Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco

Mostrar todas las versiones(2)

The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populatio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Piccinali, R.V., Marcet, P.L., Ceballos, L.A., Kitron, U., Gürtler, R.E., Dotson, E.M.
Formato:	JOUR
Materias:	Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15671348_v11_n5_p895_Piccinali
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_15671348_v11_n5_p895_Piccinali
record_format	dspace
spelling	todo:paper_15671348_v11_n5_p895_Piccinali2023-10-03T16:26:29Z Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco Piccinali, R.V. Marcet, P.L. Ceballos, L.A. Kitron, U. Gürtler, R.E. Dotson, E.M. Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Triatoma infestans Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma Triatoma infestans The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populations in the recolonization process of insecticide-treated houses. The finding of a population of sylvatic melanic T. infestans (dark morphs) in the Argentinean dry Chaco at 7. km from a peridomestic bug population of typical coloration gave us the opportunity to test both questions simultaneously by employing phylogenetic and population genetic approaches. For this purpose we analyzed sylvatic and peridomestic bugs using sequence-based mitochondrial and nuclear markers (mtCOI and ITS-1) and microsatellites. Sylvatic bugs were confirmed to be T. infestans and not hybrids, and showed high levels of genetic variability and departures from neutral expectations for mtCOI variation. New ITS-1 and mtCOI haplotypes were recorded, as well as haplotypes shared with peridomestic and/or domestic bugs from previous records. The peridomestic population was invariant for ITS-1 and mtCOI, but showed variability for microsatellites and signatures of a population bottleneck, probably due to a limited number of founders. Phylogenetic analyses were consistent with the presence of ancestral haplotypes in sylvatic bugs. According to F-statistics and assignment methods there was a significant differentiation between sylvatic and peridomestic bugs and gene flow was low and asymmetric, with more bugs moving from the peridomicile to the sylvatic environment. These results support the hypothesis of the Chaco region as the area of origin of T. infestans, and a limited role of sylvatic melanic T. infestans in peridomestic infestation in the Argentinean Chaco. © 2011 Elsevier B.V. Fil:Piccinali, R.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marcet, P.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15671348_v11_n5_p895_Piccinali
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Triatoma infestans Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma Triatoma infestans
spellingShingle	Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Triatoma infestans Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma Triatoma infestans Piccinali, R.V. Marcet, P.L. Ceballos, L.A. Kitron, U. Gürtler, R.E. Dotson, E.M. Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco
topic_facet	Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Triatoma infestans Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma Triatoma infestans
description	The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populations in the recolonization process of insecticide-treated houses. The finding of a population of sylvatic melanic T. infestans (dark morphs) in the Argentinean dry Chaco at 7. km from a peridomestic bug population of typical coloration gave us the opportunity to test both questions simultaneously by employing phylogenetic and population genetic approaches. For this purpose we analyzed sylvatic and peridomestic bugs using sequence-based mitochondrial and nuclear markers (mtCOI and ITS-1) and microsatellites. Sylvatic bugs were confirmed to be T. infestans and not hybrids, and showed high levels of genetic variability and departures from neutral expectations for mtCOI variation. New ITS-1 and mtCOI haplotypes were recorded, as well as haplotypes shared with peridomestic and/or domestic bugs from previous records. The peridomestic population was invariant for ITS-1 and mtCOI, but showed variability for microsatellites and signatures of a population bottleneck, probably due to a limited number of founders. Phylogenetic analyses were consistent with the presence of ancestral haplotypes in sylvatic bugs. According to F-statistics and assignment methods there was a significant differentiation between sylvatic and peridomestic bugs and gene flow was low and asymmetric, with more bugs moving from the peridomicile to the sylvatic environment. These results support the hypothesis of the Chaco region as the area of origin of T. infestans, and a limited role of sylvatic melanic T. infestans in peridomestic infestation in the Argentinean Chaco. © 2011 Elsevier B.V.
format	JOUR
author	Piccinali, R.V. Marcet, P.L. Ceballos, L.A. Kitron, U. Gürtler, R.E. Dotson, E.M.
author_facet	Piccinali, R.V. Marcet, P.L. Ceballos, L.A. Kitron, U. Gürtler, R.E. Dotson, E.M.
author_sort	Piccinali, R.V.
title	Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco
title_short	Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco
title_full	Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco
title_fullStr	Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco
title_full_unstemmed	Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco
title_sort	genetic variability, phylogenetic relationships and gene flow in triatoma infestans dark morphs from the argentinean chaco
url	http://hdl.handle.net/20.500.12110/paper_15671348_v11_n5_p895_Piccinali
work_keys_str_mv	AT piccinalirv geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco AT marcetpl geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco AT ceballosla geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco AT kitronu geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco AT gurtlerre geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco AT dotsonem geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco
_version_	1807321560695963648

Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco

Ejemplares similares