Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4

Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiologic...

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Autores principales:	Sacco, N.J., Bonetto, M.C., Cortón, E.
Formato:	JOUR
Materias:	canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v12_n2_p_Sacco
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_19326203_v12_n2_p_Sacco2023-10-03T16:34:47Z Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4 Sacco, N.J. Bonetto, M.C. Cortón, E. canthaxanthin RNA 16S canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Actinobacteria Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiological and morphological characteristics, as well as by its 16S rRNA sequence analysis. Furthermore, the current density production and electron transfer mechanisms were investigated using bioelectrochemical methods. The chronoamperometric data showed that the biofilm of Dietzia sp. RNV-4 grew as the current increased with time, reaching a maximum of 176.6 ± 66.1 mA/m2 at the end of the experiment (7 d); this highly suggests that the current was generated by the biofilm. The main electron transfer mechanism, indicated by the cyclic voltammograms, was due to secreted redox mediators. By high performance liquid chromatography, canthaxanthin was identified as the main compound involved in charge transfer between the bacteria and the solid electrodes. Dietzia sp. RNV-4 was used as biological material in a microbial fuel cell (MFC) and the current density production was 299.4 ± 40.2 mA/m2. This is the first time that Dietzia sp. RNV-4 has been electrochemically characterized and identified as a new electrogenic strain. © 2017 Sacco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Sacco, N.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bonetto, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v12_n2_p_Sacco
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	canthaxanthin RNA 16S canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Actinobacteria Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S
spellingShingle	canthaxanthin RNA 16S canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Actinobacteria Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S Sacco, N.J. Bonetto, M.C. Cortón, E. Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
topic_facet	canthaxanthin RNA 16S canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Actinobacteria Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S
description	Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiological and morphological characteristics, as well as by its 16S rRNA sequence analysis. Furthermore, the current density production and electron transfer mechanisms were investigated using bioelectrochemical methods. The chronoamperometric data showed that the biofilm of Dietzia sp. RNV-4 grew as the current increased with time, reaching a maximum of 176.6 ± 66.1 mA/m2 at the end of the experiment (7 d); this highly suggests that the current was generated by the biofilm. The main electron transfer mechanism, indicated by the cyclic voltammograms, was due to secreted redox mediators. By high performance liquid chromatography, canthaxanthin was identified as the main compound involved in charge transfer between the bacteria and the solid electrodes. Dietzia sp. RNV-4 was used as biological material in a microbial fuel cell (MFC) and the current density production was 299.4 ± 40.2 mA/m2. This is the first time that Dietzia sp. RNV-4 has been electrochemically characterized and identified as a new electrogenic strain. © 2017 Sacco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
format	JOUR
author	Sacco, N.J. Bonetto, M.C. Cortón, E.
author_facet	Sacco, N.J. Bonetto, M.C. Cortón, E.
author_sort	Sacco, N.J.
title	Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_short	Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_full	Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_fullStr	Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_full_unstemmed	Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_sort	isolation and characterization of a novel electrogenic bacterium, dietzia sp. rnv-4
url	http://hdl.handle.net/20.500.12110/paper_19326203_v12_n2_p_Sacco
work_keys_str_mv	AT sacconj isolationandcharacterizationofanovelelectrogenicbacteriumdietziasprnv4 AT bonettomc isolationandcharacterizationofanovelelectrogenicbacteriumdietziasprnv4 AT cortone isolationandcharacterizationofanovelelectrogenicbacteriumdietziasprnv4
_version_	1782030104556208128

Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4

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