Cell membrane electroporation modeling: A multiphysics approach

Electroporation-based techniques, i.e. techniques based on the perturbation of the cell membrane through the application of electric pulses, are widely used at present in medicine and biotechnology. However, the electric pulse - cell membrane interaction is not yet completely understood neither expl...

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Autores principales: Goldberg, E., Suárez, C., Alfonso, M., Marchese, J., Soba, A., Marshall, G.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15675394_v124_n_p28_Goldberg
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spelling todo:paper_15675394_v124_n_p28_Goldberg2023-10-03T16:26:39Z Cell membrane electroporation modeling: A multiphysics approach Goldberg, E. Suárez, C. Alfonso, M. Marchese, J. Soba, A. Marshall, G. Electrochemotherapy Electroporation Ion transport Mathematical modeling Membrane deformation Bioelectric potentials Cells Chlorine compounds Electric fields Ions Mathematical models Maxwell equations Membranes Poisson equation Cell membrane interactions Electrochemotherapy Electroporation Ion transports Membrane electroporation Membrane permeabilization Nernst-Planck equations Transmembrane potentials Cytology Article biotechnology cell membrane cell volume CHO cell line electrochemotherapy electroporation erythrocyte ion transport lipid vesicle mathematical model membrane potential nonhuman animal biological model cell membrane Cricetulus electroporation erythrocyte deformability metabolism physiology procedures calcium chloride Animals Calcium Cell Membrane Chlorides CHO Cells Cricetulus Electroporation Erythrocyte Deformability Erythrocytes Ion Transport Membrane Potentials Models, Biological Electroporation-based techniques, i.e. techniques based on the perturbation of the cell membrane through the application of electric pulses, are widely used at present in medicine and biotechnology. However, the electric pulse - cell membrane interaction is not yet completely understood neither explicitly formalized. Here we introduce a Multiphysics (MP) model describing electric pulse - cell membrane interaction consisting on the Poisson equation for the electric field, the Nernst-Planck equations for ion transport (protons, hydroxides, sodium or calcium, and chloride), the Maxwell tensor and mechanical equilibrium equation for membrane deformations (with an explicit discretization of the cell membrane), and the Smoluchowski equation for membrane permeabilization. The MP model predicts that during the application of an electric pulse to a spherical cell an elastic deformation of its membrane takes place affecting the induced transmembrane potential, the pore creation dynamics and the ionic transport. Moreover, the coincidence among maximum membrane deformation, maximum pore aperture, and maximum ion uptake is predicted. Such behavior has been corroborated experimentally by previously published results in red blood and CHO cells as well as in supramolecular lipid vesicles. © 2018 JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15675394_v124_n_p28_Goldberg
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Electrochemotherapy
Electroporation
Ion transport
Mathematical modeling
Membrane deformation
Bioelectric potentials
Cells
Chlorine compounds
Electric fields
Ions
Mathematical models
Maxwell equations
Membranes
Poisson equation
Cell membrane interactions
Electrochemotherapy
Electroporation
Ion transports
Membrane electroporation
Membrane permeabilization
Nernst-Planck equations
Transmembrane potentials
Cytology
Article
biotechnology
cell membrane
cell volume
CHO cell line
electrochemotherapy
electroporation
erythrocyte
ion transport
lipid vesicle
mathematical model
membrane potential
nonhuman
animal
biological model
cell membrane
Cricetulus
electroporation
erythrocyte deformability
metabolism
physiology
procedures
calcium
chloride
Animals
Calcium
Cell Membrane
Chlorides
CHO Cells
Cricetulus
Electroporation
Erythrocyte Deformability
Erythrocytes
Ion Transport
Membrane Potentials
Models, Biological
spellingShingle Electrochemotherapy
Electroporation
Ion transport
Mathematical modeling
Membrane deformation
Bioelectric potentials
Cells
Chlorine compounds
Electric fields
Ions
Mathematical models
Maxwell equations
Membranes
Poisson equation
Cell membrane interactions
Electrochemotherapy
Electroporation
Ion transports
Membrane electroporation
Membrane permeabilization
Nernst-Planck equations
Transmembrane potentials
Cytology
Article
biotechnology
cell membrane
cell volume
CHO cell line
electrochemotherapy
electroporation
erythrocyte
ion transport
lipid vesicle
mathematical model
membrane potential
nonhuman
animal
biological model
cell membrane
Cricetulus
electroporation
erythrocyte deformability
metabolism
physiology
procedures
calcium
chloride
Animals
Calcium
Cell Membrane
Chlorides
CHO Cells
Cricetulus
Electroporation
Erythrocyte Deformability
Erythrocytes
Ion Transport
Membrane Potentials
Models, Biological
Goldberg, E.
