RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20
Previous studies have linked the C-terminal domain (CTD) of RNA polymerase II (pol II) with cotranscriptional precursor messenger RNA processing, but little is known about the CTD's function in regulating alternative splicing. We have examined this function using α-amanitin-resistant pol II CTD...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_15459993_v13_n11_p973_DeLaMata |
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todo:paper_15459993_v13_n11_p973_DeLaMata2023-10-03T16:23:05Z RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20 De La Mata, M. Kornblihtt, A.R. alpha amanitin arginine fibronectin messenger RNA protein p20 RNA polymerase II serine alternative RNA splicing article controlled study exon human human cell molecular dynamics phosphorylation priority journal protein domain protein function regulatory mechanism terminal sequence transcription regulation Alternative Splicing Amanitins Cell Line, Tumor Exons Fibronectins Gene Deletion Humans Phosphorylation Protein Structure, Tertiary RNA Polymerase II RNA-Binding Proteins Transcription, Genetic Transfection Previous studies have linked the C-terminal domain (CTD) of RNA polymerase II (pol II) with cotranscriptional precursor messenger RNA processing, but little is known about the CTD's function in regulating alternative splicing. We have examined this function using α-amanitin-resistant pol II CTD mutants and fibronectin reporter minigenes. We found that the CTD is required for the inhibitory action of the serine/arginine-rich (SR) protein SRp20 on the inclusion of a fibronectin cassette exon in the mature mRNA. CTD phosphorylation controls transcription elongation, which is a major contributor to alternative splicing regulation. However, the effect of SRp20 is still observed when transcription elongation is reduced. These results suggest that the CTD promotes exon skipping by recruiting SRp20 and that this contributes independently of elongation to the transcriptional control of alternative splicing. © 2006 Nature Publishing Group. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15459993_v13_n11_p973_DeLaMata |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alpha amanitin arginine fibronectin messenger RNA protein p20 RNA polymerase II serine alternative RNA splicing article controlled study exon human human cell molecular dynamics phosphorylation priority journal protein domain protein function regulatory mechanism terminal sequence transcription regulation Alternative Splicing Amanitins Cell Line, Tumor Exons Fibronectins Gene Deletion Humans Phosphorylation Protein Structure, Tertiary RNA Polymerase II RNA-Binding Proteins Transcription, Genetic Transfection |
| spellingShingle |
alpha amanitin arginine fibronectin messenger RNA protein p20 RNA polymerase II serine alternative RNA splicing article controlled study exon human human cell molecular dynamics phosphorylation priority journal protein domain protein function regulatory mechanism terminal sequence transcription regulation Alternative Splicing Amanitins Cell Line, Tumor Exons Fibronectins Gene Deletion Humans Phosphorylation Protein Structure, Tertiary RNA Polymerase II RNA-Binding Proteins Transcription, Genetic Transfection De La Mata, M. Kornblihtt, A.R. RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20 |
| topic_facet |
alpha amanitin arginine fibronectin messenger RNA protein p20 RNA polymerase II serine alternative RNA splicing article controlled study exon human human cell molecular dynamics phosphorylation priority journal protein domain protein function regulatory mechanism terminal sequence transcription regulation Alternative Splicing Amanitins Cell Line, Tumor Exons Fibronectins Gene Deletion Humans Phosphorylation Protein Structure, Tertiary RNA Polymerase II RNA-Binding Proteins Transcription, Genetic Transfection |
| description |
Previous studies have linked the C-terminal domain (CTD) of RNA polymerase II (pol II) with cotranscriptional precursor messenger RNA processing, but little is known about the CTD's function in regulating alternative splicing. We have examined this function using α-amanitin-resistant pol II CTD mutants and fibronectin reporter minigenes. We found that the CTD is required for the inhibitory action of the serine/arginine-rich (SR) protein SRp20 on the inclusion of a fibronectin cassette exon in the mature mRNA. CTD phosphorylation controls transcription elongation, which is a major contributor to alternative splicing regulation. However, the effect of SRp20 is still observed when transcription elongation is reduced. These results suggest that the CTD promotes exon skipping by recruiting SRp20 and that this contributes independently of elongation to the transcriptional control of alternative splicing. © 2006 Nature Publishing Group. |
| format |
JOUR |
| author |
De La Mata, M. Kornblihtt, A.R. |
| author_facet |
De La Mata, M. Kornblihtt, A.R. |
| author_sort |
De La Mata, M. |
| title |
RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20 |
| title_short |
RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20 |
| title_full |
RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20 |
| title_fullStr |
RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20 |
| title_full_unstemmed |
RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20 |
| title_sort |
rna polymerase ii c-terminal domain mediates regulation of alternative splicing by srp20 |
| url |
http://hdl.handle.net/20.500.12110/paper_15459993_v13_n11_p973_DeLaMata |
| work_keys_str_mv |
AT delamatam rnapolymeraseiicterminaldomainmediatesregulationofalternativesplicingbysrp20 AT kornblihttar rnapolymeraseiicterminaldomainmediatesregulationofalternativesplicingbysrp20 |
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