Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies
Mathematical modelling approaches have become increasingly abundant in cancer research. Tumour infiltration extent and its spatial organization depend both on the tumour type and stage and on the bio-physicochemical characteristics of the microenvironment. This sets a complex scenario that often req...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_17579694_v10_n5_p325_Lujan |
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todo:paper_17579694_v10_n5_p325_Lujan2023-10-03T16:32:42Z Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies Luján, E. Soto, D. Rosito, M.S. Soba, A. Guerra, L.N. Calvo, J.C. Marshall, G. Suárez, C. collagen gel collagen type 1 adipocyte animal cell Article bland altman analysis cancer growth cancer infiltration cancer research cell differentiation cell proliferation computer model controlled study diffusion diffusion coefficient fractal analysis in vitro study intermethod comparison LM3 cell line (breast cancer) mathematical analysis mathematical model mathematical parameters mouse multicellular spheroid nonhuman priority journal proadipocyte qualitative analysis quantitative analysis reproducibility simulation thermodynamics three dimensional imaging tumor invasion tumor microenvironment tumor spheroid tumor volume velocity 3T3-L1 cell line animal biological model conditioned medium cytology experimental mammary neoplasm female pathology pathophysiology physiology three dimensional imaging tumor cell line tumor microenvironment tumor seeding 3T3-L1 Cells Adipocytes Animals Cell Line, Tumor Culture Media, Conditioned Female Imaging, Three-Dimensional Mammary Neoplasms, Experimental Mice Models, Biological Neoplasm Invasiveness Neoplasm Seeding Spheroids, Cellular Tumor Microenvironment Mathematical modelling approaches have become increasingly abundant in cancer research. Tumour infiltration extent and its spatial organization depend both on the tumour type and stage and on the bio-physicochemical characteristics of the microenvironment. This sets a complex scenario that often requires a multidisciplinary and individually adjusted approach. The ultimate goal of this work is to present an experimental/numerical combined method for the development of a three-dimensional mathematical model with the ability to reproduce the growth and infiltration patterns of a given avascular microtumour in response to different microenvironmental conditions. The model is based on a diffusion-convection reaction equation that considers logistic proliferation, volumetric growth, a rim of proliferative cells at the tumour surface, and invasion with diffusive and convective components. The parameter values of the model were fitted to experimental results while radial velocity and diffusion coefficients were made spatially variable in a case-specific way through the introduction of a shape function and a diffusion-limited-aggregation (DLA)-derived fractal matrix, respectively, according to the infiltration pattern observed. The in vitro model consists of multicellular tumour spheroids (MTSs) of an epithelial mammary tumour cell line (LM3) immersed in a collagen I gel matrix with a standard culture medium ("naive" matrix) or a conditioned medium from adipocytes or preadipocytes ("conditioned" matrix). It was experimentally determined that both adipocyte and preadipocyte conditioned media had the ability to change the MTS infiltration pattern from collective and laminar to an individual and atomized one. Numerical simulations were able to adequately reproduce qualitatively and quantitatively both kinds of infiltration patterns, which were determined by area quantification, analysis of fractal dimensions and lacunarity, and Bland-Altman analysis. These results suggest that the combined approach presented here could be established as a new framework with interesting potential applications at both the basic and clinical levels in the oncology area. © 2018 The Royal Society of Chemistry. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_17579694_v10_n5_p325_Lujan |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
collagen gel collagen type 1 adipocyte animal cell Article bland altman analysis cancer growth cancer infiltration cancer research cell differentiation cell proliferation computer model controlled study diffusion diffusion coefficient fractal analysis in vitro study intermethod comparison LM3 cell line (breast cancer) mathematical analysis mathematical model mathematical parameters mouse multicellular spheroid nonhuman priority journal proadipocyte qualitative analysis quantitative analysis reproducibility simulation thermodynamics three dimensional imaging tumor invasion tumor microenvironment tumor spheroid tumor volume velocity 3T3-L1 cell line animal biological model conditioned medium cytology experimental mammary neoplasm female pathology pathophysiology physiology three dimensional imaging tumor cell line tumor microenvironment tumor seeding 3T3-L1 Cells Adipocytes Animals Cell Line, Tumor Culture Media, Conditioned Female Imaging, Three-Dimensional Mammary Neoplasms, Experimental Mice Models, Biological Neoplasm Invasiveness Neoplasm Seeding Spheroids, Cellular Tumor Microenvironment |
| spellingShingle |
