Photorelease of GABA with visible light using an inorganic caging group

We describe the selective photorelease of γ-amino butyric acid (GABA) with a novel caged-GABA compound that uses a ruthenium complex as photosensor. This compound ("RuBi-GABA") can be excited with visible wavelengths, providing greater tissue penetration, less photo-toxicity, and faster ph...

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Autores principales: Verde, E.M.R., Zayat, L., Etchenique, R., Yuste, R.
Formato: JOUR
Materias:
GABA
Inhibition
Photorelease
Ruthenium
Uncaging
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_16625110_v2_nAUG_p_Verde
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_16625110_v2_nAUG_p_Verde
record_format dspace
spelling todo:paper_16625110_v2_nAUG_p_Verde2023-10-03T16:28:47Z Photorelease of GABA with visible light using an inorganic caging group Verde, E.M.R. Zayat, L. Etchenique, R. Yuste, R. GABA Inhibition Photorelease Ruthenium Uncaging We describe the selective photorelease of γ-amino butyric acid (GABA) with a novel caged-GABA compound that uses a ruthenium complex as photosensor. This compound ("RuBi-GABA") can be excited with visible wavelengths, providing greater tissue penetration, less photo-toxicity, and faster photorelease kinetics than currently used UV light-sensitive caged compounds. Using pyramidal neurons from neocortical brain slices, we show that RuBi-GABA uncaging induces GABA-A receptor-mediated responses, has no detectable side effects on endogenous GABAergic and glutamatergic receptors and generates responses with kinetics and spatial resolution comparable to the best caged GABA compounds presently available. Finally, we illustrate two potential applications of RuBi-GABA uncaging: GABA receptor mapping, and optical silencing of neuronal firing. © 2008 Rial Verde, Zayat, Etchenique and Yuste. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16625110_v2_nAUG_p_Verde
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic GABA
Inhibition
Photorelease
Ruthenium
Uncaging
spellingShingle GABA
Inhibition
Photorelease
Ruthenium
Uncaging
Verde, E.M.R.
Zayat, L.
Etchenique, R.
Yuste, R.
Photorelease of GABA with visible light using an inorganic caging group
topic_facet GABA
Inhibition
Photorelease
Ruthenium
Uncaging
description We describe the selective photorelease of γ-amino butyric acid (GABA) with a novel caged-GABA compound that uses a ruthenium complex as photosensor. This compound ("RuBi-GABA") can be excited with visible wavelengths, providing greater tissue penetration, less photo-toxicity, and faster photorelease kinetics than currently used UV light-sensitive caged compounds. Using pyramidal neurons from neocortical brain slices, we show that RuBi-GABA uncaging induces GABA-A receptor-mediated responses, has no detectable side effects on endogenous GABAergic and glutamatergic receptors and generates responses with kinetics and spatial resolution comparable to the best caged GABA compounds presently available. Finally, we illustrate two potential applications of RuBi-GABA uncaging: GABA receptor mapping, and optical silencing of neuronal firing. © 2008 Rial Verde, Zayat, Etchenique and Yuste.
format JOUR
author Verde, E.M.R.
Zayat, L.
Etchenique, R.
Yuste, R.
author_facet Verde, E.M.R.
Zayat, L.
Etchenique, R.
Yuste, R.
author_sort Verde, E.M.R.
title Photorelease of GABA with visible light using an inorganic caging group
title_short Photorelease of GABA with visible light using an inorganic caging group
title_full Photorelease of GABA with visible light using an inorganic caging group
title_fullStr Photorelease of GABA with visible light using an inorganic caging group
title_full_unstemmed Photorelease of GABA with visible light using an inorganic caging group
title_sort photorelease of gaba with visible light using an inorganic caging group
url http://hdl.handle.net/20.500.12110/paper_16625110_v2_nAUG_p_Verde
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