Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell...

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Autores principales:	Questa, M., Romorini, L., Blüguermann, C., Solari, C.M., Neiman, G., Luzzani, C., Scassa, M.T., Sevlever, G.E., Guberman, A.S., Miriuka, S.G.
Formato:	JOUR
Materias:	kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_18735061_v16_n2_p300_Questa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_18735061_v16_n2_p300_Questa
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spelling	todo:paper_18735061_v16_n2_p300_Questa2023-10-03T16:34:01Z Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts Questa, M. Romorini, L. Blüguermann, C. Solari, C.M. Neiman, G. Luzzani, C. Scassa, M.T. Sevlever, G.E. Guberman, A.S. Miriuka, S.G. kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 octamer transcription factor 4 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fibroblast fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism prepuce promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls. © 2015 The Authors. Fil:Questa, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romorini, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scassa, M.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_18735061_v16_n2_p300_Questa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 octamer transcription factor 4 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fibroblast fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism prepuce promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors
spellingShingle	kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 octamer transcription factor 4 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fibroblast fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism prepuce promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors Questa, M. Romorini, L. Blüguermann, C. Solari, C.M. Neiman, G. Luzzani, C. Scassa, M.T. Sevlever, G.E. Guberman, A.S. Miriuka, S.G. Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
topic_facet	kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 octamer transcription factor 4 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fibroblast fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism prepuce promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors
description	Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls. © 2015 The Authors.
format	JOUR
author	Questa, M. Romorini, L. Blüguermann, C. Solari, C.M. Neiman, G. Luzzani, C. Scassa, M.T. Sevlever, G.E. Guberman, A.S. Miriuka, S.G.
author_facet	Questa, M. Romorini, L. Blüguermann, C. Solari, C.M. Neiman, G. Luzzani, C. Scassa, M.T. Sevlever, G.E. Guberman, A.S. Miriuka, S.G.
author_sort	Questa, M.
title	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_short	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_full	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_fullStr	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_full_unstemmed	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_sort	generation of ipsc line ipsc-fh2.1 in hypoxic conditions from human foreskin fibroblasts
url	http://hdl.handle.net/20.500.12110/paper_18735061_v16_n2_p300_Questa
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Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

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