Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes?

Cytological diploidization process is different in autopolyploid and allopolyploid species. Colchicine applied at the onset of meiosis suppresses the effect of pairing regulator genes resulting multivalents formation in bivalent-forming species. Colchicine treated maizes (4x = 2n = 20, AmAmBmBm) sho...

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Autores principales:	Poggio, L., González, G.E.
Formato:	JOUR
Materias:	colchicine Article chromosome bivalent chromosome pairing cytology diploidy gene expression gene locus genetic variability genus hexaploidy maize nonhuman octoploidy paleopolyploid plant genome polyploidy regulator gene structural homology Zea Zea perennis genetics meiosis plant chromosome Chromosomes, Plant Colchicine Diploidy Genes, Regulator Meiosis Zea mays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v13_n1_p_Poggio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_19326203_v13_n1_p_Poggio2023-10-03T16:34:55Z Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes? Poggio, L. González, G.E. colchicine colchicine Article chromosome bivalent chromosome pairing cytology diploidy gene expression gene locus genetic variability genus hexaploidy maize nonhuman octoploidy paleopolyploid plant genome polyploidy regulator gene structural homology Zea Zea perennis diploidy genetics meiosis plant chromosome Chromosomes, Plant Colchicine Diploidy Genes, Regulator Meiosis Zea mays Cytological diploidization process is different in autopolyploid and allopolyploid species. Colchicine applied at the onset of meiosis suppresses the effect of pairing regulator genes resulting multivalents formation in bivalent-forming species. Colchicine treated maizes (4x = 2n = 20, AmAmBmBm) showed up to 5IV, suggesting pairing between chromosomes from genomes homoeologous Am and Bm. In untreated individuals of the alloautooctoploid Zea perennis (8x = 2n = 40, ApApAp´Ap´Bp1Bp1Bp2Bp2) the most frequent configuration was 5IV +10II (formed by A and B genomes, respectively). The colchicine treated Z. perennis show up to 10IV revealing higher affinity within genomes A and B, but any homology among them. These results suggest the presence of a paring regulator locus (PrZ) in maize and Z. perennis, whose expression is suppressed by colchicine. It could be postulated that in Z. perennis, PrZ would affect independently the genomes A and B, being relevant the threshold of homology, the fidelity of pairing in each genomes and the ploidy level. Cytological analysis of the treated hexaploid hybrids (6x = 2n = 30), with Z. perennis as a parental, strongly suggests that PrZ is less effective in only one doses. This conclusion was reinforced by the homoeologous pairing observed in untreated dihaploid maizes, which showed up to 5II. Meiotic behaviour of individuals treated with different doses of colchicine allowed to postulate that PrZ affect the homoeologous association by controlling entire genomes (Am or Bm) rather than individual chromosomes. Based on cytological and statistical results it is possible to propose that the cytological diploidization in Zea species occurs by restriction of pairing between homoeologous chromosomes or by genetical divergence of the homoeologous chromosomes, as was observed in untreated Z. mays ssp. parviglumis. These are independent but complementary systems and could be acting jointly in the same nucleus. © 2018 Poggio, González. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v13_n1_p_Poggio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	colchicine colchicine Article chromosome bivalent chromosome pairing cytology diploidy gene expression gene locus genetic variability genus hexaploidy maize nonhuman octoploidy paleopolyploid plant genome polyploidy regulator gene structural homology Zea Zea perennis diploidy genetics meiosis plant chromosome Chromosomes, Plant Colchicine Diploidy Genes, Regulator Meiosis Zea mays
spellingShingle	colchicine colchicine Article chromosome bivalent chromosome pairing cytology diploidy gene expression gene locus genetic variability genus hexaploidy maize nonhuman octoploidy paleopolyploid plant genome polyploidy regulator gene structural homology Zea Zea perennis diploidy genetics meiosis plant chromosome Chromosomes, Plant Colchicine Diploidy Genes, Regulator Meiosis Zea mays Poggio, L. González, G.E. Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes?
topic_facet	colchicine colchicine Article chromosome bivalent chromosome pairing cytology diploidy gene expression gene locus genetic variability genus hexaploidy maize nonhuman octoploidy paleopolyploid plant genome polyploidy regulator gene structural homology Zea Zea perennis diploidy genetics meiosis plant chromosome Chromosomes, Plant Colchicine Diploidy Genes, Regulator Meiosis Zea mays
description	Cytological diploidization process is different in autopolyploid and allopolyploid species. Colchicine applied at the onset of meiosis suppresses the effect of pairing regulator genes resulting multivalents formation in bivalent-forming species. Colchicine treated maizes (4x = 2n = 20, AmAmBmBm) showed up to 5IV, suggesting pairing between chromosomes from genomes homoeologous Am and Bm. In untreated individuals of the alloautooctoploid Zea perennis (8x = 2n = 40, ApApAp´Ap´Bp1Bp1Bp2Bp2) the most frequent configuration was 5IV +10II (formed by A and B genomes, respectively). The colchicine treated Z. perennis show up to 10IV revealing higher affinity within genomes A and B, but any homology among them. These results suggest the presence of a paring regulator locus (PrZ) in maize and Z. perennis, whose expression is suppressed by colchicine. It could be postulated that in Z. perennis, PrZ would affect independently the genomes A and B, being relevant the threshold of homology, the fidelity of pairing in each genomes and the ploidy level. Cytological analysis of the treated hexaploid hybrids (6x = 2n = 30), with Z. perennis as a parental, strongly suggests that PrZ is less effective in only one doses. This conclusion was reinforced by the homoeologous pairing observed in untreated dihaploid maizes, which showed up to 5II. Meiotic behaviour of individuals treated with different doses of colchicine allowed to postulate that PrZ affect the homoeologous association by controlling entire genomes (Am or Bm) rather than individual chromosomes. Based on cytological and statistical results it is possible to propose that the cytological diploidization in Zea species occurs by restriction of pairing between homoeologous chromosomes or by genetical divergence of the homoeologous chromosomes, as was observed in untreated Z. mays ssp. parviglumis. These are independent but complementary systems and could be acting jointly in the same nucleus. © 2018 Poggio, González. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
format	JOUR
author	Poggio, L. González, G.E.
author_facet	Poggio, L. González, G.E.
author_sort	Poggio, L.
title	Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes?
title_short	Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes?
title_full	Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes?
title_fullStr	Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes?
title_full_unstemmed	Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes?
title_sort	cytological diploidization of paleopolyploid genus zea: divergence between homoeologous chromosomes or activity of pairing regulator genes?
url	http://hdl.handle.net/20.500.12110/paper_19326203_v13_n1_p_Poggio
work_keys_str_mv	AT poggiol cytologicaldiploidizationofpaleopolyploidgenuszeadivergencebetweenhomoeologouschromosomesoractivityofpairingregulatorgenes AT gonzalezge cytologicaldiploidizationofpaleopolyploidgenuszeadivergencebetweenhomoeologouschromosomesoractivityofpairingregulatorgenes
_version_	1782030442277371904

Cytological diploidization of paleopolyploid genus Zea: Divergence between homoeologous chromosomes or activity of pairing regulator genes?

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