Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell...

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Detalles Bibliográficos
Autores principales: Questa, M., Romorini, L., Blüguermann, C., Solari, C.M., Neiman, G., Luzzani, C., Scassa, M.T., Sevlever, G.E., Guberman, A.S., Miriuka, S.G.
Formato: JOUR
Materias:
kruppel like factor 4
Myc protein
octamer transcription factor 4
transcription factor Sox2
POU5F1 protein, human
transcription factor
Article
cell differentiation
controlled study
CpG island
fibroblast
gene expression
human
human cell
human embryonic stem cell
hypoxic cell
in vitro study
in vivo study
nuclear reprogramming
pluripotent stem cell
prepuce
priority journal
stem cell line
cell culture
comparative genomic hybridization
cytology
DNA methylation
fluorescence microscopy
genetics
induced pluripotent stem cell
karyotype
male
metabolism
promoter region
real time polymerase chain reaction
Cell Differentiation
Cells, Cultured
Cellular Reprogramming
Comparative Genomic Hybridization
DNA Methylation
Fibroblasts
Foreskin
Humans
Induced Pluripotent Stem Cells
Karyotype
Male
Microscopy, Fluorescence
Octamer Transcription Factor-3
Promoter Regions, Genetic
Real-Time Polymerase Chain Reaction
Transcription Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_18735061_v16_n2_p300_Questa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls. © 2015 The Authors.

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