Comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation

The haloarchaea Natrialba magadii and Haloferax volcanii, as well as the radiation-resistant bacterium Deinococcus radiodurans, were exposed to vacuum UV (VUV) radiation at the Brazilian Synchrotron Light Laboratory. Cell monolayers (containing 10 5 to 10 6 cells per sample) were prepared over polyc...

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Autores principales: Abrevaya, X.C., Paulino-Lima, I.G., Galante, D., Rodrigues, F., Mauas, P.J.D., Cortón, E., Lage, C.D.A.S.
Formato: JOUR
Materias:
Panspermia
Planetary protection
Radiation resistance
Synchrotron
Vacuum ultraviolet
article
comparative study
Deinococcus
Halobacteriales
Haloferax volcanii
radiation exposure
survival
ultraviolet radiation
vacuum
Halobacteriaceae
Survival Analysis
Ultraviolet Rays
Vacuum
Bacteria (microorganisms)
Deinococcus radiodurans
Halobacteria
Natrialba magadii
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15311074_v11_n10_p1034_Abrevaya
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_15311074_v11_n10_p1034_Abrevaya2023-10-03T16:21:22Z Comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation Abrevaya, X.C. Paulino-Lima, I.G. Galante, D. Rodrigues, F. Mauas, P.J.D. Cortón, E. Lage, C.D.A.S. Panspermia Planetary protection Radiation resistance Synchrotron Vacuum ultraviolet article comparative study Deinococcus Halobacteriales Haloferax volcanii radiation exposure survival ultraviolet radiation vacuum Deinococcus Halobacteriaceae Haloferax volcanii Survival Analysis Ultraviolet Rays Vacuum Bacteria (microorganisms) Deinococcus radiodurans Halobacteria Haloferax volcanii Natrialba magadii The haloarchaea Natrialba magadii and Haloferax volcanii, as well as the radiation-resistant bacterium Deinococcus radiodurans, were exposed to vacuum UV (VUV) radiation at the Brazilian Synchrotron Light Laboratory. Cell monolayers (containing 10 5 to 10 6 cells per sample) were prepared over polycarbonate filters and irradiated under high vacuum (10 -5 Pa) with polychromatic synchrotron radiation. N. magadii was remarkably resistant to high vacuum with a survival fraction of (3.77±0.76)×10 -2, which was larger than that of D. radiodurans (1.13±0.23) ×10 -2. The survival fraction of the haloarchaea H. volcanii, of (3.60±1.80)×10 -4, was much smaller. Radiation resistance profiles were similar between the haloarchaea and D. radiodurans for fluences up to 150J m -2. For fluences larger than 150J m -2, there was a significant decrease in the survival of haloarchaea, and in particular H. volcanii did not survive. Survival for D. radiodurans was 1% after exposure to the higher VUV fluence (1350J m -2), while N. magadii had a survival lower than 0.1%. Such survival fractions are discussed regarding the possibility of interplanetary transfer of viable microorganisms and the possible existence of microbial life in extraterrestrial salty environments such as the planet Mars and Jupiter's moon Europa. This is the first work to report survival of haloarchaea under simulated interplanetary conditions. © Copyright 2011, Mary Ann Liebert, Inc. 2011. Fil:Abrevaya, X.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mauas, P.J.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15311074_v11_n10_p1034_Abrevaya
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Panspermia
Planetary protection
Radiation resistance
Synchrotron
Vacuum ultraviolet
article
comparative study
Deinococcus
Halobacteriales
Haloferax volcanii
radiation exposure
survival
ultraviolet radiation
vacuum
Deinococcus
Halobacteriaceae
Haloferax volcanii
Survival Analysis
Ultraviolet Rays
Vacuum
Bacteria (microorganisms)
Deinococcus radiodurans
Halobacteria
Haloferax volcanii
Natrialba magadii
spellingShingle Panspermia
Planetary protection
Radiation resistance
Synchrotron
Vacuum ultraviolet
article
comparative study
Deinococcus
Halobacteriales
Haloferax volcanii
radiation exposure
survival
ultraviolet radiation
vacuum
Deinococcus
Halobacteriaceae
Haloferax volcanii
Survival Analysis
Ultraviolet Rays
Vacuum
Bacteria (microorganisms)
Deinococcus radiodurans
Halobacteria
Haloferax volcanii
Natrialba magadii
Abrevaya, X.C.
Paulino-Lima, I.G.
Galante, D.
Rodrigues, F.
Mauas, P.J.D.
Cortón, E.
Lage, C.D.A.S.
Comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation
topic_facet Panspermia
Planetary protection
Radiation resistance
Synchrotron
Vacuum ultraviolet
article
comparative study
Deinococcus
Halobacteriales
Haloferax volcanii
radiation exposure
survival
ultraviolet radiation
vacuum
Deinococcus
Halobacteriaceae
Haloferax volcanii
Survival Analysis
Ultraviolet Rays
Vacuum
Bacteria (microorganisms)
Deinococcus radiodurans
Halobacteria
Haloferax volcanii
Natrialba magadii
description The haloarchaea Natrialba magadii and Haloferax volcanii, as well as the radiation-resistant bacterium Deinococcus radiodurans, were exposed to vacuum UV (VUV) radiation at the Brazilian Synchrotron Light Laboratory. Cell monolayers (containing 10 5 to 10 6 cells per sample) were prepared over polycarbonate filters and irradiated under high vacuum (10 -5 Pa) with polychromatic synchrotron radiation. N. magadii was remarkably resistant to high vacuum with a survival fraction of (3.77±0.76)×10 -2, which was larger than that of D. radiodurans (1.13±0.23) ×10 -2. The survival fraction of the haloarchaea H. volcanii, of (3.60±1.80)×10 -4, was much smaller. Radiation resistance profiles were similar between the haloarchaea and D. radiodurans for fluences up to 150J m -2. For fluences larger than 150J m -2, there was a significant decrease in the survival of haloarchaea, and in particular H. volcanii did not survive. Survival for D. radiodurans was 1% after exposure to the higher VUV fluence (1350J m -2), while N. magadii had a survival lower than 0.1%. Such survival fractions are discussed regarding the possibility of interplanetary transfer of viable microorganisms and the possible existence of microbial life in extraterrestrial salty environments such as the planet Mars and Jupiter's moon Europa. This is the first work to report survival of haloarchaea under simulated interplanetary conditions. © Copyright 2011, Mary Ann Liebert, Inc. 2011.
format JOUR
author Abrevaya, X.C.
Paulino-Lima, I.G.
Galante, D.
Rodrigues, F.
Mauas, P.J.D.
Cortón, E.
Lage, C.D.A.S.
author_facet Abrevaya, X.C.
Paulino-Lima, I.G.
Galante, D.
Rodrigues, F.
Mauas, P.J.D.
Cortón, E.
Lage, C.D.A.S.
author_sort Abrevaya, X.C.
title Comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation
title_short Comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation
title_full Comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation
title_fullStr Comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation
title_full_unstemmed Comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation
title_sort comparative survival analysis of deinococcus radiodurans and the haloarchaea natrialba magadii and haloferax volcanii exposed to vacuum ultraviolet irradiation
url http://hdl.handle.net/20.500.12110/paper_15311074_v11_n10_p1034_Abrevaya
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