Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish

Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveli...

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Autores principales:	Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., Reyes, A.E.
Formato:	JOUR
Materias:	Blood vessels Brain Cartilage Dorsal aorta Hypoxia-inducible factor Intersegmental vessels Neuromasts Notochord Optic tectum Ventricle epithelia Zebrafish hypoxia inducible factor 1alpha hypoxia inducible factor 2alpha messenger RNA oxygen basic helix loop helix transcription factor endothelial PAS domain containing protein 1 endothelial PAS domain-containing protein 1 zebrafish protein angiogenesis animal cell article blood vessel brain tissue controlled study developmental stage embryo embryo development expressed sequence tag gene expression gene expression regulation gene function genetic transcription heart heart development hypoxia molecular cloning molecular mechanics nervous system development nonhuman notochord nucleotide sequence oxygen tension phylogenetic tree priority journal protein domain reverse transcription polymerase chain reaction sequence analysis sequence homology species difference vertebrate zebra fish animal animal embryo antibody specificity gene expression profiling genetics in situ hybridization metabolism phylogeny prenatal development Danio rerio Vertebrata Animals Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit In Situ Hybridization Organ Specificity Phylogeny Zebrafish Proteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1567133X_v7_n3_p339_Rojas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_1567133X_v7_n3_p339_Rojas
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spelling	todo:paper_1567133X_v7_n3_p339_Rojas2023-10-03T16:26:28Z Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish Rojas, D.A. Perez-Munizaga, D.A. Centanin, L. Antonelli, M. Wappner, P. Allende, M.L. Reyes, A.E. Blood vessels Brain Cartilage Dorsal aorta Hypoxia-inducible factor Intersegmental vessels Neuromasts Notochord Optic tectum Ventricle epithelia Zebrafish hypoxia inducible factor 1alpha hypoxia inducible factor 2alpha messenger RNA oxygen basic helix loop helix transcription factor endothelial PAS domain containing protein 1 endothelial PAS domain-containing protein 1 hypoxia inducible factor 1alpha zebrafish protein angiogenesis animal cell article blood vessel brain tissue controlled study developmental stage embryo embryo development expressed sequence tag gene expression gene expression regulation gene function genetic transcription heart heart development hypoxia molecular cloning molecular mechanics nervous system development nonhuman notochord nucleotide sequence oxygen tension phylogenetic tree priority journal protein domain reverse transcription polymerase chain reaction sequence analysis sequence homology species difference vertebrate zebra fish animal animal embryo antibody specificity gene expression profiling gene expression regulation genetics in situ hybridization metabolism molecular cloning phylogeny prenatal development zebra fish Danio rerio Vertebrata Animals Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit In Situ Hybridization Organ Specificity Phylogeny Zebrafish Zebrafish Proteins Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1α and hif-2α genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1α and hif-2α genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1α and hif-2α are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1α is switched off, while hif-2α transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1α and hif-2α is directly assessed in a vertebrate model system throughout development. © 2006 Elsevier B.V. All rights reserved. Fil:Centanin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wappner, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1567133X_v7_n3_p339_Rojas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Blood vessels Brain Cartilage Dorsal aorta Hypoxia-inducible factor Intersegmental vessels Neuromasts Notochord Optic tectum Ventricle epithelia Zebrafish hypoxia inducible factor 1alpha hypoxia inducible factor 2alpha messenger RNA oxygen basic helix loop helix transcription factor endothelial PAS domain containing protein 1 endothelial PAS domain-containing protein 1 hypoxia inducible factor 1alpha zebrafish protein angiogenesis animal cell article blood vessel brain tissue controlled study developmental stage embryo embryo development expressed sequence tag gene expression gene expression regulation gene function genetic transcription heart heart development hypoxia molecular cloning molecular mechanics nervous system development nonhuman notochord nucleotide sequence oxygen tension phylogenetic tree priority journal protein domain reverse transcription polymerase chain reaction sequence analysis sequence homology species difference vertebrate zebra fish animal animal embryo antibody specificity gene expression profiling gene expression regulation genetics in situ hybridization metabolism molecular cloning phylogeny prenatal development zebra fish Danio rerio Vertebrata Animals Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit In Situ Hybridization Organ Specificity Phylogeny Zebrafish Zebrafish Proteins
