Cell membrane electroporation modeling: A multiphysics approach
Electroporation-based techniques, i.e. techniques based on the perturbation of the cell membrane through the application of electric pulses, are widely used at present in medicine and biotechnology. However, the electric pulse - cell membrane interaction is not yet completely understood neither expl...
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todo:paper_15675394_v124_n_p28_Goldberg2023-10-03T16:26:39Z Cell membrane electroporation modeling: A multiphysics approach Goldberg, E. Suárez, C. Alfonso, M. Marchese, J. Soba, A. Marshall, G. Electrochemotherapy Electroporation Ion transport Mathematical modeling Membrane deformation Bioelectric potentials Cells Chlorine compounds Electric fields Ions Mathematical models Maxwell equations Membranes Poisson equation Cell membrane interactions Electrochemotherapy Electroporation Ion transports Membrane electroporation Membrane permeabilization Nernst-Planck equations Transmembrane potentials Cytology Article biotechnology cell membrane cell volume CHO cell line electrochemotherapy electroporation erythrocyte ion transport lipid vesicle mathematical model membrane potential nonhuman animal biological model cell membrane Cricetulus electroporation erythrocyte deformability metabolism physiology procedures calcium chloride Animals Calcium Cell Membrane Chlorides CHO Cells Cricetulus Electroporation Erythrocyte Deformability Erythrocytes Ion Transport Membrane Potentials Models, Biological Electroporation-based techniques, i.e. techniques based on the perturbation of the cell membrane through the application of electric pulses, are widely used at present in medicine and biotechnology. However, the electric pulse - cell membrane interaction is not yet completely understood neither explicitly formalized. Here we introduce a Multiphysics (MP) model describing electric pulse - cell membrane interaction consisting on the Poisson equation for the electric field, the Nernst-Planck equations for ion transport (protons, hydroxides, sodium or calcium, and chloride), the Maxwell tensor and mechanical equilibrium equation for membrane deformations (with an explicit discretization of the cell membrane), and the Smoluchowski equation for membrane permeabilization. The MP model predicts that during the application of an electric pulse to a spherical cell an elastic deformation of its membrane takes place affecting the induced transmembrane potential, the pore creation dynamics and the ionic transport. Moreover, the coincidence among maximum membrane deformation, maximum pore aperture, and maximum ion uptake is predicted. Such behavior has been corroborated experimentally by previously published results in red blood and CHO cells as well as in supramolecular lipid vesicles. © 2018 JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15675394_v124_n_p28_Goldberg |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Electrochemotherapy Electroporation Ion transport Mathematical modeling Membrane deformation Bioelectric potentials Cells Chlorine compounds Electric fields Ions Mathematical models Maxwell equations Membranes Poisson equation Cell membrane interactions Electrochemotherapy Electroporation Ion transports Membrane electroporation Membrane permeabilization Nernst-Planck equations Transmembrane potentials Cytology Article biotechnology cell membrane cell volume CHO cell line electrochemotherapy electroporation erythrocyte ion transport lipid vesicle mathematical model membrane potential nonhuman animal biological model cell membrane Cricetulus electroporation erythrocyte deformability metabolism physiology procedures calcium chloride Animals Calcium Cell Membrane Chlorides CHO Cells Cricetulus Electroporation Erythrocyte Deformability Erythrocytes Ion Transport Membrane Potentials Models, Biological |
spellingShingle |
