Disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium

The polyphenol epigallocatechin-3-gallate (EGCG), is the main active catechin of the green tea. It exerts a wide range of biochemical and pharmacological effects on different animal cells. EGCG acts as a scavenger of growth factors and it was demonstrated its antiproliferative and proapoptotic role...

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Autores principales: Jungblut, L.D., Paz, D.A., Pozzi, A.G.
Formato: JOUR
Materias:
Growth factors
Olfactory neurons
Tadpoles
epigallocatechin gallate
mitogen activated protein kinase
mitogen activated protein kinase 1
mitogen activated protein kinase 3
phosphatidylinositol 3 kinase
animal cell
animal tissue
apoptosis
article
cell proliferation
controlled study
nervous system development
nonhuman
olfactory epithelium
priority journal
protein phosphorylation
signal transduction
tadpole
Amphibia
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_18750443_v3_n_p20_Jungblut
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_18750443_v3_n_p20_Jungblut
record_format dspace
spelling todo:paper_18750443_v3_n_p20_Jungblut2023-10-03T16:34:10Z Disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium Jungblut, L.D. Paz, D.A. Pozzi, A.G. Growth factors Olfactory neurons Tadpoles epigallocatechin gallate mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 phosphatidylinositol 3 kinase animal cell animal tissue apoptosis article cell proliferation controlled study nervous system development nonhuman olfactory epithelium priority journal protein phosphorylation signal transduction tadpole Amphibia Animalia The polyphenol epigallocatechin-3-gallate (EGCG), is the main active catechin of the green tea. It exerts a wide range of biochemical and pharmacological effects on different animal cells. EGCG acts as a scavenger of growth factors and it was demonstrated its antiproliferative and proapoptotic role in different cancer cells. Nevertheless, little is known about the effect of EGCG on normal cells. In the present work, we have studied the EGCG effect on the proliferation/apoptosis balance in the olfactory epithelium of amphibian tadpoles. Animals treated with EGCG showed an increase in the number of apoptotic cells and a decrease in the basal proliferation. In addition, EGCG showed differential effect on the two classical growth factors signalling pathways, MAPK and PI3K route. EGCG treatment triggered a drop in phospho-ERK1/2 level without affecting the phospho-AKT level. These findings demonstrate the antiproliferative and proapoptotic effect of EGCG on normal olfactory cells and postulate the olfactory epithelium as a good experimental model for the study of the factors involved in neurogenesis process. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_18750443_v3_n_p20_Jungblut
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Growth factors
Olfactory neurons
Tadpoles
epigallocatechin gallate
mitogen activated protein kinase
mitogen activated protein kinase 1
mitogen activated protein kinase 3
phosphatidylinositol 3 kinase
animal cell
animal tissue
apoptosis
article
cell proliferation
controlled study
nervous system development
nonhuman
olfactory epithelium
priority journal
protein phosphorylation
signal transduction
tadpole
Amphibia
Animalia
spellingShingle Growth factors
Olfactory neurons
Tadpoles
epigallocatechin gallate
mitogen activated protein kinase
mitogen activated protein kinase 1
mitogen activated protein kinase 3
phosphatidylinositol 3 kinase
animal cell
animal tissue
apoptosis
article
cell proliferation
controlled study
nervous system development
nonhuman
olfactory epithelium
priority journal
protein phosphorylation
signal transduction
tadpole
Amphibia
Animalia
Jungblut, L.D.
Paz, D.A.
Pozzi, A.G.
Disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium
topic_facet Growth factors
Olfactory neurons
Tadpoles
epigallocatechin gallate
mitogen activated protein kinase
mitogen activated protein kinase 1
mitogen activated protein kinase 3
phosphatidylinositol 3 kinase
animal cell
animal tissue
apoptosis
article
cell proliferation
controlled study
nervous system development
nonhuman
olfactory epithelium
priority journal
protein phosphorylation
signal transduction
tadpole
Amphibia
Animalia
description The polyphenol epigallocatechin-3-gallate (EGCG), is the main active catechin of the green tea. It exerts a wide range of biochemical and pharmacological effects on different animal cells. EGCG acts as a scavenger of growth factors and it was demonstrated its antiproliferative and proapoptotic role in different cancer cells. Nevertheless, little is known about the effect of EGCG on normal cells. In the present work, we have studied the EGCG effect on the proliferation/apoptosis balance in the olfactory epithelium of amphibian tadpoles. Animals treated with EGCG showed an increase in the number of apoptotic cells and a decrease in the basal proliferation. In addition, EGCG showed differential effect on the two classical growth factors signalling pathways, MAPK and PI3K route. EGCG treatment triggered a drop in phospho-ERK1/2 level without affecting the phospho-AKT level. These findings demonstrate the antiproliferative and proapoptotic effect of EGCG on normal olfactory cells and postulate the olfactory epithelium as a good experimental model for the study of the factors involved in neurogenesis process.
format JOUR
author Jungblut, L.D.
Paz, D.A.
Pozzi, A.G.
author_facet Jungblut, L.D.
Paz, D.A.
Pozzi, A.G.
author_sort Jungblut, L.D.
title Disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium
title_short Disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium
title_full Disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium
title_fullStr Disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium
title_full_unstemmed Disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium
title_sort disruptive effect of epigallocatechin-3-gallate on the proliferation/ apoptosis balance in the olfactory epithelium
url http://hdl.handle.net/20.500.12110/paper_18750443_v3_n_p20_Jungblut
work_keys_str_mv AT jungblutld disruptiveeffectofepigallocatechin3gallateontheproliferationapoptosisbalanceintheolfactoryepithelium
AT pazda disruptiveeffectofepigallocatechin3gallateontheproliferationapoptosisbalanceintheolfactoryepithelium
AT pozziag disruptiveeffectofepigallocatechin3gallateontheproliferationapoptosisbalanceintheolfactoryepithelium
_version_ 1807318561042989056

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