Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus

Eucalyptus globulus is the most commonly planted hardwood species for pulpwood in temperate regions. We aimed to develop and characterize functional molecular markers for population genetic analyses and molecular breeding in this model tree species. Public expressed sequence tag (EST) databases were...

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Autores principales: Acuña, C.V., Fernandez, P., Villalba, P.V., García, M.N., Hopp, H.E., Marcucci Poltri, S.N.
Formato: JOUR
Materias:
EST-SSR
Eucalyptus globulus
Functional diversity
Polymorphism
Transferability
Eucalyptus
Eucalyptus camaldulensis
Eucalyptus dunnii
Eucalyptus grandis
Eucalyptus saligna
Eucalyptus tereticornis
Eucalyptus viminalis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_16142942_v8_n2_p289_Acuna
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_16142942_v8_n2_p289_Acuna2023-10-03T16:28:11Z Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus Acuña, C.V. Fernandez, P. Villalba, P.V. García, M.N. Hopp, H.E. Marcucci Poltri, S.N. EST-SSR Eucalyptus globulus Functional diversity Polymorphism Transferability Eucalyptus Eucalyptus camaldulensis Eucalyptus dunnii Eucalyptus globulus Eucalyptus grandis Eucalyptus saligna Eucalyptus tereticornis Eucalyptus viminalis Eucalyptus globulus is the most commonly planted hardwood species for pulpwood in temperate regions. We aimed to develop and characterize functional molecular markers for population genetic analyses and molecular breeding in this model tree species. Public expressed sequence tag (EST) databases were screened for nonredundant sequences to predict putative gene functions and to discover simple sequence repeats (EST-SSRs), which were then validated in E. globulus and six other Eucalyptus species. A total of 4,924 nonredundant sequences were identified from 12,690 updated E. globulus ESTs. Approximately 19. 3% (952) were unigenes and contained 1,140 EST-SSR markers, which were mainly trimeric (58. 6%). A set of 979 primers for putative SSR markers was designed after bioinformatic analysis. The predicted functions of these ESTs containing SSR were classified according to their gene ontology (GO) categories (biological process, molecular function, and cellular component). GO categories were assigned to 226 ESTs (30. 2%). Most ESTs containing SSR (78. 7%) had significant matches (E ≤ 10 -5) with the nonredundant protein database using BLASTX. From a set of 56 random primer pairs, 37 could be validated in eight E. globulus genotypes and were also tested for cross-transferability to other six Eucalyptus species (Eucalyptus grandis, Eucalyptus saligna, Eucalyptus dunnii, Eucalyptus viminalis, Eucalyptus camaldulensis, Eucalyptus tereticornis). Seventeen polymorphic EST-SSR markers for E. globulus were evaluated in 60 unrelated trees, being representative of the species' natural distribution. As a result, six highly informative markers were proposed for genetic diversity analyses, fingerprinting, and comparative population studies, between different species of E. globulus. © 2011 Springer-Verlag. Fil:Acuña, C.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marcucci Poltri, S.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16142942_v8_n2_p289_Acuna
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic EST-SSR
Eucalyptus globulus
Functional diversity
Polymorphism
Transferability
Eucalyptus
Eucalyptus camaldulensis
Eucalyptus dunnii
Eucalyptus globulus
Eucalyptus grandis
Eucalyptus saligna
Eucalyptus tereticornis
Eucalyptus viminalis
spellingShingle EST-SSR
Eucalyptus globulus
Functional diversity
Polymorphism
Transferability
Eucalyptus
Eucalyptus camaldulensis
Eucalyptus dunnii
Eucalyptus globulus
Eucalyptus grandis
Eucalyptus saligna
Eucalyptus tereticornis
Eucalyptus viminalis
Acuña, C.V.
Fernandez, P.
Villalba, P.V.
García, M.N.
Hopp, H.E.
Marcucci Poltri, S.N.
Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus
topic_facet EST-SSR
Eucalyptus globulus
Functional diversity
Polymorphism
Transferability
Eucalyptus
Eucalyptus camaldulensis
Eucalyptus dunnii
Eucalyptus globulus
Eucalyptus grandis
Eucalyptus saligna
Eucalyptus tereticornis
Eucalyptus viminalis
description Eucalyptus globulus is the most commonly planted hardwood species for pulpwood in temperate regions. We aimed to develop and characterize functional molecular markers for population genetic analyses and molecular breeding in this model tree species. Public expressed sequence tag (EST) databases were screened for nonredundant sequences to predict putative gene functions and to discover simple sequence repeats (EST-SSRs), which were then validated in E. globulus and six other Eucalyptus species. A total of 4,924 nonredundant sequences were identified from 12,690 updated E. globulus ESTs. Approximately 19. 3% (952) were unigenes and contained 1,140 EST-SSR markers, which were mainly trimeric (58. 6%). A set of 979 primers for putative SSR markers was designed after bioinformatic analysis. The predicted functions of these ESTs containing SSR were classified according to their gene ontology (GO) categories (biological process, molecular function, and cellular component). GO categories were assigned to 226 ESTs (30. 2%). Most ESTs containing SSR (78. 7%) had significant matches (E ≤ 10 -5) with the nonredundant protein database using BLASTX. From a set of 56 random primer pairs, 37 could be validated in eight E. globulus genotypes and were also tested for cross-transferability to other six Eucalyptus species (Eucalyptus grandis, Eucalyptus saligna, Eucalyptus dunnii, Eucalyptus viminalis, Eucalyptus camaldulensis, Eucalyptus tereticornis). Seventeen polymorphic EST-SSR markers for E. globulus were evaluated in 60 unrelated trees, being representative of the species' natural distribution. As a result, six highly informative markers were proposed for genetic diversity analyses, fingerprinting, and comparative population studies, between different species of E. globulus. © 2011 Springer-Verlag.
format JOUR
author Acuña, C.V.
Fernandez, P.
Villalba, P.V.
García, M.N.
Hopp, H.E.
Marcucci Poltri, S.N.
author_facet Acuña, C.V.
Fernandez, P.
Villalba, P.V.
García, M.N.
Hopp, H.E.
Marcucci Poltri, S.N.
author_sort Acuña, C.V.
title Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus
title_short Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus
title_full Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus
title_fullStr Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus
title_full_unstemmed Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus
title_sort discovery, validation, and in silico functional characterization of est-ssr markers in eucalyptus globulus
url http://hdl.handle.net/20.500.12110/paper_16142942_v8_n2_p289_Acuna
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