3D microscopy by orbital radially modulated scan

Mostrar todas las versiones(3)

In this work, we present an alternative imaging method based on the orbital scanning of a laser excitation beam around the object of interest. This technology is capable of producing tridimensional images of fluorescent structures with nanometrical resolution in a few milliseconds. The method relies...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Zaza, C., Gabriel, M., Estrada, L.C.
Formato: JOUR
Materias:
Fluorescence
Microscopy
Single particle tracking
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_18501168_v29_n1_p12_Zaza
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_18501168_v29_n1_p12_Zaza
record_format dspace
spelling todo:paper_18501168_v29_n1_p12_Zaza2023-10-03T16:33:14Z 3D microscopy by orbital radially modulated scan Zaza, C. Gabriel, M. Estrada, L.C. Fluorescence Microscopy Single particle tracking In this work, we present an alternative imaging method based on the orbital scanning of a laser excitation beam around the object of interest. This technology is capable of producing tridimensional images of fluorescent structures with nanometrical resolution in a few milliseconds. The method relies on the fact that when the excitation beam is near a fluorescent object, the emitted light from the object depends on the distance between its surface and the excitation beam. By modulating the distance between the beam and the object and taking into account the nonlinearity of the excitation intensity profile, it is possible to obtain an oscillating response whose amplitude depends only on the distance to the surface of the object. Given the fact that the excitation beam is always near the structure of interest, it is possible to measure moving specimens. Here, we present tridimensional reconstructions of Arabidopsis Thaliana roots which are ~ 50 μm in length and ~ 5 μm in diameter. © 2018, Centro de Investigaciones en Laseres y Aplicaciones. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_18501168_v29_n1_p12_Zaza
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Fluorescence
Microscopy
Single particle tracking
spellingShingle Fluorescence
Microscopy
Single particle tracking
Zaza, C.
Gabriel, M.
Estrada, L.C.
3D microscopy by orbital radially modulated scan
topic_facet Fluorescence
Microscopy
Single particle tracking
description In this work, we present an alternative imaging method based on the orbital scanning of a laser excitation beam around the object of interest. This technology is capable of producing tridimensional images of fluorescent structures with nanometrical resolution in a few milliseconds. The method relies on the fact that when the excitation beam is near a fluorescent object, the emitted light from the object depends on the distance between its surface and the excitation beam. By modulating the distance between the beam and the object and taking into account the nonlinearity of the excitation intensity profile, it is possible to obtain an oscillating response whose amplitude depends only on the distance to the surface of the object. Given the fact that the excitation beam is always near the structure of interest, it is possible to measure moving specimens. Here, we present tridimensional reconstructions of Arabidopsis Thaliana roots which are ~ 50 μm in length and ~ 5 μm in diameter. © 2018, Centro de Investigaciones en Laseres y Aplicaciones. All rights reserved.
format JOUR
author Zaza, C.
Gabriel, M.
Estrada, L.C.
author_facet Zaza, C.
Gabriel, M.
Estrada, L.C.
author_sort Zaza, C.
title 3D microscopy by orbital radially modulated scan
title_short 3D microscopy by orbital radially modulated scan
title_full 3D microscopy by orbital radially modulated scan
title_fullStr 3D microscopy by orbital radially modulated scan
title_full_unstemmed 3D microscopy by orbital radially modulated scan
title_sort 3d microscopy by orbital radially modulated scan
url http://hdl.handle.net/20.500.12110/paper_18501168_v29_n1_p12_Zaza
work_keys_str_mv AT zazac 3dmicroscopybyorbitalradiallymodulatedscan
AT gabrielm 3dmicroscopybyorbitalradiallymodulatedscan
AT estradalc 3dmicroscopybyorbitalradiallymodulatedscan
_version_ 1782029301363769344

Ejemplares similares