Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences

A molecular phylogenetic analysis of Fusarium poae isolates from South America (Argentina) and Europe (mainly England, Germany, Italy) was performed using 98 F. poae, four Fusarium culmorum, two Fusarium sporotrichioides and one Fusarium langsethiae isolates. Phylogenetic analyses were performed usi...

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Autores principales:	Stenglein, S.A., Rodriguero, M.S., Chandler, E., Jennings, P., Salerno, G.L., Nicholson, P.
Formato:	JOUR
Materias:	EF1-α Fusarium poae MtSSU rDNA Tri5 elongation factor 1 fungal DNA lyase mitochondrial DNA ribosome DNA trichodiene synthetase fungus genetic analysis isolated population molecular analysis phylogenetics range size taxonomy Argentina article barley classification DNA sequence Europe Fusarium genetics intron isolation and purification microbiology phylogeny polymerase chain reaction species difference wheat Carbon-Carbon Lyases DNA, Fungal DNA, Mitochondrial DNA, Ribosomal Hordeum Introns Peptide Elongation Factor 1 Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Species Specificity Triticum South America Fusarium culmorum Fusarium langsethiae Fusarium sporotrichioides
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_18786146_v114_n1_p96_Stenglein
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_18786146_v114_n1_p96_Stenglein2023-10-03T16:34:20Z Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences Stenglein, S.A. Rodriguero, M.S. Chandler, E. Jennings, P. Salerno, G.L. Nicholson, P. EF1-α Fusarium poae MtSSU rDNA Tri5 elongation factor 1 fungal DNA lyase mitochondrial DNA ribosome DNA trichodiene synthetase fungus genetic analysis isolated population molecular analysis phylogenetics range size taxonomy Argentina article barley classification DNA sequence Europe Fusarium genetics intron isolation and purification microbiology phylogeny polymerase chain reaction species difference wheat Argentina Carbon-Carbon Lyases DNA, Fungal DNA, Mitochondrial DNA, Ribosomal Europe Fusarium Hordeum Introns Peptide Elongation Factor 1 Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Species Specificity Triticum Europe South America Fusarium Fusarium culmorum Fusarium langsethiae Fusarium poae Fusarium sporotrichioides A molecular phylogenetic analysis of Fusarium poae isolates from South America (Argentina) and Europe (mainly England, Germany, Italy) was performed using 98 F. poae, four Fusarium culmorum, two Fusarium sporotrichioides and one Fusarium langsethiae isolates. Phylogenetic analyses were performed using nuclear (translation elongation factor 1-alpha, EF-1α) and mitochondrial (mitochondrial small subunit rDNA, mtSSU) sequences. Partitioned (each dataset separately) and combined (EF-1α+. mtSSU) analyses did not reveal any clear correlations from the inferred branching topology, between the distribution of observed haplotypes and the geographic origin and/or host species. Results from the present study confirmed that isolates from F. poae form a monophyletic group, and the low variability within isolates from a broad geographic range suggests a common lineage history. Among F. poae isolates from Argentina, however, some were found to possess an insert within mtSSU with structural similarities to group IC2 introns. F. poae isolates differing by the presence/absence of a mtSSU insertion were characterized further by analysis of a portion of the Tri5 gene, but this sequence was unable to reveal variability. The presence of this insert only within isolates from Argentina suggests that evolutionary events (insertions/deletions) are probably taking place within the Argentinian F. poae isolates, and that the acquisition of this insert occurred after geographic isolation of the Argentinian and European populations. © 2009 The British Mycological Society. Fil:Rodriguero, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salerno, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_18786146_v114_n1_p96_Stenglein
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	EF1-α Fusarium poae MtSSU rDNA Tri5 elongation factor 1 fungal DNA lyase mitochondrial DNA ribosome DNA trichodiene synthetase fungus genetic analysis isolated population molecular analysis phylogenetics range size taxonomy Argentina article barley classification DNA sequence Europe Fusarium genetics intron isolation and purification microbiology phylogeny polymerase chain reaction species difference wheat Argentina Carbon-Carbon Lyases DNA, Fungal DNA, Mitochondrial DNA, Ribosomal Europe Fusarium Hordeum Introns Peptide Elongation Factor 1 Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Species Specificity Triticum Europe South America Fusarium Fusarium culmorum Fusarium langsethiae Fusarium poae Fusarium sporotrichioides