Suárez, C.
Alfonso, M.
Marchese, J.
Soba, A.
Marshall, G.
Cell membrane electroporation modeling: A multiphysics approach
topic_facet Electrochemotherapy
Electroporation
Ion transport
Mathematical modeling
Membrane deformation
Bioelectric potentials
Cells
Chlorine compounds
Electric fields
Ions
Mathematical models
Maxwell equations
Membranes
Poisson equation
Cell membrane interactions
Electrochemotherapy
Electroporation
Ion transports
Membrane electroporation
Membrane permeabilization
Nernst-Planck equations
Transmembrane potentials
Cytology
Article
biotechnology
cell membrane
cell volume
CHO cell line
electrochemotherapy
electroporation
erythrocyte
ion transport
lipid vesicle
mathematical model
membrane potential
nonhuman
animal
biological model
cell membrane
Cricetulus
electroporation
erythrocyte deformability
metabolism
physiology
procedures
calcium
chloride
Animals
Calcium
Cell Membrane
Chlorides
CHO Cells
Cricetulus
Electroporation
Erythrocyte Deformability
Erythrocytes
Ion Transport
Membrane Potentials
Models, Biological
description Electroporation-based techniques, i.e. techniques based on the perturbation of the cell membrane through the application of electric pulses, are widely used at present in medicine and biotechnology. However, the electric pulse - cell membrane interaction is not yet completely understood neither explicitly formalized. Here we introduce a Multiphysics (MP) model describing electric pulse - cell membrane interaction consisting on the Poisson equation for the electric field, the Nernst-Planck equations for ion transport (protons, hydroxides, sodium or calcium, and chloride), the Maxwell tensor and mechanical equilibrium equation for membrane deformations (with an explicit discretization of the cell membrane), and the Smoluchowski equation for membrane permeabilization. The MP model predicts that during the application of an electric pulse to a spherical cell an elastic deformation of its membrane takes place affecting the induced transmembrane potential, the pore creation dynamics and the ionic transport. Moreover, the coincidence among maximum membrane deformation, maximum pore aperture, and maximum ion uptake is predicted. Such behavior has been corroborated experimentally by previously published results in red blood and CHO cells as well as in supramolecular lipid vesicles. © 2018
format JOUR
author Goldberg, E.
Suárez, C.
Alfonso, M.
Marchese, J.
Soba, A.
Marshall, G.
author_facet Goldberg, E.
Suárez, C.
Alfonso, M.
Marchese, J.
Soba, A.
Marshall, G.
author_sort Goldberg, E.
title Cell membrane electroporation modeling: A multiphysics approach
title_short Cell membrane electroporation modeling: A multiphysics approach
title_full Cell membrane electroporation modeling: A multiphysics approach
title_fullStr Cell membrane electroporation modeling: A multiphysics approach
title_full_unstemmed Cell membrane electroporation modeling: A multiphysics approach
title_sort cell membrane electroporation modeling: a multiphysics approach
url http://hdl.handle.net/20.500.12110/paper_15675394_v124_n_p28_Goldberg
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AT suarezc cellmembraneelectroporationmodelingamultiphysicsapproach
AT alfonsom cellmembraneelectroporationmodelingamultiphysicsapproach
AT marchesej cellmembraneelectroporationmodelingamultiphysicsapproach
AT sobaa cellmembraneelectroporationmodelingamultiphysicsapproach
AT marshallg cellmembraneelectroporationmodelingamultiphysicsapproach
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