Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster)

Octopamine (OA) underlies reinforcement during appetitive conditioning in the honey bee and fruit fly, acting via different subtypes of receptors. Recently, antibodies raised against a peptide sequence of one honey bee OA receptor, AmOA1, were used to study the distribution of these receptors in the...

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Autores principales:	Sinakevitch, I.T., Smith, A.N., Locatelli, F., Huerta, R., Bazhenov, M., Smith, B.H.
Formato:	JOUR
Materias:	Biogenic amine receptors G-protein receptors Learning and plasticity Octopamine Olfactory pathways 4 aminobutyric acid 4 aminobutyric acid antibody brain protein insect protein octopamine receptor 1 octopamine receptor 1 antibody protein antibody unclassified drug antenna (organ) Apis mellifera article cell labeling cell structure controlled study Drosophila melanogaster GABAergic system interneuron lateral antennoprotocerbral tract lateral protocerebral lobe medial antennoprotocerbral tract mushroom body nerve cell network neuroanatomy neuromodulation nonhuman olfactory nervous system olfactory receptor orthology protein determination protein expression protein localization sensory nerve cell
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_16625137_v7_nOCT_p_Sinakevitch
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_16625137_v7_nOCT_p_Sinakevitch2023-10-03T16:28:48Z Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster) Sinakevitch, I.T. Smith, A.N. Locatelli, F. Huerta, R. Bazhenov, M. Smith, B.H. Biogenic amine receptors G-protein receptors Learning and plasticity Octopamine Olfactory pathways 4 aminobutyric acid 4 aminobutyric acid antibody brain protein insect protein octopamine receptor 1 octopamine receptor 1 antibody protein antibody unclassified drug antenna (organ) Apis mellifera article cell labeling cell structure controlled study Drosophila melanogaster GABAergic system interneuron lateral antennoprotocerbral tract lateral protocerebral lobe medial antennoprotocerbral tract mushroom body nerve cell network neuroanatomy neuromodulation nonhuman olfactory nervous system olfactory receptor orthology protein determination protein expression protein localization sensory nerve cell Octopamine (OA) underlies reinforcement during appetitive conditioning in the honey bee and fruit fly, acting via different subtypes of receptors. Recently, antibodies raised against a peptide sequence of one honey bee OA receptor, AmOA1, were used to study the distribution of these receptors in the honey bee brain (Sinakevitch et al., 2011). These antibodies also recognize an isoform of the AmOA1 ortholog in the fruit fly (OAMB, mushroom body OA receptor). Here we describe in detail the distribution of AmOA1 receptors in different types of neurons in the honey bee and fruit fly antennal lobes. We integrate this information into a detailed anatomical analysis of olfactory receptor neurons (ORNs), uni- and multi-glomerular projection neurons (uPNs, and mPNs) and local interneurons (LNs) in glomeruli of the antennal lobe. These neurons were revealed by dye injection into the antennal nerve, antennal lobe, medial and lateral antenno-protocerbral tracts (m-APT and l-APT), and lateral protocerebral lobe (LPL) by use of labeled cell lines in the fruit fly or by staining with anti-GABA. We found that ORN receptor terminals and uPNs largely do not show immunostaining for AmOA1. About seventeen GABAergic mPNs leave the antennal lobe through the ml-APT and branch into the LPL. Many, but not all, mPNs show staining for AmOA1. AmOA1 receptors are also in glomeruli on GABAergic processes associated with LNs. The data suggest that in both species one important action of OA in the antennal lobe involves modulation of different types of inhibitory neurons via AmOA1 receptors. We integrated this new information into a model of circuitry within glomeruli of the antennal lobes of these species. © 2013 Sinakevitch, Smith, Locatelli, Huerta, Bazhenov and Smith. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16625137_v7_nOCT_p_Sinakevitch
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biogenic amine receptors G-protein receptors Learning and plasticity Octopamine Olfactory pathways 4 aminobutyric acid 4 aminobutyric acid antibody brain protein insect protein octopamine receptor 1 octopamine receptor 1 antibody protein antibody unclassified drug antenna (organ) Apis mellifera article cell labeling cell structure controlled study Drosophila melanogaster GABAergic system interneuron lateral antennoprotocerbral tract lateral protocerebral lobe medial antennoprotocerbral tract mushroom body nerve cell network neuroanatomy neuromodulation nonhuman olfactory nervous system olfactory receptor orthology protein determination protein expression protein localization sensory nerve cell
