Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection
In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescen...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_15726495_v681_n2_p381_Vitale |
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todo:paper_15726495_v681_n2_p381_Vitale2023-10-03T16:27:23Z Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection Vitale, A.A. Ferrari, C.C. Aldana, H. Affanni, J.M. melatonin melatonin animal tissue article controlled study drug determination fluorometry high performance liquid chromatography male nonhuman pineal body priority journal rat technique In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescence detection. This method is sensitive enough for detecting melatonin in a single pineal gland with a detection limit of 3 pg/mg tissue. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15726495_v681_n2_p381_Vitale |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
melatonin melatonin animal tissue article controlled study drug determination fluorometry high performance liquid chromatography male nonhuman pineal body priority journal rat technique |
| spellingShingle |
melatonin melatonin animal tissue article controlled study drug determination fluorometry high performance liquid chromatography male nonhuman pineal body priority journal rat technique Vitale, A.A. Ferrari, C.C. Aldana, H. Affanni, J.M. Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection |
| topic_facet |
melatonin melatonin animal tissue article controlled study drug determination fluorometry high performance liquid chromatography male nonhuman pineal body priority journal rat technique |
| description |
In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescence detection. This method is sensitive enough for detecting melatonin in a single pineal gland with a detection limit of 3 pg/mg tissue. |
| format |
JOUR |
| author |
Vitale, A.A. Ferrari, C.C. Aldana, H. Affanni, J.M. |
| author_facet |
Vitale, A.A. Ferrari, C.C. Aldana, H. Affanni, J.M. |
| author_sort |
Vitale, A.A. |
| title |
Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection |
| title_short |
Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection |
| title_full |
Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection |
| title_fullStr |
Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection |
| title_full_unstemmed |
Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection |
| title_sort |
highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection |
| url |
http://hdl.handle.net/20.500.12110/paper_15726495_v681_n2_p381_Vitale |
| work_keys_str_mv |
AT vitaleaa highlysensitivemethodforthedeterminationofmelatoninbynormalphasehighperformanceliquidchromatographywithfluorometricdetection AT ferraricc highlysensitivemethodforthedeterminationofmelatoninbynormalphasehighperformanceliquidchromatographywithfluorometricdetection AT aldanah highlysensitivemethodforthedeterminationofmelatoninbynormalphasehighperformanceliquidchromatographywithfluorometricdetection AT affannijm highlysensitivemethodforthedeterminationofmelatoninbynormalphasehighperformanceliquidchromatographywithfluorometricdetection |
| _version_ |
1807324431581708288 |