The arabidopsis DNA polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering
DNA replication is a key process in living organisms. DNA polymerase α (Polα) initiates strand synthesis, which is performed by Polε and Polδ in leading and lagging strands, respectively. Whereas loss of DNA polymerase activity is incompatible with life, viable mutants of Polα and Polε were isolated...
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todo:paper_15537404_v11_n2_pe1004975_Iglesias2023-10-03T16:25:33Z The arabidopsis DNA polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering Iglesias, F.M. Bruera, N.A. Dergan-Dylon, S. Marino-Buslje, C. Lorenzi, H. Mateos, J.L. Turck, F. Coupland, G. Cerdán, P.D. Arabidopsis protein DNA directed DNA polymerase gamma FT protein, Arabidopsis histone homeodomain protein MADS domain protein SEP3 protein, Arabidopsis transcription factor Arabidopsis biosynthesis DNA replication flower gene expression regulation genetic epigenesis genetics growth, development and aging plant leaf Arabidopsis Arabidopsis Proteins DNA Polymerase III DNA Replication Epigenesis, Genetic Flowers Gene Expression Regulation, Plant Histones Homeodomain Proteins MADS Domain Proteins Plant Leaves Transcription Factors DNA replication is a key process in living organisms. DNA polymerase α (Polα) initiates strand synthesis, which is performed by Polε and Polδ in leading and lagging strands, respectively. Whereas loss of DNA polymerase activity is incompatible with life, viable mutants of Polα and Polε were isolated, allowing the identification of their functions beyond DNA replication. In contrast, no viable mutants in the Polδ polymerase-domain were reported in multicellular organisms. Here we identify such a mutant which is also thermosensitive. Mutant plants were unable to complete development at 28°C, looked normal at 18°C, but displayed increased expression of DNA replication-stress marker genes, homologous recombination and lysine 4 histone 3 trimethylation at the SEPALLATA3 (SEP3) locus at 24°C, which correlated with ectopic expression of SEP3. Surprisingly, high expression of SEP3 in vascular tissue promoted FLOWERING LOCUS T (FT) expression, forming a positive feedback loop with SEP3 and leading to early flowering and curly leaves phenotypes. These results strongly suggest that the DNA polymerase δ is required for the proper establishment of transcriptionally active epigenetic marks and that its failure might affect development by affecting the epigenetic control of master genes. Fil:Iglesias, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lorenzi, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerdán, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15537404_v11_n2_pe1004975_Iglesias |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arabidopsis protein DNA directed DNA polymerase gamma FT protein, Arabidopsis histone homeodomain protein MADS domain protein SEP3 protein, Arabidopsis transcription factor Arabidopsis biosynthesis DNA replication flower gene expression regulation genetic epigenesis genetics growth, development and aging plant leaf Arabidopsis Arabidopsis Proteins DNA Polymerase III DNA Replication Epigenesis, Genetic Flowers Gene Expression Regulation, Plant Histones Homeodomain Proteins MADS Domain Proteins Plant Leaves Transcription Factors |
| spellingShingle |
Arabidopsis protein DNA directed DNA polymerase gamma FT protein, Arabidopsis histone homeodomain protein MADS domain protein SEP3 protein, Arabidopsis transcription factor Arabidopsis biosynthesis DNA replication flower gene expression regulation genetic epigenesis genetics growth, development and aging plant leaf Arabidopsis Arabidopsis Proteins DNA Polymerase III DNA Replication Epigenesis, Genetic Flowers Gene Expression Regulation, Plant Histones Homeodomain Proteins MADS Domain Proteins Plant Leaves Transcription Factors Iglesias, F.M. Bruera, N.A. Dergan-Dylon, S. Marino-Buslje, C. Lorenzi, H. Mateos, J.L. Turck, F. Coupland, G. Cerdán, P.D. The arabidopsis DNA polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering |
| topic_facet |
Arabidopsis protein DNA directed DNA polymerase gamma FT protein, Arabidopsis histone homeodomain protein MADS domain protein SEP3 protein, Arabidopsis transcription factor Arabidopsis biosynthesis DNA replication flower gene expression regulation genetic epigenesis genetics growth, development and aging plant leaf Arabidopsis Arabidopsis Proteins DNA Polymerase III DNA Replication Epigenesis, Genetic Flowers Gene Expression Regulation, Plant Histones Homeodomain Proteins MADS Domain Proteins Plant Leaves Transcription Factors |
| description |
DNA replication is a key process in living organisms. DNA polymerase α (Polα) initiates strand synthesis, which is performed by Polε and Polδ in leading and lagging strands, respectively. Whereas loss of DNA polymerase activity is incompatible with life, viable mutants of Polα and Polε were isolated, allowing the identification of their functions beyond DNA replication. In contrast, no viable mutants in the Polδ polymerase-domain were reported in multicellular organisms. Here we identify such a mutant which is also thermosensitive. Mutant plants were unable to complete development at 28°C, looked normal at 18°C, but displayed increased expression of DNA replication-stress marker genes, homologous recombination and lysine 4 histone 3 trimethylation at the SEPALLATA3 (SEP3) locus at 24°C, which correlated with ectopic expression of SEP3. Surprisingly, high expression of SEP3 in vascular tissue promoted FLOWERING LOCUS T (FT) expression, forming a positive feedback loop with SEP3 and leading to early flowering and curly leaves phenotypes. These results strongly suggest that the DNA polymerase δ is required for the proper establishment of transcriptionally active epigenetic marks and that its failure might affect development by affecting the epigenetic control of master genes. |
| format |
JOUR |
| author |
Iglesias, F.M. Bruera, N.A. Dergan-Dylon, S. Marino-Buslje, C. Lorenzi, H. Mateos, J.L. Turck, F. Coupland, G. Cerdán, P.D. |
| author_facet |
Iglesias, F.M. Bruera, N.A. Dergan-Dylon, S. Marino-Buslje, C. Lorenzi, H. Mateos, J.L. Turck, F. Coupland, G. Cerdán, P.D. |
| author_sort |
Iglesias, F.M. |
| title |
The arabidopsis DNA polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering |
| title_short |
The arabidopsis DNA polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering |
| title_full |
The arabidopsis DNA polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering |
| title_fullStr |
The arabidopsis DNA polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering |
| title_full_unstemmed |
The arabidopsis DNA polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering |
| title_sort |
arabidopsis dna polymerase δ has a role in the deposition of transcriptionally active epigenetic marks, development and flowering |
| url |
http://hdl.handle.net/20.500.12110/paper_15537404_v11_n2_pe1004975_Iglesias |
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