Electrochemotherapy Electroporation Ion transport Mathematical modeling Membrane deformation Bioelectric potentials Cells Chlorine compounds Electric fields Ions Mathematical models Maxwell equations Membranes Poisson equation Cell membrane interactions Electrochemotherapy Electroporation Ion transports Membrane electroporation Membrane permeabilization Nernst-Planck equations Transmembrane potentials Cytology Article biotechnology cell membrane cell volume CHO cell line electrochemotherapy electroporation erythrocyte ion transport lipid vesicle mathematical model membrane potential nonhuman animal biological model cell membrane Cricetulus electroporation erythrocyte deformability metabolism physiology procedures calcium chloride Animals Calcium Cell Membrane Chlorides CHO Cells Cricetulus Electroporation Erythrocyte Deformability Erythrocytes Ion Transport Membrane Potentials Models, Biological Goldberg, E. Suárez, C. Alfonso, M. Marchese, J. Soba, A. Marshall, G. Cell membrane electroporation modeling: A multiphysics approach |
topic_facet |
Electrochemotherapy Electroporation Ion transport Mathematical modeling Membrane deformation Bioelectric potentials Cells Chlorine compounds Electric fields Ions Mathematical models Maxwell equations Membranes Poisson equation Cell membrane interactions Electrochemotherapy Electroporation Ion transports Membrane electroporation Membrane permeabilization Nernst-Planck equations Transmembrane potentials Cytology Article biotechnology cell membrane cell volume CHO cell line electrochemotherapy electroporation erythrocyte ion transport lipid vesicle mathematical model membrane potential nonhuman animal biological model cell membrane Cricetulus electroporation erythrocyte deformability metabolism physiology procedures calcium chloride Animals Calcium Cell Membrane Chlorides CHO Cells Cricetulus Electroporation Erythrocyte Deformability Erythrocytes Ion Transport Membrane Potentials Models, Biological |
description |
Electroporation-based techniques, i.e. techniques based on the perturbation of the cell membrane through the application of electric pulses, are widely used at present in medicine and biotechnology. However, the electric pulse - cell membrane interaction is not yet completely understood neither explicitly formalized. Here we introduce a Multiphysics (MP) model describing electric pulse - cell membrane interaction consisting on the Poisson equation for the electric field, the Nernst-Planck equations for ion transport (protons, hydroxides, sodium or calcium, and chloride), the Maxwell tensor and mechanical equilibrium equation for membrane deformations (with an explicit discretization of the cell membrane), and the Smoluchowski equation for membrane permeabilization. The MP model predicts that during the application of an electric pulse to a spherical cell an elastic deformation of its membrane takes place affecting the induced transmembrane potential, the pore creation dynamics and the ionic transport. Moreover, the coincidence among maximum membrane deformation, maximum pore aperture, and maximum ion uptake is predicted. Such behavior has been corroborated experimentally by previously published results in red blood and CHO cells as well as in supramolecular lipid vesicles. © 2018 |
format |
JOUR |
author |
Goldberg, E. Suárez, C. Alfonso, M. Marchese, J. Soba, A. Marshall, G. |
author_facet |
Goldberg, E. Suárez, C. Alfonso, M. Marchese, J. Soba, A. Marshall, G. |
author_sort |
Goldberg, E. |
title |
Cell membrane electroporation modeling: A multiphysics approach |
title_short |
Cell membrane electroporation modeling: A multiphysics approach |
title_full |
Cell membrane electroporation modeling: A multiphysics approach |
title_fullStr |
Cell membrane electroporation modeling: A multiphysics approach |
title_full_unstemmed |
Cell membrane electroporation modeling: A multiphysics approach |
title_sort |
cell membrane electroporation modeling: a multiphysics approach |
url |
http://hdl.handle.net/20.500.12110/paper_15675394_v124_n_p28_Goldberg |
work_keys_str_mv |
AT goldberge cellmembraneelectroporationmodelingamultiphysicsapproach AT suarezc cellmembraneelectroporationmodelingamultiphysicsapproach AT alfonsom cellmembraneelectroporationmodelingamultiphysicsapproach AT marchesej cellmembraneelectroporationmodelingamultiphysicsapproach AT sobaa cellmembraneelectroporationmodelingamultiphysicsapproach AT marshallg cellmembraneelectroporationmodelingamultiphysicsapproach |
_version_ |
1807323195308507136 |