spellingShingle	Biogenic amine receptors G-protein receptors Learning and plasticity Octopamine Olfactory pathways 4 aminobutyric acid 4 aminobutyric acid antibody brain protein insect protein octopamine receptor 1 octopamine receptor 1 antibody protein antibody unclassified drug antenna (organ) Apis mellifera article cell labeling cell structure controlled study Drosophila melanogaster GABAergic system interneuron lateral antennoprotocerbral tract lateral protocerebral lobe medial antennoprotocerbral tract mushroom body nerve cell network neuroanatomy neuromodulation nonhuman olfactory nervous system olfactory receptor orthology protein determination protein expression protein localization sensory nerve cell Sinakevitch, I.T. Smith, A.N. Locatelli, F. Huerta, R. Bazhenov, M. Smith, B.H. Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster)
topic_facet	Biogenic amine receptors G-protein receptors Learning and plasticity Octopamine Olfactory pathways 4 aminobutyric acid 4 aminobutyric acid antibody brain protein insect protein octopamine receptor 1 octopamine receptor 1 antibody protein antibody unclassified drug antenna (organ) Apis mellifera article cell labeling cell structure controlled study Drosophila melanogaster GABAergic system interneuron lateral antennoprotocerbral tract lateral protocerebral lobe medial antennoprotocerbral tract mushroom body nerve cell network neuroanatomy neuromodulation nonhuman olfactory nervous system olfactory receptor orthology protein determination protein expression protein localization sensory nerve cell
description	Octopamine (OA) underlies reinforcement during appetitive conditioning in the honey bee and fruit fly, acting via different subtypes of receptors. Recently, antibodies raised against a peptide sequence of one honey bee OA receptor, AmOA1, were used to study the distribution of these receptors in the honey bee brain (Sinakevitch et al., 2011). These antibodies also recognize an isoform of the AmOA1 ortholog in the fruit fly (OAMB, mushroom body OA receptor). Here we describe in detail the distribution of AmOA1 receptors in different types of neurons in the honey bee and fruit fly antennal lobes. We integrate this information into a detailed anatomical analysis of olfactory receptor neurons (ORNs), uni- and multi-glomerular projection neurons (uPNs, and mPNs) and local interneurons (LNs) in glomeruli of the antennal lobe. These neurons were revealed by dye injection into the antennal nerve, antennal lobe, medial and lateral antenno-protocerbral tracts (m-APT and l-APT), and lateral protocerebral lobe (LPL) by use of labeled cell lines in the fruit fly or by staining with anti-GABA. We found that ORN receptor terminals and uPNs largely do not show immunostaining for AmOA1. About seventeen GABAergic mPNs leave the antennal lobe through the ml-APT and branch into the LPL. Many, but not all, mPNs show staining for AmOA1. AmOA1 receptors are also in glomeruli on GABAergic processes associated with LNs. The data suggest that in both species one important action of OA in the antennal lobe involves modulation of different types of inhibitory neurons via AmOA1 receptors. We integrated this new information into a model of circuitry within glomeruli of the antennal lobes of these species. © 2013 Sinakevitch, Smith, Locatelli, Huerta, Bazhenov and Smith.
format	JOUR
author	Sinakevitch, I.T. Smith, A.N. Locatelli, F. Huerta, R. Bazhenov, M. Smith, B.H.
author_facet	Sinakevitch, I.T. Smith, A.N. Locatelli, F. Huerta, R. Bazhenov, M. Smith, B.H.
author_sort	Sinakevitch, I.T.
title	Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster)
title_short	Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster)
title_full	Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster)
title_fullStr	Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster)
title_full_unstemmed	Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster)
title_sort	apis mellifera octopamine receptor 1 (amoa1) expression in antennal lobe networks of the honey bee (apis mellifera) and fruit fly (drosophila melanogaster)
url	http://hdl.handle.net/20.500.12110/paper_16625137_v7_nOCT_p_Sinakevitch
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Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster)

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