Identification and Functional Analysis of Healing Regulators in Drosophila

Wound healing is an essential homeostatic mechanism that maintains the epithelial barrier integrity after tissue damage. Although we know the overall steps in wound healing, many of the underlying molecular mechanisms remain unclear. Genetically amenable systems, such as wound healing in Drosophila...

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Autores principales:	Álvarez-Fernández, C., Tamirisa, S., Prada, F., Chernomoretz, A., Podhajcer, O., Blanco, E., Martín-Blanco, E.
Formato:	JOUR
Materias:	chaperonin containing TCP1 actin stress activated protein kinase actin filament adult animal cell animal tissue arc1 gene Article chemical analysis controlled study Drosophila female gene gene expression profiling gene function gene identification gene overexpression gene product genetic analysis in vitro study male nonhuman nucleotide sequence PDGF gene rho1 gene serpin55B gene tissue repair VEGF gene wound healing animal cytoskeleton Drosophila melanogaster epithelium gene expression regulation genetics growth, development and aging human imaginal disc insect genome metabolism pathology regeneration signal transduction thorax Mammalia Murinae Actins Animals Cytoskeleton Epithelium Gene Expression Regulation Genome, Insect Humans Imaginal Discs JNK Mitogen-Activated Protein Kinases MAP Kinase Signaling System Regeneration Signal Transduction Thorax Wound Healing
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15537390_v11_n2_p1_AlvarezFernandez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_15537390_v11_n2_p1_AlvarezFernandez
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spelling	todo:paper_15537390_v11_n2_p1_AlvarezFernandez2023-10-03T16:25:31Z Identification and Functional Analysis of Healing Regulators in Drosophila Álvarez-Fernández, C. Tamirisa, S. Prada, F. Chernomoretz, A. Podhajcer, O. Blanco, E. Martín-Blanco, E. chaperonin containing TCP1 actin stress activated protein kinase actin filament adult animal cell animal tissue arc1 gene Article chemical analysis controlled study Drosophila female gene gene expression profiling gene function gene identification gene overexpression gene product genetic analysis in vitro study male nonhuman nucleotide sequence PDGF gene rho1 gene serpin55B gene tissue repair VEGF gene wound healing animal cytoskeleton Drosophila melanogaster epithelium gene expression regulation genetics growth, development and aging human imaginal disc insect genome metabolism pathology regeneration signal transduction thorax wound healing Mammalia Murinae Actins Animals Cytoskeleton Drosophila melanogaster Epithelium Gene Expression Regulation Genome, Insect Humans Imaginal Discs JNK Mitogen-Activated Protein Kinases MAP Kinase Signaling System Regeneration Signal Transduction Thorax Wound Healing Wound healing is an essential homeostatic mechanism that maintains the epithelial barrier integrity after tissue damage. Although we know the overall steps in wound healing, many of the underlying molecular mechanisms remain unclear. Genetically amenable systems, such as wound healing in Drosophila imaginal discs, do not model all aspects of the repair process. However, they do allow the less understood aspects of the healing response to be explored, e.g., which signal(s) are responsible for initiating tissue remodeling? How is sealing of the epithelia achieved? Or, what inhibitory cues cancel the healing machinery upon completion? Answering these and other questions first requires the identification and functional analysis of wound specific genes. A variety of different microarray analyses of murine and humans have identified characteristic profiles of gene expression at the wound site, however, very few functional studies in healing regulation have been carried out. We developed an experimentally controlled method that is healing-permissive and that allows live imaging and biochemical analysis of cultured imaginal discs. We performed comparative genome-wide profiling between Drosophila imaginal cells actively involved in healing versus their non-engaged siblings. Sets of potential wound-specific genes were subsequently identified. Importantly, besides identifying and categorizing new genes, we functionally tested many of their gene products by genetic interference and overexpression in healing assays. This non-saturated analysis defines a relevant set of genes whose changes in expression level are functionally significant for proper tissue repair. Amongst these we identified the TCP1 chaperonin complex as a key regulator of the actin cytoskeleton essential for the wound healing response. There is promise that our newly identified wound-healing genes will guide future work in the more complex mammalian wound healing response. © 2015 Álvarez-Fernández et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15537390_v11_n2_p1_AlvarezFernandez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	chaperonin containing TCP1 actin stress activated protein kinase actin filament adult animal cell animal tissue arc1 gene Article chemical analysis controlled study Drosophila female gene gene expression profiling gene function gene identification gene overexpression gene product genetic analysis in vitro study male nonhuman nucleotide sequence PDGF gene rho1 gene serpin55B gene tissue repair VEGF gene wound healing animal cytoskeleton Drosophila melanogaster epithelium gene expression regulation genetics growth, development and aging human imaginal disc insect genome metabolism pathology regeneration signal transduction thorax wound healing Mammalia Murinae Actins Animals Cytoskeleton Drosophila melanogaster Epithelium Gene Expression Regulation Genome, Insect Humans Imaginal Discs JNK Mitogen-Activated Protein Kinases MAP Kinase Signaling System Regeneration Signal Transduction Thorax Wound Healing
