Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole

Pseudocercospora griseola, an anamorph of Mycosphaerella, causes Angular Leaf Spot (ALS). The mycelia and conidia from P. griseola are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in P. griseola f. mesoamericana isolate T4, a highly pi...

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Autores principales:	Bárcena, A., Petroselli, G., Velasquez, S.M., Estévez, J.M., Erra-Balsells, R., Balatti, P.A., Saparrat, M.C.N.
Formato:	JOUR
Materias:	FTIR Melanin Microscopy Phaseolus vulgaris Pseudocercospora griseola f. mesoamericana Tricyclazole UV-MALDI-TOF MS Fungi Mycosphaerella Pseudocercospora
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1617416X_v14_n9_p_Barcena
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_1617416X_v14_n9_p_Barcena
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spelling	todo:paper_1617416X_v14_n9_p_Barcena2023-10-03T16:28:24Z Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole Bárcena, A. Petroselli, G. Velasquez, S.M. Estévez, J.M. Erra-Balsells, R. Balatti, P.A. Saparrat, M.C.N. FTIR Melanin Microscopy Phaseolus vulgaris Pseudocercospora griseola f. mesoamericana Tricyclazole UV-MALDI-TOF MS Fungi Mycosphaerella Phaseolus vulgaris Pseudocercospora Pseudocercospora griseola, an anamorph of Mycosphaerella, causes Angular Leaf Spot (ALS). The mycelia and conidia from P. griseola are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in P. griseola f. mesoamericana isolate T4, a highly pigmented fungus, intermediary compounds as a result of the inhibition of melanin synthesis by tricyclazole, and to analyze at the structural level the localization of these dark pigments. The main metabolites were analyzed using ultraviolet matrix-assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Tricyclazole affected P. griseola f. mesoamericana in several different ways. The most evident effect was the reduction of melanin synthesis, and therefore diffusible shunt products were found and identified. Flaviolin was the main intermediate metabolite found in cultures supplemented with tricyclazole. This inhibitor, which affected pigmentation and the cell wall structure of mycelium, revealed macroscopically by the reduction in growth, decreased the stratification and deposition of melanin in the hyphal wall. These results suggest a possible role of tricyclazole to control ALS. © 2015, German Mycological Society and Springer-Verlag Berlin Heidelberg. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1617416X_v14_n9_p_Barcena
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	FTIR Melanin Microscopy Phaseolus vulgaris Pseudocercospora griseola f. mesoamericana Tricyclazole UV-MALDI-TOF MS Fungi Mycosphaerella Phaseolus vulgaris Pseudocercospora
spellingShingle	FTIR Melanin Microscopy Phaseolus vulgaris Pseudocercospora griseola f. mesoamericana Tricyclazole UV-MALDI-TOF MS Fungi Mycosphaerella Phaseolus vulgaris Pseudocercospora Bárcena, A. Petroselli, G. Velasquez, S.M. Estévez, J.M. Erra-Balsells, R. Balatti, P.A. Saparrat, M.C.N. Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole
topic_facet	FTIR Melanin Microscopy Phaseolus vulgaris Pseudocercospora griseola f. mesoamericana Tricyclazole UV-MALDI-TOF MS Fungi Mycosphaerella Phaseolus vulgaris Pseudocercospora
description	Pseudocercospora griseola, an anamorph of Mycosphaerella, causes Angular Leaf Spot (ALS). The mycelia and conidia from P. griseola are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in P. griseola f. mesoamericana isolate T4, a highly pigmented fungus, intermediary compounds as a result of the inhibition of melanin synthesis by tricyclazole, and to analyze at the structural level the localization of these dark pigments. The main metabolites were analyzed using ultraviolet matrix-assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Tricyclazole affected P. griseola f. mesoamericana in several different ways. The most evident effect was the reduction of melanin synthesis, and therefore diffusible shunt products were found and identified. Flaviolin was the main intermediate metabolite found in cultures supplemented with tricyclazole. This inhibitor, which affected pigmentation and the cell wall structure of mycelium, revealed macroscopically by the reduction in growth, decreased the stratification and deposition of melanin in the hyphal wall. These results suggest a possible role of tricyclazole to control ALS. © 2015, German Mycological Society and Springer-Verlag Berlin Heidelberg.
format	JOUR
author	Bárcena, A. Petroselli, G. Velasquez, S.M. Estévez, J.M. Erra-Balsells, R. Balatti, P.A. Saparrat, M.C.N.
author_facet	Bárcena, A. Petroselli, G. Velasquez, S.M. Estévez, J.M. Erra-Balsells, R. Balatti, P.A. Saparrat, M.C.N.
author_sort	Bárcena, A.
title	Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole
title_short	Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole
title_full	Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole
title_fullStr	Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole
title_full_unstemmed	Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole
title_sort	response of the fungus pseudocercospora griseola f. mesoamericana to tricyclazole
url	http://hdl.handle.net/20.500.12110/paper_1617416X_v14_n9_p_Barcena
work_keys_str_mv	AT barcenaa responseofthefunguspseudocercosporagriseolafmesoamericanatotricyclazole AT petrosellig responseofthefunguspseudocercosporagriseolafmesoamericanatotricyclazole AT velasquezsm responseofthefunguspseudocercosporagriseolafmesoamericanatotricyclazole AT estevezjm responseofthefunguspseudocercosporagriseolafmesoamericanatotricyclazole AT errabalsellsr responseofthefunguspseudocercosporagriseolafmesoamericanatotricyclazole AT balattipa responseofthefunguspseudocercosporagriseolafmesoamericanatotricyclazole AT saparratmcn responseofthefunguspseudocercosporagriseolafmesoamericanatotricyclazole
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Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole

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