spellingShingle	Blood vessels Brain Cartilage Dorsal aorta Hypoxia-inducible factor Intersegmental vessels Neuromasts Notochord Optic tectum Ventricle epithelia Zebrafish hypoxia inducible factor 1alpha hypoxia inducible factor 2alpha messenger RNA oxygen basic helix loop helix transcription factor endothelial PAS domain containing protein 1 endothelial PAS domain-containing protein 1 hypoxia inducible factor 1alpha zebrafish protein angiogenesis animal cell article blood vessel brain tissue controlled study developmental stage embryo embryo development expressed sequence tag gene expression gene expression regulation gene function genetic transcription heart heart development hypoxia molecular cloning molecular mechanics nervous system development nonhuman notochord nucleotide sequence oxygen tension phylogenetic tree priority journal protein domain reverse transcription polymerase chain reaction sequence analysis sequence homology species difference vertebrate zebra fish animal animal embryo antibody specificity gene expression profiling gene expression regulation genetics in situ hybridization metabolism molecular cloning phylogeny prenatal development zebra fish Danio rerio Vertebrata Animals Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit In Situ Hybridization Organ Specificity Phylogeny Zebrafish Zebrafish Proteins Rojas, D.A. Perez-Munizaga, D.A. Centanin, L. Antonelli, M. Wappner, P. Allende, M.L. Reyes, A.E. Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
topic_facet	Blood vessels Brain Cartilage Dorsal aorta Hypoxia-inducible factor Intersegmental vessels Neuromasts Notochord Optic tectum Ventricle epithelia Zebrafish hypoxia inducible factor 1alpha hypoxia inducible factor 2alpha messenger RNA oxygen basic helix loop helix transcription factor endothelial PAS domain containing protein 1 endothelial PAS domain-containing protein 1 hypoxia inducible factor 1alpha zebrafish protein angiogenesis animal cell article blood vessel brain tissue controlled study developmental stage embryo embryo development expressed sequence tag gene expression gene expression regulation gene function genetic transcription heart heart development hypoxia molecular cloning molecular mechanics nervous system development nonhuman notochord nucleotide sequence oxygen tension phylogenetic tree priority journal protein domain reverse transcription polymerase chain reaction sequence analysis sequence homology species difference vertebrate zebra fish animal animal embryo antibody specificity gene expression profiling gene expression regulation genetics in situ hybridization metabolism molecular cloning phylogeny prenatal development zebra fish Danio rerio Vertebrata Animals Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit In Situ Hybridization Organ Specificity Phylogeny Zebrafish Zebrafish Proteins
description	Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1α and hif-2α genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1α and hif-2α genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1α and hif-2α are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1α is switched off, while hif-2α transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1α and hif-2α is directly assessed in a vertebrate model system throughout development. © 2006 Elsevier B.V. All rights reserved.
format	JOUR
author	Rojas, D.A. Perez-Munizaga, D.A. Centanin, L. Antonelli, M. Wappner, P. Allende, M.L. Reyes, A.E.
author_facet	Rojas, D.A. Perez-Munizaga, D.A. Centanin, L. Antonelli, M. Wappner, P. Allende, M.L. Reyes, A.E.
author_sort	Rojas, D.A.
title	Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
title_short	Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
title_full	Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
title_fullStr	Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
title_full_unstemmed	Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
title_sort	cloning of hif-1α and hif-2α and mrna expression pattern during development in zebrafish
url	http://hdl.handle.net/20.500.12110/paper_1567133X_v7_n3_p339_Rojas
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Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish

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