Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse
During early placentation, matrix metalloproteinases (MMPs) play important roles in decidualization, trophoblast migration, invasion, angiogenesis, vascularization and extracellular matrix (ECM) remodeling of the endometrium. The aim of our study was to analyze the localization, distribution and dif...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_15672379_v43_n5_p487_Fontana |
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todo:paper_15672379_v43_n5_p487_Fontana2023-10-03T16:26:35Z Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse Fontana, V. Coll, T.A. Sobarzo, C.M.A. Tito, L.P. Calvo, J.C. Cebral, E. CF-1 mouse Decidua Matrix metalloproteinases Organogenesis gelatinase A gelatinase B matrix metalloproteinase animal tissue article controlled study culture medium cytoplasm decidua embryo endometrium enzyme activation enzyme activity extracellular matrix female in vitro study in vivo study mouse nonhuman organogenesis priority journal protein expression protein localization trophoblast Western blotting Animals Decidua Embryo Implantation Endometrium Extracellular Matrix Female Gene Expression Regulation, Developmental Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Organogenesis Placentation Pregnancy Trophoblasts During early placentation, matrix metalloproteinases (MMPs) play important roles in decidualization, trophoblast migration, invasion, angiogenesis, vascularization and extracellular matrix (ECM) remodeling of the endometrium. The aim of our study was to analyze the localization, distribution and differential expression of MMP-2 and -9 in the organogenic implantation site and to evaluate in vivo and in vitro decidual MMP-2 and -9 activities on day 10 of gestation in CF-1 mouse. Whole extracts for Western blotting of organogenic E10-decidua expressed MMP-2 and -9 isoforms. MMP-2 immunoreactivity was found in a granular and discrete pattern in ECM of mesometrial decidua (MD) near maternal blood vessels and slightly in non-decidualized endometrium (NDE). Immunoexpression of MMP-9 was also detected in NDE, in cytoplasm of decidual cells and ECM of vascular MD, in trophoblastic area and in growing antimesometrial deciduum. Gelatin zymography showed that MMP-9 activity was significantly lower in CM compared to the active form of direct (not cultured) and cultured decidua. The decidual active MMP-9 was significantly higher than the active MMP-2. These results show differential localization, protein expression and enzymatic activation of MMPs, suggesting specific roles for MMP-2 and MMP-9 in decidual and trophoblast tissues related to organogenic ECM remodeling and vascularization during early establishment of mouse placentation. © Springer Science+Business Media B.V. 2012. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15672379_v43_n5_p487_Fontana |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CF-1 mouse Decidua Matrix metalloproteinases Organogenesis gelatinase A gelatinase B matrix metalloproteinase animal tissue article controlled study culture medium cytoplasm decidua embryo endometrium enzyme activation enzyme activity extracellular matrix female in vitro study in vivo study mouse nonhuman organogenesis priority journal protein expression protein localization trophoblast Western blotting Animals Decidua Embryo Implantation Endometrium Extracellular Matrix Female Gene Expression Regulation, Developmental Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Organogenesis Placentation Pregnancy Trophoblasts |
| spellingShingle |
CF-1 mouse Decidua Matrix metalloproteinases Organogenesis gelatinase A gelatinase B matrix metalloproteinase animal tissue article controlled study culture medium cytoplasm decidua embryo endometrium enzyme activation enzyme activity extracellular matrix female in vitro study in vivo study mouse nonhuman organogenesis priority journal protein expression protein localization trophoblast Western blotting Animals Decidua Embryo Implantation Endometrium Extracellular Matrix Female Gene Expression Regulation, Developmental Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Organogenesis Placentation Pregnancy Trophoblasts Fontana, V. Coll, T.A. Sobarzo, C.M.A. Tito, L.P. Calvo, J.C. Cebral, E. Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse |
| topic_facet |
CF-1 mouse Decidua Matrix metalloproteinases Organogenesis gelatinase A gelatinase B matrix metalloproteinase animal tissue article controlled study culture medium cytoplasm decidua embryo endometrium enzyme activation enzyme activity extracellular matrix female in vitro study in vivo study mouse nonhuman organogenesis priority journal protein expression protein localization trophoblast Western blotting Animals Decidua Embryo Implantation Endometrium Extracellular Matrix Female Gene Expression Regulation, Developmental Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Organogenesis Placentation Pregnancy Trophoblasts |
| description |
During early placentation, matrix metalloproteinases (MMPs) play important roles in decidualization, trophoblast migration, invasion, angiogenesis, vascularization and extracellular matrix (ECM) remodeling of the endometrium. The aim of our study was to analyze the localization, distribution and differential expression of MMP-2 and -9 in the organogenic implantation site and to evaluate in vivo and in vitro decidual MMP-2 and -9 activities on day 10 of gestation in CF-1 mouse. Whole extracts for Western blotting of organogenic E10-decidua expressed MMP-2 and -9 isoforms. MMP-2 immunoreactivity was found in a granular and discrete pattern in ECM of mesometrial decidua (MD) near maternal blood vessels and slightly in non-decidualized endometrium (NDE). Immunoexpression of MMP-9 was also detected in NDE, in cytoplasm of decidual cells and ECM of vascular MD, in trophoblastic area and in growing antimesometrial deciduum. Gelatin zymography showed that MMP-9 activity was significantly lower in CM compared to the active form of direct (not cultured) and cultured decidua. The decidual active MMP-9 was significantly higher than the active MMP-2. These results show differential localization, protein expression and enzymatic activation of MMPs, suggesting specific roles for MMP-2 and MMP-9 in decidual and trophoblast tissues related to organogenic ECM remodeling and vascularization during early establishment of mouse placentation. © Springer Science+Business Media B.V. 2012. |
| format |
JOUR |
| author |
Fontana, V. Coll, T.A. Sobarzo, C.M.A. Tito, L.P. Calvo, J.C. Cebral, E. |
| author_facet |
Fontana, V. Coll, T.A. Sobarzo, C.M.A. Tito, L.P. Calvo, J.C. Cebral, E. |
| author_sort |
Fontana, V. |
| title |
Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse |
| title_short |
Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse |
| title_full |
Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse |
| title_fullStr |
Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse |
| title_full_unstemmed |
Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse |
| title_sort |
matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in cf-1 mouse |
| url |
http://hdl.handle.net/20.500.12110/paper_15672379_v43_n5_p487_Fontana |
| work_keys_str_mv |
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1807314736623124480 |