., Salierno, M., Etchenique, R., Flores, V., Sanchez, V., Carri, N.G., Scicolone, G.
Formato:	JOUR
Materias:	ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v7_n6_p_Ortalli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_19326203_v7_n6_p_Ortalli
record_format	dspace
spelling	todo:paper_19326203_v7_n6_p_Ortalli2023-10-03T16:35:22Z EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation Ortalli, A.L. Fiore, L. Di Napoli, J. Rapacioli, M. Salierno, M. Etchenique, R. Flores, V. Sanchez, V. Carri, N.G. Scicolone, G. ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways Background: Retinotopic projection onto the tectum/colliculus constitutes the most studied model of topographic mapping and Eph receptors and their ligands, the ephrins, are the best characterized molecular system involved in this process. Ephrin-As, expressed in an increasing rostro-caudal gradient in the tectum/colliculus, repel temporal retinal ganglion cell (RGC) axons from the caudal tectum and inhibit their branching posterior to their termination zones. However, there are conflicting data regarding the nature of the second force that guides nasal axons to invade and branch only in the caudal tectum/colliculus. The predominant model postulates that this second force is produced by a decreasing rostro-caudal gradient of EphA7 which repels nasal optic fibers and prevents their branching in the rostral tectum/colliculus. However, as optic fibers invade the tectum/colliculus growing throughout this gradient, this model cannot explain how the axons grow throughout this repellent molecule. Methodology/Principal Findings: By using chicken retinal cultures we showed that EphA3 ectodomain stimulates nasal RGC axon growth in a concentration dependent way. Moreover, we showed that nasal axons choose growing on EphA3-expressing cells and that EphA3 diminishes the density of interstitial filopodia in nasal RGC axons. Accordingly, in vivo EphA3 ectodomain misexpression directs nasal optic fibers toward the caudal tectum preventing their branching in the rostral tectum. Conclusions: We demonstrated in vitro and in vivo that EphA3 ectodomain (which is expressed in a decreasing rostro-caudal gradient in the tectum) is necessary for topographic mapping by stimulating the nasal axon growth toward the caudal tectum and inhibiting their branching in the rostral tectum. Furthermore, the ability of EphA3 of stimulating axon growth allows understanding how optic fibers invade the tectum growing throughout this molecular gradient. Therefore, opposing tectal gradients of repellent ephrin-As and of axon growth stimulating EphA3 complement each other to map optic fibers along the rostro-caudal tectal axis. © 2012 Ortalli et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v7_n6_p_Ortalli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways
spellingShingle	ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways Ortalli, A.L. Fiore, L. Di Napoli, J. Rapacioli, M. Salierno, M. Etchenique, R. Flores, V. Sanchez, V. Carri, N.G. Scicolone, G. EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation
topic_facet	ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways
description	Background: Retinotopic projection onto the tectum/colliculus constitutes the most studied model of topographic mapping and Eph receptors and their ligands, the ephrins, are the best characterized molecular system involved in this process. Ephrin-As, expressed in an increasing rostro-caudal gradient in the tectum/colliculus, repel temporal retinal ganglion cell (RGC) axons from the caudal tectum and inhibit their branching posterior to their termination zones. However, there are conflicting data regarding the nature of the second force that guides nasal axons to invade and branch only in the caudal tectum/colliculus. The predominant model postulates that this second force is produced by a decreasing rostro-caudal gradient of EphA7 which repels nasal optic fibers and prevents their branching in the rostral tectum/colliculus. However, as optic fibers invade the tectum/colliculus growing throughout this gradient, this model cannot explain how the axons grow throughout this repellent molecule. Methodology/Principal Findings: By using chicken retinal cultures we showed that EphA3 ectodomain stimulates nasal RGC axon growth in a concentration dependent way. Moreover, we showed that nasal axons choose growing on EphA3-expressing cells and that EphA3 diminishes the density of interstitial filopodia in nasal RGC axons. Accordingly, in vivo EphA3 ectodomain misexpression directs nasal optic fibers toward the caudal tectum preventing their branching in the rostral tectum. Conclusions: We demonstrated in vitro and in vivo that EphA3 ectodomain (which is expressed in a decreasing rostro-caudal gradient in the tectum) is necessary for topographic mapping by stimulating the nasal axon growth toward the caudal tectum and inhibiting their branching in the rostral tectum. Furthermore, the ability of EphA3 of stimulating axon growth allows understanding how optic fibers invade the tectum growing throughout this molecular gradient. Therefore, opposing tectal gradients of repellent ephrin-As and of axon growth stimulating EphA3 complement each other to map optic fibers along the rostro-caudal tectal axis. © 2012 Ortalli et al.
format	JOUR
author	Ortalli, A.L. Fiore, L. Di Napoli, J. Rapacioli, M. Salierno, M. Etchenique, R. Flores, V. Sanchez, V. Carri, N.G. Scicolone, G.
author_facet	Ortalli, A.L. Fiore, L. Di Napoli, J. Rapacioli, M. Salierno, M. Etchenique, R. Flores, V. Sanchez, V. Carri, N.G. Scicolone, G.
author_sort	Ortalli, A.L.
title	EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation
title_short	EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation
title_full	EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation
title_fullStr	EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation
title_full_unstemmed	EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation
title_sort	epha3 expressed in the chicken tectum stimulates nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation
url	http://hdl.handle.net/20.500.12110/paper_19326203_v7_n6_p_Ortalli
work_keys_str_mv	AT ortallial epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT fiorel epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT dinapolij epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT rapaciolim epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT saliernom epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT etcheniquer epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT floresv epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT sanchezv epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT carring epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation AT scicoloneg epha3expressedinthechickentectumstimulatesnasalretinalganglioncellaxongrowthandisrequiredforretinotectaltopographicmapformation
_version_	1782025940782546944

EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation

Ejemplares similares