spellingShingle	EF1-α Fusarium poae MtSSU rDNA Tri5 elongation factor 1 fungal DNA lyase mitochondrial DNA ribosome DNA trichodiene synthetase fungus genetic analysis isolated population molecular analysis phylogenetics range size taxonomy Argentina article barley classification DNA sequence Europe Fusarium genetics intron isolation and purification microbiology phylogeny polymerase chain reaction species difference wheat Argentina Carbon-Carbon Lyases DNA, Fungal DNA, Mitochondrial DNA, Ribosomal Europe Fusarium Hordeum Introns Peptide Elongation Factor 1 Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Species Specificity Triticum Europe South America Fusarium Fusarium culmorum Fusarium langsethiae Fusarium poae Fusarium sporotrichioides Stenglein, S.A. Rodriguero, M.S. Chandler, E. Jennings, P. Salerno, G.L. Nicholson, P. Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences
topic_facet	EF1-α Fusarium poae MtSSU rDNA Tri5 elongation factor 1 fungal DNA lyase mitochondrial DNA ribosome DNA trichodiene synthetase fungus genetic analysis isolated population molecular analysis phylogenetics range size taxonomy Argentina article barley classification DNA sequence Europe Fusarium genetics intron isolation and purification microbiology phylogeny polymerase chain reaction species difference wheat Argentina Carbon-Carbon Lyases DNA, Fungal DNA, Mitochondrial DNA, Ribosomal Europe Fusarium Hordeum Introns Peptide Elongation Factor 1 Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Species Specificity Triticum Europe South America Fusarium Fusarium culmorum Fusarium langsethiae Fusarium poae Fusarium sporotrichioides
description	A molecular phylogenetic analysis of Fusarium poae isolates from South America (Argentina) and Europe (mainly England, Germany, Italy) was performed using 98 F. poae, four Fusarium culmorum, two Fusarium sporotrichioides and one Fusarium langsethiae isolates. Phylogenetic analyses were performed using nuclear (translation elongation factor 1-alpha, EF-1α) and mitochondrial (mitochondrial small subunit rDNA, mtSSU) sequences. Partitioned (each dataset separately) and combined (EF-1α+. mtSSU) analyses did not reveal any clear correlations from the inferred branching topology, between the distribution of observed haplotypes and the geographic origin and/or host species. Results from the present study confirmed that isolates from F. poae form a monophyletic group, and the low variability within isolates from a broad geographic range suggests a common lineage history. Among F. poae isolates from Argentina, however, some were found to possess an insert within mtSSU with structural similarities to group IC2 introns. F. poae isolates differing by the presence/absence of a mtSSU insertion were characterized further by analysis of a portion of the Tri5 gene, but this sequence was unable to reveal variability. The presence of this insert only within isolates from Argentina suggests that evolutionary events (insertions/deletions) are probably taking place within the Argentinian F. poae isolates, and that the acquisition of this insert occurred after geographic isolation of the Argentinian and European populations. © 2009 The British Mycological Society.
format	JOUR
author	Stenglein, S.A. Rodriguero, M.S. Chandler, E. Jennings, P. Salerno, G.L. Nicholson, P.
author_facet	Stenglein, S.A. Rodriguero, M.S. Chandler, E. Jennings, P. Salerno, G.L. Nicholson, P.
author_sort	Stenglein, S.A.
title	Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences
title_short	Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences
title_full	Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences
title_fullStr	Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences
title_full_unstemmed	Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences
title_sort	phylogenetic relationships of fusarium poae based on ef-1α and mtssu sequences
url	http://hdl.handle.net/20.500.12110/paper_18786146_v114_n1_p96_Stenglein
work_keys_str_mv	AT stengleinsa phylogeneticrelationshipsoffusariumpoaebasedonef1aandmtssusequences AT rodrigueroms phylogeneticrelationshipsoffusariumpoaebasedonef1aandmtssusequences AT chandlere phylogeneticrelationshipsoffusariumpoaebasedonef1aandmtssusequences AT jenningsp phylogeneticrelationshipsoffusariumpoaebasedonef1aandmtssusequences AT salernogl phylogeneticrelationshipsoffusariumpoaebasedonef1aandmtssusequences AT nicholsonp phylogeneticrelationshipsoffusariumpoaebasedonef1aandmtssusequences
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Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences

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