collagen gel collagen type 1 adipocyte animal cell Article bland altman analysis cancer growth cancer infiltration cancer research cell differentiation cell proliferation computer model controlled study diffusion diffusion coefficient fractal analysis in vitro study intermethod comparison LM3 cell line (breast cancer) mathematical analysis mathematical model mathematical parameters mouse multicellular spheroid nonhuman priority journal proadipocyte qualitative analysis quantitative analysis reproducibility simulation thermodynamics three dimensional imaging tumor invasion tumor microenvironment tumor spheroid tumor volume velocity 3T3-L1 cell line animal biological model conditioned medium cytology experimental mammary neoplasm female pathology pathophysiology physiology three dimensional imaging tumor cell line tumor microenvironment tumor seeding 3T3-L1 Cells Adipocytes Animals Cell Line, Tumor Culture Media, Conditioned Female Imaging, Three-Dimensional Mammary Neoplasms, Experimental Mice Models, Biological Neoplasm Invasiveness Neoplasm Seeding Spheroids, Cellular Tumor Microenvironment Luján, E. Soto, D. Rosito, M.S. Soba, A. Guerra, L.N. Calvo, J.C. Marshall, G. Suárez, C. Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies |
| topic_facet |
collagen gel collagen type 1 adipocyte animal cell Article bland altman analysis cancer growth cancer infiltration cancer research cell differentiation cell proliferation computer model controlled study diffusion diffusion coefficient fractal analysis in vitro study intermethod comparison LM3 cell line (breast cancer) mathematical analysis mathematical model mathematical parameters mouse multicellular spheroid nonhuman priority journal proadipocyte qualitative analysis quantitative analysis reproducibility simulation thermodynamics three dimensional imaging tumor invasion tumor microenvironment tumor spheroid tumor volume velocity 3T3-L1 cell line animal biological model conditioned medium cytology experimental mammary neoplasm female pathology pathophysiology physiology three dimensional imaging tumor cell line tumor microenvironment tumor seeding 3T3-L1 Cells Adipocytes Animals Cell Line, Tumor Culture Media, Conditioned Female Imaging, Three-Dimensional Mammary Neoplasms, Experimental Mice Models, Biological Neoplasm Invasiveness Neoplasm Seeding Spheroids, Cellular Tumor Microenvironment |
| description |
Mathematical modelling approaches have become increasingly abundant in cancer research. Tumour infiltration extent and its spatial organization depend both on the tumour type and stage and on the bio-physicochemical characteristics of the microenvironment. This sets a complex scenario that often requires a multidisciplinary and individually adjusted approach. The ultimate goal of this work is to present an experimental/numerical combined method for the development of a three-dimensional mathematical model with the ability to reproduce the growth and infiltration patterns of a given avascular microtumour in response to different microenvironmental conditions. The model is based on a diffusion-convection reaction equation that considers logistic proliferation, volumetric growth, a rim of proliferative cells at the tumour surface, and invasion with diffusive and convective components. The parameter values of the model were fitted to experimental results while radial velocity and diffusion coefficients were made spatially variable in a case-specific way through the introduction of a shape function and a diffusion-limited-aggregation (DLA)-derived fractal matrix, respectively, according to the infiltration pattern observed. The in vitro model consists of multicellular tumour spheroids (MTSs) of an epithelial mammary tumour cell line (LM3) immersed in a collagen I gel matrix with a standard culture medium ("naive" matrix) or a conditioned medium from adipocytes or preadipocytes ("conditioned" matrix). It was experimentally determined that both adipocyte and preadipocyte conditioned media had the ability to change the MTS infiltration pattern from collective and laminar to an individual and atomized one. Numerical simulations were able to adequately reproduce qualitatively and quantitatively both kinds of infiltration patterns, which were determined by area quantification, analysis of fractal dimensions and lacunarity, and Bland-Altman analysis. These results suggest that the combined approach presented here could be established as a new framework with interesting potential applications at both the basic and clinical levels in the oncology area. © 2018 The Royal Society of Chemistry. |
| format |
JOUR |
| author |
Luján, E. Soto, D. Rosito, M.S. Soba, A. Guerra, L.N. Calvo, J.C. Marshall, G. Suárez, C. |
| author_facet |
Luján, E. Soto, D. Rosito, M.S. Soba, A. Guerra, L.N. Calvo, J.C. Marshall, G. Suárez, C. |
| author_sort |
Luján, E. |
| title |
Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies |
| title_short |
Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies |
| title_full |
Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies |
| title_fullStr |
Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies |
| title_full_unstemmed |
Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies |
| title_sort |
microenvironmental influence on microtumour infiltration patterns: 3d-mathematical modelling supported by: in vitro studies |
| url |
http://hdl.handle.net/20.500.12110/paper_17579694_v10_n5_p325_Lujan |
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