Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster

The standard version of the wing somatic mutation and recombination test (SMART) in Drosophila melanogaster was employed in order to evaluate the genotoxic potential of metronidazole (MTZ) as a function of exposure concentration. MTZ was administered by chronic feeding of 3-day-old larvae with the p...

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Autores principales:	Palermo, A.M., Mudry, M.D.
Formato:	JOUR
Materias:	Drosophila Genotoxicity Loss of heterozygosity Metronidazole (MTZ) Wing spot assay metronidazole article carcinogenesis chromosome aberration controlled study DNA damage Drosophila melanogaster gene conversion genotoxicity heterozygosity loss mitotic recombination nonhuman phenotype point mutation priority journal progeny somatic cell somatic mutation Animals Anti-Infective Agents DNA Damage Dose-Response Relationship, Drug Larva Metronidazole Mutagenicity Tests Mutagens Wing
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15748863_v8_n3_p195_Palermo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_15748863_v8_n3_p195_Palermo
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spelling	todo:paper_15748863_v8_n3_p195_Palermo2023-10-03T16:27:37Z Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster Palermo, A.M. Mudry, M.D. Drosophila Genotoxicity Loss of heterozygosity Metronidazole (MTZ) Wing spot assay metronidazole article carcinogenesis chromosome aberration controlled study DNA damage Drosophila melanogaster gene conversion genotoxicity heterozygosity loss mitotic recombination nonhuman phenotype point mutation priority journal progeny somatic cell somatic mutation Animals Anti-Infective Agents DNA Damage Dose-Response Relationship, Drug Drosophila melanogaster Larva Metronidazole Mutagenicity Tests Mutagens Wing The standard version of the wing somatic mutation and recombination test (SMART) in Drosophila melanogaster was employed in order to evaluate the genotoxic potential of metronidazole (MTZ) as a function of exposure concentration. MTZ was administered by chronic feeding of 3-day-old larvae with the parenteral solution at 0, 500, 1000 and 2000 μg/ml until pupation. The marker-heterozygous progeny (mwh+/+flr3) with phenotypically wild-type wings was analyzed. Non significant differences were found between control and each MTZ concentration tested for single small spots (SSS) frequencies. Large single spots (LSS) and twin spots (TS) were significantly increased with the higher dose. MTZ treatments with 1000 and 2000 μg/ml also significantly increased the frequency of Total spots. These findings suggest that MTZ is genotoxic in the present experimental conditions and induces recombinagenesis and/or gene conversion, two major mechanisms that cause loss of heterocigosity and could play an important role in tumorigenesis and carcinogenesis processes. © 2013 Bentham Science Publishers. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15748863_v8_n3_p195_Palermo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Drosophila Genotoxicity Loss of heterozygosity Metronidazole (MTZ) Wing spot assay metronidazole article carcinogenesis chromosome aberration controlled study DNA damage Drosophila melanogaster gene conversion genotoxicity heterozygosity loss mitotic recombination nonhuman phenotype point mutation priority journal progeny somatic cell somatic mutation Animals Anti-Infective Agents DNA Damage Dose-Response Relationship, Drug Drosophila melanogaster Larva Metronidazole Mutagenicity Tests Mutagens Wing
spellingShingle	Drosophila Genotoxicity Loss of heterozygosity Metronidazole (MTZ) Wing spot assay metronidazole article carcinogenesis chromosome aberration controlled study DNA damage Drosophila melanogaster gene conversion genotoxicity heterozygosity loss mitotic recombination nonhuman phenotype point mutation priority journal progeny somatic cell somatic mutation Animals Anti-Infective Agents DNA Damage Dose-Response Relationship, Drug Drosophila melanogaster Larva Metronidazole Mutagenicity Tests Mutagens Wing Palermo, A.M. Mudry, M.D. Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster
topic_facet	Drosophila Genotoxicity Loss of heterozygosity Metronidazole (MTZ) Wing spot assay metronidazole article carcinogenesis chromosome aberration controlled study DNA damage Drosophila melanogaster gene conversion genotoxicity heterozygosity loss mitotic recombination nonhuman phenotype point mutation priority journal progeny somatic cell somatic mutation Animals Anti-Infective Agents DNA Damage Dose-Response Relationship, Drug Drosophila melanogaster Larva Metronidazole Mutagenicity Tests Mutagens Wing
description	The standard version of the wing somatic mutation and recombination test (SMART) in Drosophila melanogaster was employed in order to evaluate the genotoxic potential of metronidazole (MTZ) as a function of exposure concentration. MTZ was administered by chronic feeding of 3-day-old larvae with the parenteral solution at 0, 500, 1000 and 2000 μg/ml until pupation. The marker-heterozygous progeny (mwh+/+flr3) with phenotypically wild-type wings was analyzed. Non significant differences were found between control and each MTZ concentration tested for single small spots (SSS) frequencies. Large single spots (LSS) and twin spots (TS) were significantly increased with the higher dose. MTZ treatments with 1000 and 2000 μg/ml also significantly increased the frequency of Total spots. These findings suggest that MTZ is genotoxic in the present experimental conditions and induces recombinagenesis and/or gene conversion, two major mechanisms that cause loss of heterocigosity and could play an important role in tumorigenesis and carcinogenesis processes. © 2013 Bentham Science Publishers.
format	JOUR
author	Palermo, A.M. Mudry, M.D.
author_facet	Palermo, A.M. Mudry, M.D.
author_sort	Palermo, A.M.
title	Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster
title_short	Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster
title_full	Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster
title_fullStr	Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster
title_full_unstemmed	Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster
title_sort	metronidazole induced dna damage in somatic cells of drosophila melanogaster
url	http://hdl.handle.net/20.500.12110/paper_15748863_v8_n3_p195_Palermo
work_keys_str_mv	AT palermoam metronidazoleinduceddnadamageinsomaticcellsofdrosophilamelanogaster AT mudrymd metronidazoleinduceddnadamageinsomaticcellsofdrosophilamelanogaster
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Metronidazole induced DNA damage in somatic cells of Drosophila melanogaster

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