spellingShingle	chaperonin containing TCP1 actin stress activated protein kinase actin filament adult animal cell animal tissue arc1 gene Article chemical analysis controlled study Drosophila female gene gene expression profiling gene function gene identification gene overexpression gene product genetic analysis in vitro study male nonhuman nucleotide sequence PDGF gene rho1 gene serpin55B gene tissue repair VEGF gene wound healing animal cytoskeleton Drosophila melanogaster epithelium gene expression regulation genetics growth, development and aging human imaginal disc insect genome metabolism pathology regeneration signal transduction thorax wound healing Mammalia Murinae Actins Animals Cytoskeleton Drosophila melanogaster Epithelium Gene Expression Regulation Genome, Insect Humans Imaginal Discs JNK Mitogen-Activated Protein Kinases MAP Kinase Signaling System Regeneration Signal Transduction Thorax Wound Healing Álvarez-Fernández, C. Tamirisa, S. Prada, F. Chernomoretz, A. Podhajcer, O. Blanco, E. Martín-Blanco, E. Identification and Functional Analysis of Healing Regulators in Drosophila
topic_facet	chaperonin containing TCP1 actin stress activated protein kinase actin filament adult animal cell animal tissue arc1 gene Article chemical analysis controlled study Drosophila female gene gene expression profiling gene function gene identification gene overexpression gene product genetic analysis in vitro study male nonhuman nucleotide sequence PDGF gene rho1 gene serpin55B gene tissue repair VEGF gene wound healing animal cytoskeleton Drosophila melanogaster epithelium gene expression regulation genetics growth, development and aging human imaginal disc insect genome metabolism pathology regeneration signal transduction thorax wound healing Mammalia Murinae Actins Animals Cytoskeleton Drosophila melanogaster Epithelium Gene Expression Regulation Genome, Insect Humans Imaginal Discs JNK Mitogen-Activated Protein Kinases MAP Kinase Signaling System Regeneration Signal Transduction Thorax Wound Healing
description	Wound healing is an essential homeostatic mechanism that maintains the epithelial barrier integrity after tissue damage. Although we know the overall steps in wound healing, many of the underlying molecular mechanisms remain unclear. Genetically amenable systems, such as wound healing in Drosophila imaginal discs, do not model all aspects of the repair process. However, they do allow the less understood aspects of the healing response to be explored, e.g., which signal(s) are responsible for initiating tissue remodeling? How is sealing of the epithelia achieved? Or, what inhibitory cues cancel the healing machinery upon completion? Answering these and other questions first requires the identification and functional analysis of wound specific genes. A variety of different microarray analyses of murine and humans have identified characteristic profiles of gene expression at the wound site, however, very few functional studies in healing regulation have been carried out. We developed an experimentally controlled method that is healing-permissive and that allows live imaging and biochemical analysis of cultured imaginal discs. We performed comparative genome-wide profiling between Drosophila imaginal cells actively involved in healing versus their non-engaged siblings. Sets of potential wound-specific genes were subsequently identified. Importantly, besides identifying and categorizing new genes, we functionally tested many of their gene products by genetic interference and overexpression in healing assays. This non-saturated analysis defines a relevant set of genes whose changes in expression level are functionally significant for proper tissue repair. Amongst these we identified the TCP1 chaperonin complex as a key regulator of the actin cytoskeleton essential for the wound healing response. There is promise that our newly identified wound-healing genes will guide future work in the more complex mammalian wound healing response. © 2015 Álvarez-Fernández et al.
format	JOUR
author	Álvarez-Fernández, C. Tamirisa, S. Prada, F. Chernomoretz, A. Podhajcer, O. Blanco, E. Martín-Blanco, E.
author_facet	Álvarez-Fernández, C. Tamirisa, S. Prada, F. Chernomoretz, A. Podhajcer, O. Blanco, E. Martín-Blanco, E.
author_sort	Álvarez-Fernández, C.
title	Identification and Functional Analysis of Healing Regulators in Drosophila
title_short	Identification and Functional Analysis of Healing Regulators in Drosophila
title_full	Identification and Functional Analysis of Healing Regulators in Drosophila
title_fullStr	Identification and Functional Analysis of Healing Regulators in Drosophila
title_full_unstemmed	Identification and Functional Analysis of Healing Regulators in Drosophila
title_sort	identification and functional analysis of healing regulators in drosophila
url	http://hdl.handle.net/20.500.12110/paper_15537390_v11_n2_p1_AlvarezFernandez
work_keys_str_mv	AT alvarezfernandezc identificationandfunctionalanalysisofhealingregulatorsindrosophila AT tamirisas identificationandfunctionalanalysisofhealingregulatorsindrosophila AT pradaf identificationandfunctionalanalysisofhealingregulatorsindrosophila AT chernomoretza identificationandfunctionalanalysisofhealingregulatorsindrosophila AT podhajcero identificationandfunctionalanalysisofhealingregulatorsindrosophila AT blancoe identificationandfunctionalanalysisofhealingregulatorsindrosophila AT martinblancoe identificationandfunctionalanalysisofhealingregulatorsindrosophila
_version_	1807323194584989696

Identification and Functional Analysis of Healing Regulators in Drosophila

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